Article
Licensed
Unlicensed
Requires Authentication
Validation for quantification of immunoglobulins by Fourier transform infrared spectrometry
-
Lamia Benezzeddine-Boussaidi
Published/Copyright:
December 31, 2008
Abstract
Background: The objective of this study was to develop a robust quantification method for simultaneously analyzing molecules in human plasma using the Fourier transform infrared (FT-IR) system with a partial least square (PLS) regression.
Methods: Plasma spectra were analyzed from 4000 to 500 cm−1 (with 2.0 cm−1 of resolution and 32 scans), and the molecule concentrations (IgA, IgG, IgM) were measured blindly by using a cross-validation model prepared by PLS analysis of data from 135 samples.
Results: There was a significant correlation between the FT-IR predicted concentration and the concentration obtained with the clinical reference method: R2=0.98 (IgA), R2=0.98 (IgG), and R2=0.97 (IgM). The root mean square error of prediction (RMSEP) was 0.05 g·L−1 (IgA), 0.4 g·L−1 (IgG), and 0.03 g·L−1 (IgM). Variability of inter-experimenter reproducibility was less than 2%. The interchangeability of the two methods was studied by using the Bland-Altman method.
Conclusions: Together with PLS analysis, FT-IR spectrometry appears to be an easy-to-use and accurate method to determine multianalyte concentrations in dried human plasma. It could be an alternative tool for rapidly quantifying many molecules after developing a specific predictive model.
Clin Chem Lab Med 2009;47:83–90.
Keywords: immunoglobulins; multivariate analysis; plasma molecules; Fourier transform infrared (FT-IR) spectrometry; validation
Received: 2008-4-30
Accepted: 2008-9-1
Published Online: 2008-12-31
Published in Print: 2009-01-01
©2009 by Walter de Gruyter Berlin New York
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Reviews
- Molecular markers detecting circulating melanoma cells by reverse transcription polymerase chain reaction: methodological pitfalls and clinical relevance
- HIV-GB virus C co-infection: an overview
- Genetics and Molecular Diagnostics
- Relationships between C-reactive protein concentration and genotype in healthy volunteers
- SYBR Green-based real-time PCR assay for detection of VKORC1 and CYP2C9 polymorphisms that modulate warfarin dose requirement
- Functional polymorphisms in ACE and CYP11B2 genes and atrial fibrillation in patients with hypertensive heart disease
- No effect of MDR1 C3435T polymorphism on oral pharmacokinetics of telmisartan in 19 healthy Chinese male subjects
- A novel large deletion and three polymorphisms in the FECH gene associated with erythropoietic protoporphyria
- Aging and endothelial progenitor cell telomere length in healthy men
- Gln222Arg (A2317G) polymorphism in the deoxyribonuclease I gene exhibits ethnic and functional differences
- Mutation screening of the medium-chain acyl-CoA dehydrogenase (MCAD) and the ornithine transcarbamylase (OTC) genes by multiplex PCR amplification and sequencing
- General Clinical Chemistry and Laboratory Medicine
- Nitrated apolipoprotein A-I, a potential new cardiovascular marker, is markedly increased in low high-density lipoprotein cholesterol subjects
- Relationships of serum haptoglobin concentration with HbA1c and glycated albumin concentrations in Japanese type 2 diabetic patients
- Multi-elements (aluminium, copper, magnesium, manganese, selenium and zinc) determination in serum by dynamic reaction cell-inductively coupled plasma-mass spectrometry
- Urinary neutrophil gelatinase-associated lipocalin (NGAL) as an early marker of acute kidney injury in critically ill multiple trauma patients
- Validation and Outcome Studies
- Validation for quantification of immunoglobulins by Fourier transform infrared spectrometry
- Albumin-based or albumin-linked calibrators cause a positive bias in serum proteins assayed by the biuret method
- Current practices in antinuclear antibody testing: results from the Belgian External Quality Assessment Scheme
- Verification of the analytical range of a new reagent for full-range C-reactive protein determination
- Measurement uncertainty in manual differential leukocyte counting
- Letters to the Editor
- Falsely elevated carbohydrate antigen 19-9 level due to heterophilic antibody interference but not rheumatoid factor: a case report
- An unusual interference in parathormone assay caused by anti-goat IgG: a case report
Keywords for this article
immunoglobulins;
multivariate analysis;
plasma molecules;
Fourier transform infrared (FT-IR) spectrometry;
validation
Articles in the same Issue
- Reviews
- Molecular markers detecting circulating melanoma cells by reverse transcription polymerase chain reaction: methodological pitfalls and clinical relevance
- HIV-GB virus C co-infection: an overview
- Genetics and Molecular Diagnostics
- Relationships between C-reactive protein concentration and genotype in healthy volunteers
- SYBR Green-based real-time PCR assay for detection of VKORC1 and CYP2C9 polymorphisms that modulate warfarin dose requirement
- Functional polymorphisms in ACE and CYP11B2 genes and atrial fibrillation in patients with hypertensive heart disease
- No effect of MDR1 C3435T polymorphism on oral pharmacokinetics of telmisartan in 19 healthy Chinese male subjects
- A novel large deletion and three polymorphisms in the FECH gene associated with erythropoietic protoporphyria
- Aging and endothelial progenitor cell telomere length in healthy men
- Gln222Arg (A2317G) polymorphism in the deoxyribonuclease I gene exhibits ethnic and functional differences
- Mutation screening of the medium-chain acyl-CoA dehydrogenase (MCAD) and the ornithine transcarbamylase (OTC) genes by multiplex PCR amplification and sequencing
- General Clinical Chemistry and Laboratory Medicine
- Nitrated apolipoprotein A-I, a potential new cardiovascular marker, is markedly increased in low high-density lipoprotein cholesterol subjects
- Relationships of serum haptoglobin concentration with HbA1c and glycated albumin concentrations in Japanese type 2 diabetic patients
- Multi-elements (aluminium, copper, magnesium, manganese, selenium and zinc) determination in serum by dynamic reaction cell-inductively coupled plasma-mass spectrometry
- Urinary neutrophil gelatinase-associated lipocalin (NGAL) as an early marker of acute kidney injury in critically ill multiple trauma patients
- Validation and Outcome Studies
- Validation for quantification of immunoglobulins by Fourier transform infrared spectrometry
- Albumin-based or albumin-linked calibrators cause a positive bias in serum proteins assayed by the biuret method
- Current practices in antinuclear antibody testing: results from the Belgian External Quality Assessment Scheme
- Verification of the analytical range of a new reagent for full-range C-reactive protein determination
- Measurement uncertainty in manual differential leukocyte counting
- Letters to the Editor
- Falsely elevated carbohydrate antigen 19-9 level due to heterophilic antibody interference but not rheumatoid factor: a case report
- An unusual interference in parathormone assay caused by anti-goat IgG: a case report
