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Five commercially available insulin-like growth factor I (IGF-I) assays in comparison to the former Nichols Advantage IGF-I in a growth hormone treated population
-
Alexander Krebs
Published/Copyright:
December 1, 2008
Abstract
Background: The serum insulin-like growth factor I (IGF-I) level is accepted to diagnose the growth hormone (GH) status. Here, we evaluated the DRG IGF-I 600 ELISA, DSL IGF-I ELISA, IDS OCTEIA IGF-I, Mediagnost IGF-I-ELISA, and the Siemens Immulite 2500 IGF-I in comparison to the former Nichols Advantage IGF-I assay.
Methods: Imprecision was determined by use of a serum pool and commercial control materials. Accuracy was evaluated by means of a method comparison to Nichols in 173 serum samples of GH deficient patients.
Results: The Siemens and the IDS IGF-I assays showed the lowest imprecision with coefficients of variation up to 3.6% and 6.9%, respectively. Both correlated best to Nichols (Siemens: y=0.667X+8.8 μg/L, r=0.950; IDS: y=0.527X+4.6 μg/L, r=0.927) with the lowest dispersion of residuals from a linear equation. The DSL assay had the highest comparability to Nichols (y=1.000X+35.5 μg/L, r=0.864), but with a considerable scattering.
Conclusions: To yield IGF-I determination comparable to the former Nichols IGF-I, either the Siemens or the IDS assay should be applied, and the results should be converted by a linear method transformation. Where a conversion factor is not desired, the DSL assay should be selected.
Clin Chem Lab Med 2008;46:1776–83.
Keywords: assay; evaluation; growth hormone deficiency; insulin-like growth factor I (IGF-I); sensitivity; specificity
Received: 2008-6-25
Accepted: 2008-8-19
Published Online: 2008-12-01
Published in Print: 2008-12-01
©2008 by Walter de Gruyter Berlin New York
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Keywords for this article
assay;
evaluation;
growth hormone deficiency;
insulin-like growth factor I (IGF-I);
sensitivity;
specificity
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- CCLM Award for the most cited paper recently published
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- Genetics and Molecular Diagnostics
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- CYP21A2 gene mutation analysis in Moroccan patients with classic form of 21-hydroxylase deficiency: high regional prevalence of p.Q318X mutation and identification of a novel p.L353R mutation
- Apolipoprotein A5 gene −1131T/C polymorphism is associated with the risk of metabolic syndrome in ethnic Chinese in Taiwan
- Association of GSTM1 and GSTT1 gene polymorphisms with coronary artery disease in relation to tobacco smoking
- Use of the biochip microarray system in detection of myocardial injury caused by radiofrequency catheter ablation
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