Development of a polystyrene latex-based reagent for rheumatoid factor detection
-
Grisel Marrero
Abstract
A rapid, specific and sensible polystyrene latex-based reagent has been developed to detect rheumatoid factor (RF) in human sera. Detection of RF is one of the criteria for rheumatoid arthritis (RA) diagnosis. RF includes immunoglobulins IgG, IgM or IgA targeting human-IgG Fc region, found in sera of 70–90% of patients with RA. Spherical, clean polystyrene particles of 480±80 nm with a Z-potential of -28 mV were synthesized. Purified gamma-globulin human blood fraction, used as IgG source, was adsorbed on particles in glycine-buffered saline with a fivefold excess of the immunoglobulins needed to saturate the calculated total surface area of the particles. The reagent was calibrated against the World Health Organization international serum reference preparation and tested with commercial positive and negative RF controls. Reactivity of the developed diagnostic reagent with human sera, either positive or negative for RF, was evaluated. The sera were tested both intact and following complement inactivation. When complement-inactivated sera were used, our reagent showed 100% coincidence of results with a commercial diagnostic kit of specificity of 80% and sensibility of 93%. The in-house reagent developed in the present work is easy to prepare and at relatively low cost, making it ideal for RF detection in low-income settings.
Acknowledgments
The authors thank Dr. JG Arrieta (Havana, Cuba) for the critical reading of the original manuscript.
References
[1] Gibofsky A. Am. J. Manag. Care. 2014, 20 (7 Suppl), s128–145.Suche in Google Scholar
[2] Aletaha D, Neogi T, Silman AJ, Funovits J, Felson DT, Bingham CO III, Birnbaum NS, Burmester GR, Bykerk VP, Cohen MD, Combe B, Costenbader KH, Dougados M, Emery P, Ferraccioli G, Hazes JMW, Hobbs K, Huizinga TWJ, Kavanaugh A, Kay J, Kvien TK, Laing T, Mease P, Menard HA, Moreland LW, Naden RL, Pincus T, Smolen JS, Stanislawska-Biernat E, Symmons D, Tak PP, Upchurch KS, Vencovsky J, Wolfe F, Hawker G. Arthritis Rheum. 2010, 62, 2569–2581.10.1002/art.27584Suche in Google Scholar PubMed
[3] Landewe Rb. Arthritis Rheum. 2003, 48, 1–5.10.1002/art.10732Suche in Google Scholar PubMed
[4] Westwood OMR, Nelson PN, Hay FC. Rheumatology. 2006, 45, 379–385.10.1093/rheumatology/kei228Suche in Google Scholar PubMed
[5] Ingegnoli F, Castelli R, Gualtierotti R. Dis. Markers. 2013, 35, 727–734.10.1155/2013/726598Suche in Google Scholar PubMed PubMed Central
[6] Artandi SE, Calame KL, Morrison SL, Bonagura VR. Proc. Natl. Acad. Sci. USA. 1992, 89, 94–98.10.1073/pnas.89.1.94Suche in Google Scholar PubMed PubMed Central
[7] Molina-Bolivar JA, Galisteo-Gonzalez F. J. Macromol. Sci. Part C–Polymer. Rev. 2005, 45, 59–98.10.1081/MC-200045819Suche in Google Scholar
[8] Rosado E, Caroll H, Sanchez O, Peniche C. J. Biomater. Sci. Polym. Ed. 2005, 16, 435–448.10.1163/1568562053700165Suche in Google Scholar PubMed
[9] Goodwin JW, Hearn J, Ho CC, Ottewill RH. Br. Polym. J. 1973, 5, 347–362.10.1002/pi.4980050503Suche in Google Scholar
[10] Bangs Laboratories, Inc. Tech. Note 204 Rev. # 003 2013. http://www.bangslabs.com.Suche in Google Scholar
[11] Anderson SG, Bentzon MW, Houba V, Krag P. Bull. Wld. Hlth. Org. 1970, 42, 311–318.10.1016/0042-6822(70)90275-8Suche in Google Scholar
[12] Bangs Laboratories, Inc. Tech. Note 201 Rev. # 004 2013. http://www.bangslabs.com.Suche in Google Scholar
[13] Beckman Coulter, Inc. User’s Manual 2011. http://www.beckmancoulter.com.Suche in Google Scholar
[14] Bas S, Perneger TV, Kunzle E, Vischer TL. Ann. Rheum. Dis. 2002, 61, 505–510.10.1136/ard.61.6.505Suche in Google Scholar PubMed PubMed Central
[15] Silveira-Gomes F, Marques-da-Silva SE. Mem. Inst. Oswaldo Cruz (Rio de Janeiro) 2012, 107, 510–512.10.1590/S0074-02762012000400011Suche in Google Scholar
[16] Murray RK. In Harper’s Illustrated Biochemistry, 26th ed., Foltin, J, Jim, Ransom, Oransky, JM, Eds., McGraw-Hill Companies: New York, USA, 2003, Chapter 50, p 580.Suche in Google Scholar
©2016 by De Gruyter
Artikel in diesem Heft
- Frontmatter
- Review
- The role of poly(acrylic acid) in conventional glass polyalkenoate cements
- Original articles
- Development of a polystyrene latex-based reagent for rheumatoid factor detection
- Biodegradation study of bio-corn flour filled low density polyethylene composites assessed by natural soil
- Efficacy of PU foam materials for scientific investigation in footwear research
- Degradation of PVDF-based composite membrane and its impacts on membrane intrinsic and separation properties
- Fabrication of cellulose fine fiber based membranes embedded with silver nanoparticles via Forcespinning
- Influence of wax content on the electrical, thermal and tribological behaviour of a polyamide 6/graphite composite
- In situ formation of copper nanoparticles in a p(NIPAM-VAA-AAm) terpolymer microgel that retains the swelling behavior of microgels
- Possible application of lead sulfide quantum dot in memory device
- Solution properties of polyaniline/carbon particle composites
- Preparation and characterization of SiO2/fluoroalkyl-trialkoxysilane/2-hydroxyethyl methacrylate/trimethylolpropane triacrylate and SiO2/3-(trimethoxysilyl)propyl methacrylate/ 2-hydroxyethyl methacrylate/trimethylolpropane triacrylate coatings on glass substrates using the sol-gel method
- The performance and morphology of PMMA/SAN/ABS blends
Artikel in diesem Heft
- Frontmatter
- Review
- The role of poly(acrylic acid) in conventional glass polyalkenoate cements
- Original articles
- Development of a polystyrene latex-based reagent for rheumatoid factor detection
- Biodegradation study of bio-corn flour filled low density polyethylene composites assessed by natural soil
- Efficacy of PU foam materials for scientific investigation in footwear research
- Degradation of PVDF-based composite membrane and its impacts on membrane intrinsic and separation properties
- Fabrication of cellulose fine fiber based membranes embedded with silver nanoparticles via Forcespinning
- Influence of wax content on the electrical, thermal and tribological behaviour of a polyamide 6/graphite composite
- In situ formation of copper nanoparticles in a p(NIPAM-VAA-AAm) terpolymer microgel that retains the swelling behavior of microgels
- Possible application of lead sulfide quantum dot in memory device
- Solution properties of polyaniline/carbon particle composites
- Preparation and characterization of SiO2/fluoroalkyl-trialkoxysilane/2-hydroxyethyl methacrylate/trimethylolpropane triacrylate and SiO2/3-(trimethoxysilyl)propyl methacrylate/ 2-hydroxyethyl methacrylate/trimethylolpropane triacrylate coatings on glass substrates using the sol-gel method
- The performance and morphology of PMMA/SAN/ABS blends
