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Drugs and brain death diagnostics: determination of drugs capable of inducing EEG zero line
-
Andreas Meinitzer
Veröffentlicht/Copyright:
31. Oktober 2008
Abstract
Background: Several drugs may affect the diagnosis of brain death by depressing the electroencephalographic signal. Serum levels of these drugs must be below their respective therapeutic ranges.
Methods: A high performance liquid chromatography-based fast and simple method was developed for determination of thiopentone, pentobarbitone, phenobarbitone, methohexital and propofol in serum and validated according to international recommendations.
Results: Separation of extracted analytes was performed on a reversed phase column [Agilent Zorbax SB C18, 5 μm, 4.6×250 mm; mobile phase 50% 50 mM NaH2PO4 pH 4.6 mixed with 35% (v/v) acetonitrile and 15% (v/v) methanol]. Calibration curves were linear throughout the selected ranges (μg/mL, thiopentone 0.25–50, pentobarbitone 0.25–25, phenobarbitone 2.5–50, methohexital 0.125–2.50, propofol 0.25–5.0). The standard deviations for the regression line, recovery, imprecision and accuracy results were all highly satisfactory. The lower limits of quantification for propofol, thiopentone and pentobarbitone were set at 0.25 μg/mL, for phenobarbitone 2.5 μg/mL, and for methohexital 0.125 μg/mL, which are below the lowest pharmacologically relevant serum concentrations. Intra- and inter-day coefficients of variation were less than 10% throughout as determined with six replicates.
Conclusions: The method presented is suitable for drug monitoring to help enhance the reliability of the diagnosis of brain death.
Clin Chem Lab Med 2008;46:1732–8.
Keywords: brain death diagnosis; chromatographic method; drug analysis; electroencephalography; validation
Received: 2008-5-21
Accepted: 2008-8-5
Published Online: 2008-10-31
Published in Print: 2008-12-01
©2008 by Walter de Gruyter Berlin New York
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Schlagwörter für diesen Artikel
brain death diagnosis;
chromatographic method;
drug analysis;
electroencephalography;
validation
Artikel in diesem Heft
- Editors' and Publisher's Note
- CCLM Award for the most cited paper recently published
- Reviews
- Cystatin C: current position and future prospects
- Some heterogeneity factors affecting the B-type natriuretic peptides outcome: a meta-analysis
- Genetics and Molecular Diagnostics
- Effect of cyclin D1 (CCND1) polymorphism on gastric premalignant condition
- Identification of two de novo mutations in Chinese patients with X-linked adrenoleukodystrophy
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