Article
Licensed
Unlicensed
Requires Authentication
Aging and endothelial progenitor cell telomere length in healthy men
-
Erich J. Kushner
Published/Copyright:
December 5, 2008
Abstract
Background: Telomere length declines with age in mature endothelial cells and is thought to contribute to endothelial dysfunction and atherogenesis. Bone marrow-derived circulating endothelial progenitor cells (EPCs) are critical to vascular health as they contribute to both reendothelialization and neovascularization. We tested the hypothesis that EPC telomere length decreases with age in healthy adult humans.
Methods: Peripheral blood samples were collected from 40 healthy, non-obese, sedentary men: 12 young (age 21–34 years), 12 middle-aged (43–55 years) and 16 older (57–68 years). Putative EPCs were isolated from peripheral blood mononuclear cells and telomere length was determined using genomic DNA preparation and Southern hybridization techniques.
Results: EPC telomere length (base pairs) was ∼20% (p=0.01) lower in the older (8492+523 bp) compared to the middle-aged (10,565+572 bp) and young (10,205+501 bp) men. Of note, there was no difference in EPC telomere length between the middle-aged and young men.
Conclusions: These results demonstrate that EPC telomere length declines with age in healthy, sedentary men. Interestingly, telomere length was well preserved in the middle-aged compared to young men, suggesting that EPC telomere shortening occurs after the age of 55 years.
Clin Chem Lab Med 2009;47:47–50.
Received: 2008-7-7
Accepted: 2008-8-26
Published Online: 2008-12-05
Published Online: 2008-12-5
Published in Print: 2009-01-01
©2009 by Walter de Gruyter Berlin New York
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Reviews
- Molecular markers detecting circulating melanoma cells by reverse transcription polymerase chain reaction: methodological pitfalls and clinical relevance
- HIV-GB virus C co-infection: an overview
- Genetics and Molecular Diagnostics
- Relationships between C-reactive protein concentration and genotype in healthy volunteers
- SYBR Green-based real-time PCR assay for detection of VKORC1 and CYP2C9 polymorphisms that modulate warfarin dose requirement
- Functional polymorphisms in ACE and CYP11B2 genes and atrial fibrillation in patients with hypertensive heart disease
- No effect of MDR1 C3435T polymorphism on oral pharmacokinetics of telmisartan in 19 healthy Chinese male subjects
- A novel large deletion and three polymorphisms in the FECH gene associated with erythropoietic protoporphyria
- Aging and endothelial progenitor cell telomere length in healthy men
- Gln222Arg (A2317G) polymorphism in the deoxyribonuclease I gene exhibits ethnic and functional differences
- Mutation screening of the medium-chain acyl-CoA dehydrogenase (MCAD) and the ornithine transcarbamylase (OTC) genes by multiplex PCR amplification and sequencing
- General Clinical Chemistry and Laboratory Medicine
- Nitrated apolipoprotein A-I, a potential new cardiovascular marker, is markedly increased in low high-density lipoprotein cholesterol subjects
- Relationships of serum haptoglobin concentration with HbA1c and glycated albumin concentrations in Japanese type 2 diabetic patients
- Multi-elements (aluminium, copper, magnesium, manganese, selenium and zinc) determination in serum by dynamic reaction cell-inductively coupled plasma-mass spectrometry
- Urinary neutrophil gelatinase-associated lipocalin (NGAL) as an early marker of acute kidney injury in critically ill multiple trauma patients
- Validation and Outcome Studies
- Validation for quantification of immunoglobulins by Fourier transform infrared spectrometry
- Albumin-based or albumin-linked calibrators cause a positive bias in serum proteins assayed by the biuret method
- Current practices in antinuclear antibody testing: results from the Belgian External Quality Assessment Scheme
- Verification of the analytical range of a new reagent for full-range C-reactive protein determination
- Measurement uncertainty in manual differential leukocyte counting
- Letters to the Editor
- Falsely elevated carbohydrate antigen 19-9 level due to heterophilic antibody interference but not rheumatoid factor: a case report
- An unusual interference in parathormone assay caused by anti-goat IgG: a case report
Articles in the same Issue
- Reviews
- Molecular markers detecting circulating melanoma cells by reverse transcription polymerase chain reaction: methodological pitfalls and clinical relevance
- HIV-GB virus C co-infection: an overview
- Genetics and Molecular Diagnostics
- Relationships between C-reactive protein concentration and genotype in healthy volunteers
- SYBR Green-based real-time PCR assay for detection of VKORC1 and CYP2C9 polymorphisms that modulate warfarin dose requirement
- Functional polymorphisms in ACE and CYP11B2 genes and atrial fibrillation in patients with hypertensive heart disease
- No effect of MDR1 C3435T polymorphism on oral pharmacokinetics of telmisartan in 19 healthy Chinese male subjects
- A novel large deletion and three polymorphisms in the FECH gene associated with erythropoietic protoporphyria
- Aging and endothelial progenitor cell telomere length in healthy men
- Gln222Arg (A2317G) polymorphism in the deoxyribonuclease I gene exhibits ethnic and functional differences
- Mutation screening of the medium-chain acyl-CoA dehydrogenase (MCAD) and the ornithine transcarbamylase (OTC) genes by multiplex PCR amplification and sequencing
- General Clinical Chemistry and Laboratory Medicine
- Nitrated apolipoprotein A-I, a potential new cardiovascular marker, is markedly increased in low high-density lipoprotein cholesterol subjects
- Relationships of serum haptoglobin concentration with HbA1c and glycated albumin concentrations in Japanese type 2 diabetic patients
- Multi-elements (aluminium, copper, magnesium, manganese, selenium and zinc) determination in serum by dynamic reaction cell-inductively coupled plasma-mass spectrometry
- Urinary neutrophil gelatinase-associated lipocalin (NGAL) as an early marker of acute kidney injury in critically ill multiple trauma patients
- Validation and Outcome Studies
- Validation for quantification of immunoglobulins by Fourier transform infrared spectrometry
- Albumin-based or albumin-linked calibrators cause a positive bias in serum proteins assayed by the biuret method
- Current practices in antinuclear antibody testing: results from the Belgian External Quality Assessment Scheme
- Verification of the analytical range of a new reagent for full-range C-reactive protein determination
- Measurement uncertainty in manual differential leukocyte counting
- Letters to the Editor
- Falsely elevated carbohydrate antigen 19-9 level due to heterophilic antibody interference but not rheumatoid factor: a case report
- An unusual interference in parathormone assay caused by anti-goat IgG: a case report
