Topotecan and methotrexate alter expression of the apoptosis-related genes BCL2, FAS and BCL2L12 in leukemic HL-60 cells
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Kostas V. Floros
Abstract
The BCL2 family of genes (B-cell CLL/lymphoma 2; Bcl-2) plays a pivotal role in the highly regulated process of apoptosis. We have recently cloned a newly identified member of this family, BCL2L12, which was found to be differentially expressed in many tumors. It is known that topotecan and methotrexate act through induction of apoptosis in cancer cells. In the present study we investigated the expression profile of the novel apoptotic gene BCL2L12 in relation to other apoptotic genes in the human leukemic cell line HL-60, after treatment with topotecan or methotrexate. The kinetics of apoptosis induction and cell toxicity were investigated by DNA laddering and the MTT method, respectively. Gene expression levels were analyzed by RT-PCR using gene-specific primers. Downregulation of BCL2L12, BCL2 and FAS was observed after treatment of HL-60 cells with topotecan, while treatment with methotrexate led to downregulation of BCL2 and FAS, with no change in BCL2L12 expression. Our results support the significance of mRNA modulations in the expression of apoptosis-related genes during treatment of human leukemic cells with anticancer drugs.
References
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©2006 by Walter de Gruyter Berlin New York
Articles in the same Issue
- Janus-faced role of endothelial NO synthase in vascular disease: uncoupling of oxygen reduction from NO synthesis and its pharmacological reversal
- Leucine aminopeptidases: diversity in structure and function
- Endogenous anti-inflammatory substances, inter-α-inhibitor and bikunin
- Mitochondrial morphology and distribution in mammalian cells
- Heterogeneity in the cysteine protease inhibitor clitocypin gene family
- Mutations in the inter-SH2 domain of the regulatory subunit of phosphoinositide 3-kinase: effects on catalytic subunit binding and holoenzyme function
- Evaluation of Bacillus anthracis thymidine kinase as a potential target for the development of antibacterial nucleoside analogs
- The human malaria parasite Plasmodium falciparum expresses an atypical N-terminally extended pyrophosphokinase with specificity for thiamine
- Fes1p acts as a nucleotide exchange factor for the ribosome-associated molecular chaperone Ssb1p
- Detailed analysis of MIA protein by mutagenesis
- The role of human tissue kallikreins 7 and 8 in intracranial malignancies
- Prognostic significance of the expression of SR-A1, encoding a novel SR-related CTD-associated factor, in breast cancer
- Suppression of TNF-α production by S-adenosylmethionine in human mononuclear leukocytes is not mediated by polyamines
- Topotecan and methotrexate alter expression of the apoptosis-related genes BCL2, FAS and BCL2L12 in leukemic HL-60 cells
- Two secreted cystatins of the soft tick Ornithodoros moubata: differential expression pattern and inhibitory specificity
- Acknowledgment
- Contents Biological Chemistry Volume 387, 2006
- Author Index
- Subject Index
Articles in the same Issue
- Janus-faced role of endothelial NO synthase in vascular disease: uncoupling of oxygen reduction from NO synthesis and its pharmacological reversal
- Leucine aminopeptidases: diversity in structure and function
- Endogenous anti-inflammatory substances, inter-α-inhibitor and bikunin
- Mitochondrial morphology and distribution in mammalian cells
- Heterogeneity in the cysteine protease inhibitor clitocypin gene family
- Mutations in the inter-SH2 domain of the regulatory subunit of phosphoinositide 3-kinase: effects on catalytic subunit binding and holoenzyme function
- Evaluation of Bacillus anthracis thymidine kinase as a potential target for the development of antibacterial nucleoside analogs
- The human malaria parasite Plasmodium falciparum expresses an atypical N-terminally extended pyrophosphokinase with specificity for thiamine
- Fes1p acts as a nucleotide exchange factor for the ribosome-associated molecular chaperone Ssb1p
- Detailed analysis of MIA protein by mutagenesis
- The role of human tissue kallikreins 7 and 8 in intracranial malignancies
- Prognostic significance of the expression of SR-A1, encoding a novel SR-related CTD-associated factor, in breast cancer
- Suppression of TNF-α production by S-adenosylmethionine in human mononuclear leukocytes is not mediated by polyamines
- Topotecan and methotrexate alter expression of the apoptosis-related genes BCL2, FAS and BCL2L12 in leukemic HL-60 cells
- Two secreted cystatins of the soft tick Ornithodoros moubata: differential expression pattern and inhibitory specificity
- Acknowledgment
- Contents Biological Chemistry Volume 387, 2006
- Author Index
- Subject Index