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Precision and Accuracy of a HPLC Method for Measurement of Fecal Porphyrin Concentrations

  • Floris M. J. Zuijderhoudt , Johannes S. Kamphuis , Willem E. Kluitenberg and Jenneke Dorresteijn-de Bok
Published/Copyright: June 1, 2005
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Volume 40 Issue 10

Abstract

We studied precision and accuracy of a HPLC method for determination of porphyrins in feces. A commercial standard solution appeared to contain less coproporphyrin (15%) than stated by the manufacturer. The between-batch coefficients of variation were often below 15% and were higher than the within-batch coefficients. The precision of porphyrin measurements was not influenced by the type of porphyria. Recoveries of added coproporphyrin and protoporphyrin were 90% and 108%; coefficients of variation were 6% and 19%, respectively.

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Published Online: 2005-06-01
Published in Print: 2002-10-29

Copyright © 2002 by Walter de Gruyter GmbH & Co. KG

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https://doi.org/10.1515/CCLM.2002.181

Articles in the same Issue

  1. The 9th Asian Pacific Congress of Clinical Biochemistry
  2. Circulating DNA in Plasma and Serum: Biology, Preanalytical Issues and Diagnostic Applications
  3. Role of Steroid Hormones and Growth Factors in Breast Cancer
  4. Gene Polymorphism and Coronary Risk Factors in Indian Population
  5. Clinical Utility of Free Drug Monitoring
  6. Telomerase – a Potential Molecular Marker of Lung and Cervical Cancer
  7. Association of Polymorphisms in the Collagen Region of Human SP-A1 and SP-A2 Genes with Pulmonary Tuberculosis in Indian Population
  8. Effect of Hemodialysis on the Oxidative Stress and Antioxidants
  9. Total Iron Binding Capacity and Transferrin Concentration in the Assessment of Iron Status
  10. Increased Levels of Osteoprotegerin in Hemodialysis Patients
  11. Levels of Testosterone, Allopregnanolone and Homocysteine in Severe Hypothyroidism
  12. The Levels of Serum Vitamin C, Malonyldialdehyde and Erythrocyte Reduced Glutathione in Chronic Obstructive Pulmonary Disease and in Healthy Smokers
  13. Highly Specific, Simple and Rapid Method for the Determination of Malondialdehyde in Blood Using High-Performance Liquid Chromatography
  14. Precision and Accuracy of a HPLC Method for Measurement of Fecal Porphyrin Concentrations
  15. Continuous Age-Dependent Reference Ranges for Thyroid Hormones in Neonates, Infants, Children and Adolescents Established Using the ADVIA(r) Centaur(tm) Analyzer
  16. Diagnostic Value of Various Urine Tests in the Jordanian Population with Urinary Tract Infection
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  18. Clinical Evaluation of a New Automated Anti-dsDNA Fluorescent Immunoassay
  19. Upper Reference Limits for Urinary Catecholamines, Metanephrines and 3-Methoxy-4-Hydroxymandelic Acid in Hypertensive Patients
  20. Growth Hormone Immunoassays: Proposal to Reduce Interlaboratory Variability
  21. Poetry, Images and Visions: William Blake
  22. Meetings and Awards
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