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Diagnostic performance of combined specific urinary proteins and urinary flow cytometry in urinary tract pathology
-
Joris Penders
Published/Copyright:
April 17, 2007
Abstract
Background: Urinalysis comprises three techniques: urinary flow cytometry, test strip analysis and determination of specific urinary proteins. We investigated the diagnostic possibilities of combining these methods for a cohort of patients with a variety of well-documented urological and nephrological pathology.
Methods: Urinary samples from 407 in- and out-patients with nephrological or urological pathology were retrospectively included in our study. Test strip analysis (URISYS 2400), urinary flow cytometry (UF-100) and urinary protein analysis [albumin, total protein, α1-microglobulin (A1M), α2-macroglobulin (A2M)] were performed.
Results: In discriminating upper and lower urinary tract infections, A1M and A1M/log(white blood cells) can be used, whereas pathological casts only give poor discrimination. The ratio A2M/log(red blood cells; RBC) allows differentiation between cystitis and pyelonephritis, while glomerular diseases can be recognised by the log(RBC×urinary total protein). Combining A2M and urinary albumin allows the determination of acute prostatitis.
Conclusions: Using combined parameters provided by various urinalysis techniques, ratios can be proposed with diagnostic value for classifying renal and urological conditions. Possible integration in computer-based knowledge systems may offer valuable information for clinicians dealing with these pathologies.
Clin Chem Lab Med 2007;45:499–504.
Keywords: α1-microglobulin; α2-macroglobulin; proteinuria; renal disease; tubulus; urinary tract infection
Received: 2006-10-12
Accepted: 2007-1-4
Published Online: 2007-04-17
Published in Print: 2007-04-01
©2007 by Walter de Gruyter Berlin New York
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Keywords for this article
α1-microglobulin;
α2-macroglobulin;
proteinuria;
renal disease;
tubulus;
urinary tract infection
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