Impact of ionic strength on adsorption capacity of chromatographic particles employed in separation of monoclonal antibodies
-
Katarzyna Wrzosek
,
Michal Gramblička
Abstract
The influence of ionic strength on the adsorption capacity of seven commercial adsorbents used in downstream processing of monoclonal antibodies was examined. Affinity (MabSelect, Poros 50A High Capacity, ProSep-vA High Capacity), hydrophobic charge-induction (MEP HyperCel), and cation exchange adsorbents (FractoGel EMD SE Hicap (M), SP Sepharose Fast Flow, Ceramic HyperD F) were used to study the adsorption of polyclonal human immunoglobulin G at optimal pH values. The ionic strength, adjusted by sodium chloride concentrations in the range of 0–225 mM, strongly decreased the adsorption capacity of the cation exchangers. Equilibrium data were described in the form of the dependence of the ratio of protein concentrations in the solid and liquid phases on the total concentration of cation counter ions. They were successfully fitted and interpreted through a stoichiometric ion-exchange model.
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© 2010 Institute of Chemistry, Slovak Academy of Sciences
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- Multi-elemental analysis of marine sediment reference material MESS-3: one-step microwave digestion and determination by high resolution inductively coupled plasma-mass spectrometry (HR-ICP-MS)
- Use of a diazocoupling reaction for sensitive and selective spectrophotometeric determination of furosemide in spiked human urine and pharmaceuticals
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- Liquid chromatographic determination of meloxicam in serum after solid phase extraction
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- Polysaccharide from Anacardium occidentale L. tree gum (Policaju) as a coating for Tommy Atkins mangoes
- In vitro bioactivity and crystallization behavior of bioactive glass in the system SiO2-CaO-Al2O3-P2O5-Na2O-MgO-CaF2
- Synthesis of brushite nanoparticles at different temperatures
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- Vibrational spectroscopic and conformational studies of 1-(4-pyridyl)piperazine
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