Cloning and molecular characterization of Myb transcription factors from Leymus (Poaceae: Trticeae)
-
Ying-xia Lei
Abstract
Plant Myb transcription factors are crucial in regulating secondary metabolism, cellular morphogenesis and responses to hormone and stress signals. In this study, 19 Myb gene fragments were isolated from five Leymus species, three of which, LrMyb3, LmMyb8 and LmMyb6 were nearly complete open reading frames encoding putative Leymus Myb genes. Sequence and phylogenetic analysis indicated that all these putative Myb from Leymus represent plant typical R2R3-Myb genes. Compared with Myb gene in Arabidopsis thaliana, the 19 Leymus Myb gene fragments of the R2R3 region amino acid showed three different types, W-x (19) W-x (19) W-x (12) F-x (18) W-x (18) W, W-x (19) W-x (19) W-x (12) M-x (18) W-x (18) W and W-x (19) W-x (19) R-x (12) L-x (18) W-x (18) W. LmMyb8 gene shares high similarity to ZmMyb39 which was possible involvement of caffeic acid O-methyl-transferase (COMT) in the lignification process. TheLmMyb8 gene is expressed at high level in leaves and roots while at low level in stems.
Acknowledgements
The authors are very grateful to the American National Plant Germplasm System for kindly providing some of seeds in this study. We are thankful to the National Natural Science Foundation of China (Grant Nos. 31470305, 31270243) and the Science and Technology Bureau and Education Bureau of Sichuan Province, China for their financial support.
References
Aasland R., Stewart A.F. & Gibson T. 1996. The SANT domain: a putative DNA-binding domain in the SWI-SNF and ADA complexes, the transcriptional co-repressor N-CoR and TFIIIB. Trends Biochem. Sci. 21: 87–88.10.1016/0968-0004(96)30009-1Suche in Google Scholar
Agarwal M., Hao Y., Kapoor A., Dong C.H., Fuji H, Zheng X. & Zhu J.K. 2006. A R2R3 type MYB transcription factor is involved in the cold regulation of CBF genes and in acquired freezing tolerance. J. Biol. Chem. 281: 37636–37645.10.1074/jbc.M605895200Suche in Google Scholar PubMed
Azuma A., Kobayashi S., Mitani N., Shiraishi M., Yamada M., Ueno T., Kono A., Yakushiji H. & Koshita Y. 2008. Genomic and genetic analysis of Myb-related genes that regulate anthocyanin biosynthesis in grape berry skin. Theor. Appl. Genet 117: 1009–1019.10.1007/s00122-008-0840-1Suche in Google Scholar PubMed
Bedon F., Grima-Pettenati J. & Mackay J. 2007. Conifer R2R3-Myb transcription factors: sequence analyses and gene expression in wood-forming tissues of white spruce (Picea glauca). BMC Plant Biol. 7: 17.10.1186/1471-2229-7-17Suche in Google Scholar PubMed PubMed Central
Bailey P.C., Dicks J., Wang T. & Martin C. 2008. IT3F: A web-based wool for functional analysis of transcription factors in plants. Phytochemistry 69: 2417–2425.10.1016/j.phytochem.2008.08.003Suche in Google Scholar PubMed
Bushman B.S., Larson S.R., Mott L.W., Cliften P.F., Wang R.R.C., Chatterton N.J., Hernandez A.G., Ali S., Kim R.W., Thimmapuram J., Gong G., Liu L. & Mikel M.A. 2008. Development and annotation of perennial Triticeae ESTSs and SSR markers. Genome 51: 779–788.10.1139/G08-062Suche in Google Scholar PubMed
Cedroni M.L., Cronn R.C., Adams K.L., Wilkins T.A. & Wendel J.F. 2003. Evolution and expression of Myb genes in diploid and polyploidy cotton. Plant Mol. Biol.51: 313–325.10.1023/A:1022051100610Suche in Google Scholar
Chen J. & Wang Z.Y. 2002. Progress in the study of plant transcription factors. J Plant Physiol. Mol. Biol 28: 81–88.Suche in Google Scholar
Chen R.M., Nie X.L., Qin Y.X., Dong G.Q. & Sun Q.X. 2005. Isolation and characterization of genes encoding Myb transcription factor in wheat (Triticum aestivum L.). Plant Sci. 169: 1146–1154.10.1016/j.plantsci.2005.07.018Suche in Google Scholar
Cheng C., Yun K.Y., Ressom H.W., Mohanty B., Bajic V.B., Jia Y.L., Yun S.J. & Reyes B.G. 2007. An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice. BMC Genomics 8: 175.10.1186/1471-2164-8-175Suche in Google Scholar PubMed PubMed Central
Czechowski T., Bari R.P., Stitt M., Scheible W.R. & Udvardi M.K. 2004. Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root- and shoot-specific genes. Plant J. 38: 366– 379.10.1111/j.1365-313X.2004.02051.xSuche in Google Scholar PubMed
Deluc L., Barrieu F., Marchive C., Lauvergeat V., Decendit A., Richard T., Carde J.P., Merillon J.M. & Hamdi S. 2006. Characterization of a grapevine R2R3-Myb transcription factor that regulates the phenylpropanoid pathway. Plant Physiol. 140: 499–511.10.1104/pp.105.067231Suche in Google Scholar PubMed PubMed Central
Dewey D.R. 1984. The genomic system of classification as a guide to intergeneric hybridization with the perennial Triticeae, pp. 209–279. In: Gustafson J.P. (ed.), Gene Manipulation in Plant Improvement. Plenem Press, New York.10.1007/978-1-4613-2429-4_9Suche in Google Scholar
Dias A.P., Braun E.L., McMullen M. & Grotewold E. 2003. Recently duplicated maize R2R3 Myb genes provide evidence for distinct mechanisms of evolutionary divergence after duplication. Plant Physiol. 131: 610–620.10.1104/pp.012047Suche in Google Scholar PubMed PubMed Central
Du H., Feng B.R., Yang S.S., Huang Y.B. & Tang Y.X. 2012. The R2R3-MYB transcription factor gene family in maize. PLoS One 7(6): e37463.10.1371/journal.pone.0037463Suche in Google Scholar PubMed PubMed Central
Durbarry A., Vizir I. & Twell D. 2005. Male Germ Line Development in Arabidopsis. duo pollen mutants reveal gametophytic regulators of generative cell cycle progression. Plant Physiol. 137 297–307.10.1104/pp.104.053165Suche in Google Scholar PubMed PubMed Central
Felsenstein J. 1985. Confidence limits on phylogeny: an approach using the bootstrap. Evolution 39: 783–791.10.1111/j.1558-5646.1985.tb00420.xSuche in Google Scholar PubMed
Glover B.J., Perez-Rodriguez M. & Martin C. 1998. Development of several epidermal cell types can be specified by the same Myb-related plant transcription factor. Development 125: 3497–3508.10.1242/dev.125.17.3497Suche in Google Scholar PubMed
Heine J.G.F. 2006. Functional analysis of P1, a model R2R3-Myb domain transcription factor. The Ohio State University.Suche in Google Scholar
Jiang C., Gu X. & Peterson T. 2004. Identification of conserved gene structures and carboxy-terminal motifs in the Myb gene family of Arabidopsis and Oryza sativa L. ssp. indica. Genome Biol. 5: R46.10.1186/gb-2004-5-7-r46Suche in Google Scholar PubMed PubMed Central
Jia D.S., Mao X.G., Wu R.L., Zhang X.K., et al. 2008. Cloning and expression of transcription factor TaMyb2s in wheat. Acta Agron. Sin. 34: 1323–1329.10.3724/SP.J.1006.2008.01323Suche in Google Scholar
Jin H. & Martin C. 1999. Multifunctionality and diversity within the plant Myb-gene family. Plant Mol. Biol. 41: 577–585.10.1023/A:1006319732410Suche in Google Scholar
Katiyar A., Smita S., Lenka S.K., Rajwanshi R., Chinnusamy V. & Bansal K.C. 2012. Genome-wide classification and expression analysis of Myb transcription factor families in rice and Arabidopsis. BMC Genomics 13: 544.10.1186/1471-2164-13-544Suche in Google Scholar
Kirik V., Schnittger A., Radchuk V., Adler K., Hulskamp M. & Baumlein H. 2001. Ectopic expression of the Arabidopsis AtMYB23 gene induces differentiation of trichome cells. Develop. Biol. 235: 366–377.10.1006/dbio.2001.0287Suche in Google Scholar
Kobayashy S., Ishimaru M., Hiraoka K. & Honda C. 2002. Myb-related genes of the Kyoho grape (Vitis labruscana) regulate anthocyanin biosynthesis. Planta 215: 924–933.10.1007/s00425-002-0830-5Suche in Google Scholar
Kranz H.D., Denekamp M., Greco R., Jin H., Leyva A., Meissner R.C., Petroni K., Urzainqui A., Bevan M., Martin C., Smeekens S., Tonelli C., Paz-Ares J. & Weisshaar B. 1998. Towards functional characterization of the members of the R2R3-Myb gene family from Arabidopsis thaliana. Plant J. 16: 263–276.10.1046/j.1365-313x.1998.00278.xSuche in Google Scholar
Lee M.M. & Schiefelbein J. 1999. WEREWOLF, a Myb-related protein in Arabidopsis, is a position-dependent regulator of epidermal cell patterning. Cell 99: 473–483.10.1016/S0092-8674(00)81536-6Suche in Google Scholar
Loguerico L.L., Zhang J.Q. & Wilkins T.A. 1999. Differential regulation of six novel Myb-domain genes defines two distinct expression patterns in allotetraploid cotton (Gossypium hirsutum L.). Mol. Gen. Genet. 261: 660–671.10.1007/s004380050009Suche in Google Scholar
Ma L., Sun N., Liu X.G., Jiao Y.L., Zhao H.Y. & Deng X.W. 2005. Organ-specific expression of Arabidopsis genome during development. Plant Physiol. 138: 80–91.10.1104/pp.104.054783Suche in Google Scholar
Mandaokar A., Thines B., Shin B., Lange B.M., Choi G., Koo Y.J., Yoo Y.J., Choi Y.D., Choi G. & Browse J. 2006. Transcriptional regulators of stamen development in Arabidopsis identified by transcriptional profiling. Plant J. 46: 984–1008.10.1111/j.1365-313X.2006.02756.xSuche in Google Scholar
Martin C. & Paz-Ares J. 1997. Myb transcription factors in plants. Trends Genet. 13: 67–73.10.1016/S0168-9525(96)10049-4Suche in Google Scholar
Noda K., Glover B.J., Linstead P. & Martin C. 1994. Flower color intensity depends on specialized cell shape controlled by a Myb-related transcription factor. Nature 369: 661–664.10.1038/369661a0Suche in Google Scholar PubMed
Omer S., Kumar S. & Khan B.M. 2013. Over-expression of a subgroup 4 R2R3 type Myb transcription factor gene from Leucaena leucocephala reduces lignin content in transgenic tobacco. Plant Cell Rep. 32: 161–171.10.1007/s00299-012-1350-9Suche in Google Scholar PubMed
Payne T., Clement J., Arnold D. & Lloyd A. 1999. Heterologous Myb genes distinct from GL1 enhance trichome production when overexpressed in Nicotiana tabacum. Development 126: 671–682.10.1242/dev.126.4.671Suche in Google Scholar PubMed
Paz-Ares J., Ghosal D., Wienand U., Peterson P.A. & Saedler H. 1987. The regulatory c1 locus of Zea mays encodes a protein with homology to Myb proto-oncogene products and with structural similarities to transcriptional activators. EMBO J. 6: 3553–3558.10.1002/j.1460-2075.1987.tb02684.xSuche in Google Scholar PubMed PubMed Central
Rabinowicz P.D., Braun E.L., Wolfe A.D., Bowen B. & Grotewold E. 1999. Maize R2R3-Myb genes: sequence analysis reveals amplification in the higher plants. Genetics 153 427–444.10.1093/genetics/153.1.427Suche in Google Scholar PubMed PubMed Central
Reichmann J.L., Heard J., Martin G., Reuber G., Jiang C.Z., Keddie J., Adam L., Pineda O., Ratcliffe O.J., Samaha R.R., Creelman R., Pilgrim M., Broun P., Zhang J.Z., Ghandehari D., Sherman B.K. & Yu G.L. 2000. Arabidopsis transcription factors: Genome-wide comparative analysis among eukaryotes. Science 290: 2105–2110.10.1081/E-EPCS-120010627Suche in Google Scholar
Romero A., Fuertes M.J., Benito J.M., Malpica A., Leyva, J. Paz-Ares. 1998. More than 80R2R3-MYB regulatory genes in the genome of Arabidopsis thaliana. Plant J. 14: 273–28410.1046/j.1365-313X.1998.00113.xSuche in Google Scholar
Saghai-Maroof M.A., Soliman K.M., Jorgensen R.A. & Allard R.W. 1984. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc. Natl. Acad. Sci. USA 81: 8014– 8018.10.1073/pnas.81.24.8014Suche in Google Scholar PubMed PubMed Central
Schmitz G., Tillmann E., Carriero F., Fiore C., Cellini F. & Theres K. 2002. The tomato Blind gene encodes a Myb transcription factor that controls the formation of lateral meristems. Proc. Natl. Acad. Sci. USA 99: 1064–1069.10.1073/pnas.022516199Suche in Google Scholar PubMed PubMed Central
Schmid M., Davson T.S., Henz S.R., Pape U.J., Demar M., Vingron M., Scholkopf B., Weigel D. & Lohmann J.U. 2005. A gene expression map of Arabidopsis thaliana development. Natural Genetics 37: 501–506.10.1038/ng1543Suche in Google Scholar PubMed
Silvia F., Sonbol F.M., Maes T., Capellades M., Puigdomenech P., Rigau J. & Caparros-Ruiz D. 2006. Down-regulation of the maize and Arabidopsis thaliana caffeic acid O-methyl-transferase genes by two new maize R2R3-Myb transcription factors. Plant Mol. Biol. 62: 809–823.10.1007/s11103-006-9058-2Suche in Google Scholar PubMed
Stracke R., Werber M. & Weisshaar B. 2001. The R2R3-MYB gene family in Arabidopsis thaliana. Curr. Opin. Plant Biol. 4: 447–456.10.1016/S1369-5266(00)00199-0Suche in Google Scholar
Stracke R., Ishihara H., Huep G., Barsch A., Mehrtens F., Niehaus K. & Weisshaar B. 2007. Differential regulation of closely related R2R3-Myb transcription factors controls flavonol accumulation in different parts of the Arabidopsis thaliana seedling. Plant J. 50: 660–677.10.1111/j.1365-313X.2007.03078.xSuche in Google Scholar
Tamura K., Dudley J., Nei M. & Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24: 1596–1599.10.1093/molbev/msm092Suche in Google Scholar
Timmermans M.C., Hudson A, Becraft P.W., Nelson T. 1999. ROUGH SHEATH2: a Myb protein that represses knox homeobox genes in maize lateral organ primordia. Science 284: 151–153.10.1126/science.284.5411.151Suche in Google Scholar
Tzvelev N.N. 1976. Zlaki SSSR (Poaceae USSR). Leningrad Rus. Fed.: Nauka.Suche in Google Scholar
Verdonk J.C., Haring M.A., van Tunen A.J & Schuurink R.C. 2005. ODORANT1 regulates flagrance biosynthesis in petunia flowers. Plant Cell 17: 1612–1624.10.1105/tpc.104.028837Suche in Google Scholar
Waites R., Selvadurai H.R., Oliver I.R. & Hudson A. 1998. The PHANTASTICA gene encodes a Myb transcription factor involved in growth and dorsoventrality of lateral organs in Antirrhinum. Cell 93: 779–789.10.1016/S0092-8674(00)81439-7Suche in Google Scholar
Wang G., Wang H., Zhu J., Zhang X.W., Wang F., Tang Y.P., Mei B., Xu Z.K. & Song R. 2010. An expression analysis of 57 transcription factors derived from ESTs of developing seeds in maize (Zea mays). Plant Cell Rep. 29: 545–559.10.1007/s00299-010-0843-7Suche in Google Scholar PubMed
Wissenbach M., Überlacker B., Vogt F., Becker D., Salamini F. & Rohde W. 1993. Myb genes from Hordeum vulgare: tissue-specific expression of chimeric Myb promoter/Gus genes in transgenic tobacco. Plant J. 4: 411–422.10.1046/j.1365-313X.1993.04030411.xSuche in Google Scholar
Wu X.M., Lim S.H. & Yang W.C. 2003. Characterization expression and phylogenetic study of R2R3-Myb genes in orchid. Plant Mol. Biol. 51: 959–972.10.1023/A:1023050110077Suche in Google Scholar
Xue B., Charest P.J., Devantier Y. & Rutledge R.G. 2003. Characterization of a Myb-R2R3 gene from black spruce (Picea mariana) that shares functional conservation with maize C1. Mol. Gen. Genom. 270: 78–86.10.1007/s00438-003-0898-zSuche in Google Scholar PubMed
Yang R.W., Zhou Y.H., Ding C.B., Zheng Y.L. & Zhang L. 2008. Relationships among Leymus species assessed by RAPD markers. Biol. Plant. 52: 237–241.10.1007/s10535-008-0052-1Suche in Google Scholar
Yang R.W., Zhou Y.H., Zhang Y., Zheng Y.L. & Ding C.B. 2006. The genetic diversity among Leymus species based on random amplified microsatellite polymorphism (RAMP). Genet. Resour. Crop Evol. 53: 139–144.10.1007/s10722-004-1933-6Suche in Google Scholar
Yuan Y.W., Sagawa J.M., Di Stilio V.S. & Bradshaw H.D. 2013. Bulk segregant analysis of an induced floral mutant identifies a MIXTA-like R2R3-Myb controlling nectar guide formation in Mimulus lewisii. Genetics 194: 523–528.10.1534/genetics.113.151225Suche in Google Scholar PubMed PubMed Central
Yun K.Y., Park M.R., Mohanty B., Herath V., Xu F., Mauleon R., Wijaya E., Bajic V.B., Bruskiewich R. & Reyes B.G. 2010. Transcriptional regulatory network triggered by oxidative signals configures the early response mechanisms of Japonica rice to chilling stress. BMC Plant Biol. 10: 16.10.1186/1471-2229-10-16Suche in Google Scholar PubMed PubMed Central
Zhao K. & Bartley L.E. 2014. Comparative genomic analysis of the R2R3 MYB secondary cell wall regulators of Arabidopsis, poplar, rice, maize, and switchgrass. BMC Plant Biol. 14: 135.10.1186/1471-2229-14-135Suche in Google Scholar PubMed PubMed Central
Zhang L.C., Zhao G.Y., Jia J.Z., Liu X. & Kong X.Y. 2012. Molecular characterization of 60 isolated wheat Myb genes and analysis of their expression during abiotic stress. J. Exp. Bot. 63: 203–214.10.1093/jxb/err264Suche in Google Scholar PubMed PubMed Central
Zimmermann I.M., Heim M.A., Weisshaar B. & Uhrig J.F. 2004. Comprehensive identification of Arabidopsis thaliana Myb transcription factors interacting with R/B-like BHLH proteins. Plant J. 40: 22–34.10.1111/j.1365-313X.2004.02183.xSuche in Google Scholar PubMed
© 2016 Institute of Botany, Slovak Academy of Sciences
Artikel in diesem Heft
- Section Cellular and Molecular Biology
- Pigments from fungi, an opportunity of production for diverse applications
- Section Zoology
- Morphological view on the evolution of the immunity and lymphoid organs of vertebrates, focused on thymus
- Section Cellular and Molecular Biology
- p53-Fibrinolytic system and acute lung injury
- Section Cellular and Molecular Biology
- The multipotent action of electromagnetic field
- Section Cellular and Molecular Biology
- Prescreening, identification and harvesting of microalgae with antibacterial activity
- Section Botany
- Cloning and molecular characterization of Myb transcription factors from Leymus (Poaceae: Trticeae)
- Section Botany
- Hydrological soil behavior in areas with semi-arid vegetation (Beni Chougrane Mountains, Algeria)
- Section Botany
- Extent and persistence of water repellency in two Iranian soils
- Section Botany
- The impact of the permanent grass cover or conventional tillage on hydraulic properties of Haplic Cambisol developed on paragneiss substrate
- Section Zoology
- Species composition of tetranychoid mites (Acari: Trombidiformes: Prostigmata: Tetranychoidea) in main landscapes of Tehran and modelling ecological niche of Tetranychoidea in main climates of Tehran Province, Iran
- Section Zoology
- Abiotic factors affect the occurrence of different morphological characteristics in Erebia medusa (Lepidoptera: Nymphalidae)
- Section Zoology
- Comparison of molecular and morphometric analysis in species discrimination of larvae among five cyprinids from the subfamily Leuciscinae: A tool for sustainable conservation of riverine ichthyofauna
- Section Zoology
- Values of three branched plasma amino acids of farmed rainbow trout, Oncorhynchus mykiss
Artikel in diesem Heft
- Section Cellular and Molecular Biology
- Pigments from fungi, an opportunity of production for diverse applications
- Section Zoology
- Morphological view on the evolution of the immunity and lymphoid organs of vertebrates, focused on thymus
- Section Cellular and Molecular Biology
- p53-Fibrinolytic system and acute lung injury
- Section Cellular and Molecular Biology
- The multipotent action of electromagnetic field
- Section Cellular and Molecular Biology
- Prescreening, identification and harvesting of microalgae with antibacterial activity
- Section Botany
- Cloning and molecular characterization of Myb transcription factors from Leymus (Poaceae: Trticeae)
- Section Botany
- Hydrological soil behavior in areas with semi-arid vegetation (Beni Chougrane Mountains, Algeria)
- Section Botany
- Extent and persistence of water repellency in two Iranian soils
- Section Botany
- The impact of the permanent grass cover or conventional tillage on hydraulic properties of Haplic Cambisol developed on paragneiss substrate
- Section Zoology
- Species composition of tetranychoid mites (Acari: Trombidiformes: Prostigmata: Tetranychoidea) in main landscapes of Tehran and modelling ecological niche of Tetranychoidea in main climates of Tehran Province, Iran
- Section Zoology
- Abiotic factors affect the occurrence of different morphological characteristics in Erebia medusa (Lepidoptera: Nymphalidae)
- Section Zoology
- Comparison of molecular and morphometric analysis in species discrimination of larvae among five cyprinids from the subfamily Leuciscinae: A tool for sustainable conservation of riverine ichthyofauna
- Section Zoology
- Values of three branched plasma amino acids of farmed rainbow trout, Oncorhynchus mykiss