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A new automated human leukocyte antigen genotyping strategy to identify DR-DQ risk alleles for celiac disease and type 1 diabetes mellitus
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Ewa H. Lavant
Published/Copyright:
December 1, 2009
Abstract
Background: The risk for type 1 diabetes mellitus (T1DM) and celiac disease (CD) is related to human leukocyte antigen (HLA) DQA1, DQB1 and DRB1 loci. Unfortunately, HLA typing has been too difficult and costly for frequent use. Automated genotyping focused on risk alleles could provide access to HLA typing in diagnostic evaluations, epidemiological screening and contribute to preventive strategies.
Methods: A sequence specific primer amplification method requiring a total of four PCR reactions, one restriction enzyme digestion and a single electrophoretic step provides low to medium resolution typing of DQA1, DQB1 and DRB1 using Applied Biosystems 3730 DNA analyzer. The method was validated using 261 samples with genotypes determined using a reference method.
Results: Specific fluorescent DQA1, DQB1 and DRB1 amplicons were of expected size. Concordance with the reference method was 100% for DQA1 and DQB1 alleles and 99.8% for DRB1 alleles.
Conclusions: We have developed a high throughput HLA typing method that accurately distinguishes risk alleles for T1DM and CD. This method allows screening of several thousand samples per week, consuming 32 ng of DNA template, low reagent volumes and minimal time for data review.
Clin Chem Lab Med 2009;47:1489–95.
Keywords: automation; celiac disease; HLA-DR-DQ-genotyping; PCR-SSP (sequence specific priming); type 1 diabetes mellitus
Received: 2009-6-18
Accepted: 2009-9-1
Published Online: 2009-12-01
Published in Print: 2009-12-01
©2009 by Walter de Gruyter Berlin New York
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Keywords for this article
automation;
celiac disease;
HLA-DR-DQ-genotyping;
PCR-SSP (sequence specific priming);
type 1 diabetes mellitus
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- Multiple gene interaction and modulation of hemostatic balance
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