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Reliability of oxidative stress biomarkers in hemodialysis patients: a comparative study
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Simonetta Palleschi
Published/Copyright:
September 11, 2007
Abstract
Background: Oxidative stress (OS) is considered to play a major role in the development of end-stage renal disease (ESRD) complications. However, conflicting and inconsistent data have been reported on OS in ESRD patients. Our aim was to investigate the reliability of the most popular non-enzymatic plasma OS biomarkers in ESRD.
Methods: Vitamins A (VitA), E and C (VitC), uric acid, plasma antioxidant and ferric-reducing potential (PAP and PRP), thiols (SH), malondialdehyde (MDA) and lipid hydroperoxides (HPO) were determined before and after dialysis in plasma from 33 ESRD patients on hemodialysis, hemodiafiltration or peritoneal dialysis and 20 control subjects.
Results: In ESRD patients, high PRP and normal PAP values were positively correlated with VitC levels. After dialysis, PRP levels decreased, while unchanged PAP levels correlated positively with high VitA and transiently recovered SH values. All patients showed high levels of both MDA and cholesterol-normalized HPO. However, while the former significantly decreased after dialysis, the latter were unaffected by treatment. Paradoxical correlations of MDA with both VitA and HPO were found.
Conclusions: Plasma PRP and MDA levels may be dramatically affected by both uremia and dialysis; their use in ESRD patients may therefore lead to OS misevaluation and should be avoided. More reliable results can be obtained using physiologically relevant OS functional tests, such as PAP, and early biomarkers of OS damage, such as SH and HPO.
Clin Chem Lab Med 2007;45:1211–8.
Keywords: antioxidants; chronic kidney failure; lipid peroxides; malondialdehyde; oxidative stress; renal dialysis; vitamins
Received: 2007-3-5
Accepted: 2007-5-21
Published Online: 2007-09-11
Published in Print: 2007-09-01
©2007 by Walter de Gruyter Berlin New York
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Keywords for this article
antioxidants;
chronic kidney failure;
lipid peroxides;
malondialdehyde;
oxidative stress;
renal dialysis;
vitamins
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- Third “Biologie Prospective” Santorini Conference
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- Ghrelin variants influence development of body mass index and plasma levels of total cholesterol in dialyzed patients
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- Apolipoprotein A-V gene polymorphisms in subjects with metabolic syndrome
- Prothrombogenic factors and reduced antioxidative defense in children and adolescents with pre-metabolic and metabolic syndrome
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