Ameliorative effect of Neera, nonfermented coconut inflorescence sap, on cisplatin-induced renal toxicity by abating oxidative stress
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Prabha Silpa
Abstract
Objectives
Neera, nonfermented coconut inflorescence sap (NFCIS) from unopened spadix of Cocos nucifera L., is a well-known traditional beverage. But, scientific reports on its health benefits are limited. NFCIS is reported to exhibits free radical scavenging activity, and its chemical composition is found promising. In the present study, the effect of NFCIS on alleviating cisplatin-induced nephrotoxicity was analyzed in mice.
Methods
The renal toxicity was induced by cisplatin (16 mg/kg b.wt. ip) in Swiss albino mice. The antioxidant activity of NFCIS was evaluated by nitric oxide radical scavenging assay and phorbol-12-myristate-13-acetate–induced superoxide radical generation in mice peritoneal macrophages. Total polyphenolic content of sap was determined using Folin–Ciocalteu reagent. The phytochemicals present in NFCIS was identified using Fourier transform infrared (FT-IR) spectroscopy.
Results
NFCIS was found to scavenge nitric oxide (NO) radicals (IC50 = 32 ± 2.47 μL/mL) and shown to inhibit superoxide (SO) generation (53.5 ± 2.1%) in macrophages. High polyphenolic content (193 µg gallic acid/mL) was determined in the sap. The FT-IR spectrum of NFCIS revealed the presence of several phytochemicals indicate its pharmaceutical and nutritional value. Cisplatin-induced hike in urea, creatinine and lipid peroxidation was significantly decreased to 65.16, 87.74 and 53.41% by NFCIS, respectively. Hb (42.37%) and total count (72.81%) were also found to be increased. Additionally, the activity of antioxidant enzymes superoxide dismutase, catalase, glutathione peroxidase and reduced glutathione was enhanced to 53.06, 40, 52.22 and 38.49%, respectively.
Conclusions
Results indicate that NFCIS effectively alleviates cisplatin-mediated renal toxicity by its antioxidant activity.
Acknowledgments
The authors acknowledge the Department of Biochemistry, Amala Cancer Research Centre, Thrissur, Kerala for giving the facilities to carry out the research work.
Research funding: None declared.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Ethical approval: All the animal experiments in the present study was carried out with the prior approval from Institutional Animal Ethics Committee (IAEC/ACRC/16-12/17 dated 19/12/2016) were strictly following the guidelines of Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA).
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© 2020 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Review
- Phytochemical profile and pharmacological properties of Trifolium repens
- Original Articles
- Bacopa monnieri alleviates aluminium chloride-induced anxiety by regulating plasma corticosterone level in Wistar rats
- Antidepressant-like effect of Albizia zygia root extract in murine models
- A study of serum cortisol levels in acute head injury patients
- Ferulic acid through mitigation of NMDA receptor pathway exerts anxiolytic-like effect in mouse model of maternal separation stress
- Aberrant pain modulation in trigeminal neuralgia patients
- Phoenix dactylifera Linn fruit based-diets palliate hyperglycemia in alloxan-induced diabetic rats
- Protective effects of Vitex pseudo-negundo leaves on diabetic-induced nephropathy in rats
- Ameliorative effect of Neera, nonfermented coconut inflorescence sap, on cisplatin-induced renal toxicity by abating oxidative stress
- The effect of Rheum ribes L. on oxidative stress in diabetic rats
- Effects of honey supplementation on renal dysfunction and metabolic acidosis in rats with high-fat diet-induced chronic kidney disease
- Co-administration of prazosin and propranolol with glibenclamide improves anti-oxidant defense system in endothelial tissue of streptozotocin-induced diabetic Wistar rats
- Calcium and s100a1 protein balance in the brain–heart axis in diabetic male Wistar rats
- Evaluation of efficacy of Aloe vera (L.) Burm. f. gel solution in methylcellulose-induced ocular hypertension in New Zealand white rabbits
- Haematoprotective and red blood cell membrane stabilizing effects of Justicia carnae leaf extracts in sodium nitrate-treated rats
- Leaf and root bark extracts of Ziziphus abyssinica Hochst ex. A. Rich (Rhamnaceae) ameliorate hepatic, renal and splenic injuries induced by phenylhydrazine in rats
- Antinociceptive, anti-inflammatory and antioxidant effects of Boerhavia coccinea extracts and fractions on acute and persistent inflammatory pain models
- Case Report
- Atorvastatin-linked rhabdomyolysis caused by the simultaneous intake of amoxicillin clavulanic acid
- Miscellaneous
- Acknowledgment
Artikel in diesem Heft
- Review
- Phytochemical profile and pharmacological properties of Trifolium repens
- Original Articles
- Bacopa monnieri alleviates aluminium chloride-induced anxiety by regulating plasma corticosterone level in Wistar rats
- Antidepressant-like effect of Albizia zygia root extract in murine models
- A study of serum cortisol levels in acute head injury patients
- Ferulic acid through mitigation of NMDA receptor pathway exerts anxiolytic-like effect in mouse model of maternal separation stress
- Aberrant pain modulation in trigeminal neuralgia patients
- Phoenix dactylifera Linn fruit based-diets palliate hyperglycemia in alloxan-induced diabetic rats
- Protective effects of Vitex pseudo-negundo leaves on diabetic-induced nephropathy in rats
- Ameliorative effect of Neera, nonfermented coconut inflorescence sap, on cisplatin-induced renal toxicity by abating oxidative stress
- The effect of Rheum ribes L. on oxidative stress in diabetic rats
- Effects of honey supplementation on renal dysfunction and metabolic acidosis in rats with high-fat diet-induced chronic kidney disease
- Co-administration of prazosin and propranolol with glibenclamide improves anti-oxidant defense system in endothelial tissue of streptozotocin-induced diabetic Wistar rats
- Calcium and s100a1 protein balance in the brain–heart axis in diabetic male Wistar rats
- Evaluation of efficacy of Aloe vera (L.) Burm. f. gel solution in methylcellulose-induced ocular hypertension in New Zealand white rabbits
- Haematoprotective and red blood cell membrane stabilizing effects of Justicia carnae leaf extracts in sodium nitrate-treated rats
- Leaf and root bark extracts of Ziziphus abyssinica Hochst ex. A. Rich (Rhamnaceae) ameliorate hepatic, renal and splenic injuries induced by phenylhydrazine in rats
- Antinociceptive, anti-inflammatory and antioxidant effects of Boerhavia coccinea extracts and fractions on acute and persistent inflammatory pain models
- Case Report
- Atorvastatin-linked rhabdomyolysis caused by the simultaneous intake of amoxicillin clavulanic acid
- Miscellaneous
- Acknowledgment
