Application of umbelliferone molecularly imprinted polymer in analysis of plant samples
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Katarína Hroboňová
,
Andrea Spevak
Abstract
The molecularly imprinted polymers (MIPs) were synthesised and the influence of the type of porogen, the nature of sample solvent, and the binding capacity of material were tested by high-performance liquid chromatography (HPLC). Umbelliferone was used as the template for imprint formation. Methacrylic acid was used as the monomer and acetonitrile, ethanol, and chloroform as porogen. Non-imprinted polymers (NIPs) were prepared by the same procedure. The highest value of the specific binding capacity (269 μg of umbelliferone per 100 mg of polymer) was obtained for polymers prepared in chloroform as porogen and methanol/water (φ r = 1: 1) as the sample solvent. The group-selective MIP was used as sorbent for the SPE pre-treatment of umbelliferone from plant extracts prior to HPLC analysis. Analysis of the spiked samples showed good recoveries (> 77 %). The limit of detection, limit of determination, and repeatability of the method were also calculated.
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© 2013 Institute of Chemistry, Slovak Academy of Sciences
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Artikel in diesem Heft
- Application of umbelliferone molecularly imprinted polymer in analysis of plant samples
- Determination of antioxidant activity using oxidative damage to plasmid DNA — pursuit of solvent optimization
- Nanosized sulfated zirconia as solid acid catalyst for the synthesis of 2-substituted benzimidazoles
- Removal of heavy metal ions from aqueous solutions using low-cost sorbents obtained from ash
- Base-catalysed reduction of pyruvic acid in near-critical water
- Solubility and micronisation of phenacetin in supercritical carbon dioxide
- Synthesis of nanostructured perovskite powders via simple carbonate co-precipitation
- Three-component one-pot reaction for the synthesis of β-amide ketones
- Spectral analysis of naringenin deprotonation in aqueous ethanol solutions
- Provenance study of volcanic glass using 266–1064 nm orthogonal double pulse laser induced breakdown spectroscopy
- A new, fully validated and interpreted quantitative structure-activity relationship model of p-aminosalicylic acid derivatives as neuraminidase inhibitors
- Interaction of oligonucleotides with benzo[c]phenanthridine alkaloid sanguilutine
