Effect of Hemodialysis on the Oxidative Stress and Antioxidants
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Evrim Dursun
Abstract
Oxidative stress plays a role in many disease states. These diseases have an increased incidence in uremia, and particularly in hemodialysis (HD) patients. This suggests an increased exposure to oxidative stress. An imbalance between oxidants and antioxidants has been suggested in uremic patients on HD. However, the respective influence of uremia and dialysis procedure has not been evaluated. It is postulated that antioxidant capacity in uremic patients is reduced, yet the mechanism remains unclear. We have determined the levels of lipid peroxidation expressed as thiobarbituric acid-reactive substances. We assessed oxidative protein damage by carbonyl content and activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in predialysis uremic patients and in end-stage renal disease (ESRD) patients before and after hemodialysis. Vitamin E and vitamin C levels, reduced glutathione and sulfhydryl content were also studied. We found enhanced oxidative stress in ESRD patients undergoing HD and in predialysis uremic patients. This was mostly due to defective antioxidant enzyme levels. Preventive modalities, including use of biocompatible membranes, ultrapure dialysate, exogenous supplementation of antioxidant vitamins, extracorporeal removal of reactive oxygen species (ROS) and oxidatively modified substances, would appear highly desirable to reduce complications in the long-term dialysis patients.
Copyright © 2002 by Walter de Gruyter GmbH & Co. KG
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- Poetry, Images and Visions: William Blake
- Meetings and Awards
Articles in the same Issue
- The 9th Asian Pacific Congress of Clinical Biochemistry
- Circulating DNA in Plasma and Serum: Biology, Preanalytical Issues and Diagnostic Applications
- Role of Steroid Hormones and Growth Factors in Breast Cancer
- Gene Polymorphism and Coronary Risk Factors in Indian Population
- Clinical Utility of Free Drug Monitoring
- Telomerase – a Potential Molecular Marker of Lung and Cervical Cancer
- Association of Polymorphisms in the Collagen Region of Human SP-A1 and SP-A2 Genes with Pulmonary Tuberculosis in Indian Population
- Effect of Hemodialysis on the Oxidative Stress and Antioxidants
- Total Iron Binding Capacity and Transferrin Concentration in the Assessment of Iron Status
- Increased Levels of Osteoprotegerin in Hemodialysis Patients
- Levels of Testosterone, Allopregnanolone and Homocysteine in Severe Hypothyroidism
- The Levels of Serum Vitamin C, Malonyldialdehyde and Erythrocyte Reduced Glutathione in Chronic Obstructive Pulmonary Disease and in Healthy Smokers
- Highly Specific, Simple and Rapid Method for the Determination of Malondialdehyde in Blood Using High-Performance Liquid Chromatography
- Precision and Accuracy of a HPLC Method for Measurement of Fecal Porphyrin Concentrations
- Continuous Age-Dependent Reference Ranges for Thyroid Hormones in Neonates, Infants, Children and Adolescents Established Using the ADVIA(r) Centaur(tm) Analyzer
- Diagnostic Value of Various Urine Tests in the Jordanian Population with Urinary Tract Infection
- Performance Evaluation of the Precision™ PCx™ Point-of-Care Blood Glucose Analyzer Using Discriminant Ratio Methodology
- Clinical Evaluation of a New Automated Anti-dsDNA Fluorescent Immunoassay
- Upper Reference Limits for Urinary Catecholamines, Metanephrines and 3-Methoxy-4-Hydroxymandelic Acid in Hypertensive Patients
- Growth Hormone Immunoassays: Proposal to Reduce Interlaboratory Variability
- Poetry, Images and Visions: William Blake
- Meetings and Awards
