Article
Licensed
Unlicensed
Requires Authentication
Stability of selected serum proteins after long-term storage in the Janus Serum Bank
-
Randi E. Gislefoss
Published/Copyright:
March 17, 2009
Abstract
Background: Human serum from biobanks is frequently used in prospective epidemiological studies. Long-term storage may modify its composition. A better understanding of the stability of the serum components may improve the interpretation of future studies.
Methods: The concentrations of selected proteins; immunoglobulins, carrier proteins and enzymes in samples stored at –25°C for 25 years and 2 years were compared with 1-month-old samples. For each length of storage time, 130 specimens were randomly selected from apparently healthy male blood donors aged 40–49 years. We examined the distribution of values, compared dispersion and localization of central tendency, and established reference intervals for each component.
Results: The study demonstrated non-significant or numerically small group differences in the concentrations of albumin, aspartate amino transferase, cystatin C, immunoglobulin E, immunoglobulin G, and sex hormone binding globulin. Mean values between fresh and 25-year-old samples suggested larger differences during storage for alanine amino transferase (–73.4%), creatinine kinase (–96.1%), insulin C-peptide (–98.7%), ferritin (–18.5%) and transferrin (+8.2%).
Conclusions: The findings showed that long-term storage can introduce a considerable bias for vulnerable components.
Clin Chem Lab Med 2009;47:596–603.
Received: 2008-11-12
Accepted: 2009-1-30
Published Online: 2009-03-17
Published in Print: 2009-05-01
©2009 by Walter de Gruyter Berlin New York
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Review
- Pharmacogenetics of vitamin K antagonists: useful or hype?
- Genetics and Molecular Diagnostics
- Illumina DNA test panel-based genotyping of whole genome amplified-DNA extracted from hair samples: performance and agreement with genotyping results from genomic DNA from buccal cells
- Association of interleukin-18 gene promoter polymorphisms with risk of acute myocardial infarction in northern Chinese Han population
- MYC quantitation in cell-free plasma DNA by real-time PCR for gastric cancer diagnosis
- Predictive value of CA 125 and CA 72-4 in ovarian borderline tumors
- Anti DNA antibodies are not restricted to a specific pattern of fluorescence on HEp2 cells
- General Clinical Chemistry and Laboratory Medicine
- Determination of volatile organic compounds in exhaled breath of patients with lung cancer using solid phase microextraction and gas chromatography mass spectrometry
- Determination of urinary stone composition based on stone morphology: a prospective study of 325 consecutive patients in an emerging country
- Determination of branched chain amino acids, methionine, phenylalanine, tyrosine and α-keto acids in plasma and dried blood samples using HPLC with fluorescence detection
- Hematocytometry analysis as discriminative marker for asthma phenotypes
- A routine method for the quantification of the novel antimycotic drug posaconazole in plasma using liquid chromatography-tandem mass spectrometry
- Reference Values and Biological Variations
- Serum β2-microglobulin measured by immunonephelometry: expression patterns and reference intervals in healthy adults
- Serum enzyme profile characteristics of victims following the Wenchuan earthquake in China
- Stability of selected serum proteins after long-term storage in the Janus Serum Bank
- Spuriously high thyrotropin values due to anti-thyrotropin antibodies in adult patients
- Validation and Outcome Studies
- Workflow restrictions on hematology analyzers XE-2100 and XS-800 when assaying pediatric samples with limited volume
- Letters to the Editor
- Carbamylated hemoglobin remains a critical issue in HbA1c measurements
- Detection of a monoclonal gammopathy by serum index measurement
- Survey on the prevalence of hemolytic specimens in an academic hospital according to collection facility: opportunities for quality improvement
- Quantitative scanning analysis of a cryoglobulin ring detected in sera of patients with hepatitis C using a cooling gel diffusion method
- Significant reduction of erythrocyte glucose-6-phosphate dehydrogenase activity in soccer-players during play. Evidence for catecholamine mediated enzyme inhibition
Keywords for this article
biobank;
biomarker;
blood-based epidemiology;
serum-proteins;
stability;
temperature
Articles in the same Issue
- Review
- Pharmacogenetics of vitamin K antagonists: useful or hype?
- Genetics and Molecular Diagnostics
- Illumina DNA test panel-based genotyping of whole genome amplified-DNA extracted from hair samples: performance and agreement with genotyping results from genomic DNA from buccal cells
- Association of interleukin-18 gene promoter polymorphisms with risk of acute myocardial infarction in northern Chinese Han population
- MYC quantitation in cell-free plasma DNA by real-time PCR for gastric cancer diagnosis
- Predictive value of CA 125 and CA 72-4 in ovarian borderline tumors
- Anti DNA antibodies are not restricted to a specific pattern of fluorescence on HEp2 cells
- General Clinical Chemistry and Laboratory Medicine
- Determination of volatile organic compounds in exhaled breath of patients with lung cancer using solid phase microextraction and gas chromatography mass spectrometry
- Determination of urinary stone composition based on stone morphology: a prospective study of 325 consecutive patients in an emerging country
- Determination of branched chain amino acids, methionine, phenylalanine, tyrosine and α-keto acids in plasma and dried blood samples using HPLC with fluorescence detection
- Hematocytometry analysis as discriminative marker for asthma phenotypes
- A routine method for the quantification of the novel antimycotic drug posaconazole in plasma using liquid chromatography-tandem mass spectrometry
- Reference Values and Biological Variations
- Serum β2-microglobulin measured by immunonephelometry: expression patterns and reference intervals in healthy adults
- Serum enzyme profile characteristics of victims following the Wenchuan earthquake in China
- Stability of selected serum proteins after long-term storage in the Janus Serum Bank
- Spuriously high thyrotropin values due to anti-thyrotropin antibodies in adult patients
- Validation and Outcome Studies
- Workflow restrictions on hematology analyzers XE-2100 and XS-800 when assaying pediatric samples with limited volume
- Letters to the Editor
- Carbamylated hemoglobin remains a critical issue in HbA1c measurements
- Detection of a monoclonal gammopathy by serum index measurement
- Survey on the prevalence of hemolytic specimens in an academic hospital according to collection facility: opportunities for quality improvement
- Quantitative scanning analysis of a cryoglobulin ring detected in sera of patients with hepatitis C using a cooling gel diffusion method
- Significant reduction of erythrocyte glucose-6-phosphate dehydrogenase activity in soccer-players during play. Evidence for catecholamine mediated enzyme inhibition
