Article
Licensed
Unlicensed
Requires Authentication
The c.–292C>T promoter polymorphism increases reticulocyte-type 15-lipoxygenase-1 activity and could be atheroprotective
-
Jonas Wittwer
Published/Copyright:
April 17, 2007
Abstract
Background: Reticulocyte-type 15-lipoxygenase-1 (ALOX15) has anti-inflammatory and inflammatory effects and is implicated in the development of asthma, arthritis and atherosclerosis. Previously, we screened the human ALOX15 gene for variations because genetic variability in ALOX15 might influence these diseases. We found a C>T substitution at position c.–292 in the ALOX15 promoter that created a novel binding site for the transcription factor SPI1 and increased ALOX15 mRNA levels in monocytes from c.–292CT heterozygous volunteers.
Methods: To test whether the higher mRNA levels led to higher ALOX15 activity, we performed an activity assay and measured the arachidonic acid metabolite 15(S)-hydroxy-eicosatetraenoic acid [15(S)-HETE] by HPLC analysis. To test whether this polymorphism was associated with coronary artery disease (CAD), we investigated its association in a case-control study involving 498 Caucasians.
Results: The c.–292C>T polymorphism was associated with higher enzyme activity in heterozygous carriers. Intriguingly, this polymorphism also showed a tendency to be protective against atherosclerosis.
Conclusions: These results suggest that increased ALOX15 activity may attenuate inflammation, which could be caused by an increase in 15(S)-HETE and eventually by its metabolites, the lipoxins.
Clin Chem Lab Med 2007;45:487–92.
Keywords: inflammation; lipoxin; 15-lipoxygenase (15-LOX); polymorphism; PU.1; reticulocyte-type 15-lipoxygenase-1 (ALOX15); SFPI1; SPI1; transcription factor
Received: 2006-10-13
Accepted: 2007-1-4
Published Online: 2007-04-17
Published in Print: 2007-04-01
©2007 by Walter de Gruyter Berlin New York
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Electrophoretic separations of cerebrospinal fluid proteins in clinical investigations
- Haemophilia A: molecular insights
- Quality specifications: self pleasure for clinical laboratories or added value for patient management?
- A724A polymorphism of sarco(endo)plasmic reticulum Ca2+-ATPase 2 (SERCA2) in hypertensive patients
- Associations of common polymorphisms in the thymidylate synthase, reduced folate carrier and 5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine monophosphate cyclohydrolase genes with folate and homocysteine levels and venous thrombosis risk
- Pro12Ala polymorphism of the peroxisome proliferator-activated receptor-γ2 (PPARγ-2) gene is associated with greater insulin sensitivity and decreased risk of type 2 diabetes in an Iranian population
- A novel frameshift mutation of the lecithin:cholesterol acyltransferase (LCAT) gene associated with renal failure in familial LCAT deficiency
- The c.–292C>T promoter polymorphism increases reticulocyte-type 15-lipoxygenase-1 activity and could be atheroprotective
- The genetic polymorphisms of β3-adrenergic receptor (AR) Trp64Arg and β2-AR Gln27Glu are associated with obesity in Chinese male hypertensive patients
- Diagnostic performance of combined specific urinary proteins and urinary flow cytometry in urinary tract pathology
- Diagnostic accuracy of the FIDIS multiplex fluorescent microsphere immunodetection system for anti-extractable nuclear antigen (ENA) antibodies in connective tissue diseases
- Fluorimetric quantitation of citalopram and escitalopram in plasma: developing an express method to monitor compliance in clinical trials
- Free serum cortisol: quantification applying equilibrium dialysis or ultrafiltration and an automated immunoassay system
- Percutaneous coronary intervention-induced variations in systemic parameters of inflammation: relationship with the mode of stenting
- Multicentre physiological reference values for the concentration of creatininium in plasma and diagnostic specificity of glomerular filtration rate estimated with the MDRD equation
- Serum levels of ischemia-modified albumin in healthy volunteers after exercise-induced calf-muscle ischemia
- Reference intervals for follicle-stimulating hormone, luteinizing hormone and prolactin in children and young adults on the bioMérieux Mini-Vidas system
- Limits of usual biochemical alcohol markers in cord blood at term: a fetal/maternal population-based study
- Implementing the Stockholm Conference hierarchy of objective quality criteria in a routine laboratory
- The diffusion of innovations theory could help laboratorians in research translation
- Detection of CYP2D6 polymorphisms *9, *10, and *41 using ARMS-PCR and their allelic frequencies in 400 psychiatric patients
- Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: I. Analyte definition and proposal of a candidate reference method
- Measurement of free thyroxine in laboratory medicine – proposal of measurand definition
Keywords for this article
inflammation;
lipoxin;
15-lipoxygenase (15-LOX);
polymorphism;
PU.1;
reticulocyte-type 15-lipoxygenase-1 (ALOX15);
SFPI1;
SPI1;
transcription factor
Articles in the same Issue
- Electrophoretic separations of cerebrospinal fluid proteins in clinical investigations
- Haemophilia A: molecular insights
- Quality specifications: self pleasure for clinical laboratories or added value for patient management?
- A724A polymorphism of sarco(endo)plasmic reticulum Ca2+-ATPase 2 (SERCA2) in hypertensive patients
- Associations of common polymorphisms in the thymidylate synthase, reduced folate carrier and 5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine monophosphate cyclohydrolase genes with folate and homocysteine levels and venous thrombosis risk
- Pro12Ala polymorphism of the peroxisome proliferator-activated receptor-γ2 (PPARγ-2) gene is associated with greater insulin sensitivity and decreased risk of type 2 diabetes in an Iranian population
- A novel frameshift mutation of the lecithin:cholesterol acyltransferase (LCAT) gene associated with renal failure in familial LCAT deficiency
- The c.–292C>T promoter polymorphism increases reticulocyte-type 15-lipoxygenase-1 activity and could be atheroprotective
- The genetic polymorphisms of β3-adrenergic receptor (AR) Trp64Arg and β2-AR Gln27Glu are associated with obesity in Chinese male hypertensive patients
- Diagnostic performance of combined specific urinary proteins and urinary flow cytometry in urinary tract pathology
- Diagnostic accuracy of the FIDIS multiplex fluorescent microsphere immunodetection system for anti-extractable nuclear antigen (ENA) antibodies in connective tissue diseases
- Fluorimetric quantitation of citalopram and escitalopram in plasma: developing an express method to monitor compliance in clinical trials
- Free serum cortisol: quantification applying equilibrium dialysis or ultrafiltration and an automated immunoassay system
- Percutaneous coronary intervention-induced variations in systemic parameters of inflammation: relationship with the mode of stenting
- Multicentre physiological reference values for the concentration of creatininium in plasma and diagnostic specificity of glomerular filtration rate estimated with the MDRD equation
- Serum levels of ischemia-modified albumin in healthy volunteers after exercise-induced calf-muscle ischemia
- Reference intervals for follicle-stimulating hormone, luteinizing hormone and prolactin in children and young adults on the bioMérieux Mini-Vidas system
- Limits of usual biochemical alcohol markers in cord blood at term: a fetal/maternal population-based study
- Implementing the Stockholm Conference hierarchy of objective quality criteria in a routine laboratory
- The diffusion of innovations theory could help laboratorians in research translation
- Detection of CYP2D6 polymorphisms *9, *10, and *41 using ARMS-PCR and their allelic frequencies in 400 psychiatric patients
- Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: I. Analyte definition and proposal of a candidate reference method
- Measurement of free thyroxine in laboratory medicine – proposal of measurand definition
