Clinical Evaluation of a New Automated Anti-dsDNA Fluorescent Immunoassay
-
Monserrat Hernando
Abstract
The measurement of anti-double-stranded DNA (antidsDNA) antibodies is a useful tool for the diagnosis and the follow-up of systemic lupus erythematosus (SLE). Anti-dsDNA antibodies are involved in the pathogenesis of lupus nephritis and they are, specially the highavidity antibodies, the most specific antibodies associated with SLE nephritis and active SLE. The aim of the present study was to assess the clinical utility of an enzyme-linked immunosorbent assay (ELISA) that utilizes a circular double-stranded plasmid DNA as a nucleic acid source, adapted to an automated fluorescence immunoassay (EliA™ dsDNA, Pharmacia, Freiburg, Germany). Also, we compared this method with other immunoassays used in clinical laboratories. We have measured anti-dsDNA antibodies in the serum of 179 patients with a positive result for antinuclear antibodies (ANA). Seventy six sera were from SLE patients (14men and 62 women), and the other 103 sera (from 20 men and 83 women) constituted the control group. This latter group includes nine Sjögren's syndrome patients, six patients with rheumatoid arthritis and 88 with various other diseases, including connective tissue diseases (n=34), hepatopathies (n=17; 11 primary biliary cirrhosis and 6 autoimmune hepatitis), and 37 patients with nonautoimmune diseases (viral hepatitis, renal disease, diabetes, exanthema and hypertension). Methods used were “EliA™ dsDNA” (Pharmacia, Germany), “Varelisa® dsDNA” (Pharmacia, Germany), Farr (Amersham, UK) and Chritidia luciliae immunofluorescence test (Vitro-Immun, Germany). We assessed sensitivity, specificity, positive predictive value and negative predictive value in the clinical study, and kappa index and scatter plots in the comparative study. The results show a low concordance between methods (κ<0.6). The evaluated EliA method shows a very good specificity for SLE (93.2%) and a good sensitivity for active SLE (70.8%).
Copyright © 2002 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- The 9th Asian Pacific Congress of Clinical Biochemistry
- Circulating DNA in Plasma and Serum: Biology, Preanalytical Issues and Diagnostic Applications
- Role of Steroid Hormones and Growth Factors in Breast Cancer
- Gene Polymorphism and Coronary Risk Factors in Indian Population
- Clinical Utility of Free Drug Monitoring
- Telomerase – a Potential Molecular Marker of Lung and Cervical Cancer
- Association of Polymorphisms in the Collagen Region of Human SP-A1 and SP-A2 Genes with Pulmonary Tuberculosis in Indian Population
- Effect of Hemodialysis on the Oxidative Stress and Antioxidants
- Total Iron Binding Capacity and Transferrin Concentration in the Assessment of Iron Status
- Increased Levels of Osteoprotegerin in Hemodialysis Patients
- Levels of Testosterone, Allopregnanolone and Homocysteine in Severe Hypothyroidism
- The Levels of Serum Vitamin C, Malonyldialdehyde and Erythrocyte Reduced Glutathione in Chronic Obstructive Pulmonary Disease and in Healthy Smokers
- Highly Specific, Simple and Rapid Method for the Determination of Malondialdehyde in Blood Using High-Performance Liquid Chromatography
- Precision and Accuracy of a HPLC Method for Measurement of Fecal Porphyrin Concentrations
- Continuous Age-Dependent Reference Ranges for Thyroid Hormones in Neonates, Infants, Children and Adolescents Established Using the ADVIA(r) Centaur(tm) Analyzer
- Diagnostic Value of Various Urine Tests in the Jordanian Population with Urinary Tract Infection
- Performance Evaluation of the Precision™ PCx™ Point-of-Care Blood Glucose Analyzer Using Discriminant Ratio Methodology
- Clinical Evaluation of a New Automated Anti-dsDNA Fluorescent Immunoassay
- Upper Reference Limits for Urinary Catecholamines, Metanephrines and 3-Methoxy-4-Hydroxymandelic Acid in Hypertensive Patients
- Growth Hormone Immunoassays: Proposal to Reduce Interlaboratory Variability
- Poetry, Images and Visions: William Blake
- Meetings and Awards
Articles in the same Issue
- The 9th Asian Pacific Congress of Clinical Biochemistry
- Circulating DNA in Plasma and Serum: Biology, Preanalytical Issues and Diagnostic Applications
- Role of Steroid Hormones and Growth Factors in Breast Cancer
- Gene Polymorphism and Coronary Risk Factors in Indian Population
- Clinical Utility of Free Drug Monitoring
- Telomerase – a Potential Molecular Marker of Lung and Cervical Cancer
- Association of Polymorphisms in the Collagen Region of Human SP-A1 and SP-A2 Genes with Pulmonary Tuberculosis in Indian Population
- Effect of Hemodialysis on the Oxidative Stress and Antioxidants
- Total Iron Binding Capacity and Transferrin Concentration in the Assessment of Iron Status
- Increased Levels of Osteoprotegerin in Hemodialysis Patients
- Levels of Testosterone, Allopregnanolone and Homocysteine in Severe Hypothyroidism
- The Levels of Serum Vitamin C, Malonyldialdehyde and Erythrocyte Reduced Glutathione in Chronic Obstructive Pulmonary Disease and in Healthy Smokers
- Highly Specific, Simple and Rapid Method for the Determination of Malondialdehyde in Blood Using High-Performance Liquid Chromatography
- Precision and Accuracy of a HPLC Method for Measurement of Fecal Porphyrin Concentrations
- Continuous Age-Dependent Reference Ranges for Thyroid Hormones in Neonates, Infants, Children and Adolescents Established Using the ADVIA(r) Centaur(tm) Analyzer
- Diagnostic Value of Various Urine Tests in the Jordanian Population with Urinary Tract Infection
- Performance Evaluation of the Precision™ PCx™ Point-of-Care Blood Glucose Analyzer Using Discriminant Ratio Methodology
- Clinical Evaluation of a New Automated Anti-dsDNA Fluorescent Immunoassay
- Upper Reference Limits for Urinary Catecholamines, Metanephrines and 3-Methoxy-4-Hydroxymandelic Acid in Hypertensive Patients
- Growth Hormone Immunoassays: Proposal to Reduce Interlaboratory Variability
- Poetry, Images and Visions: William Blake
- Meetings and Awards
