Antinociceptive, anti-inflammatory and antioxidant effects of Boerhavia coccinea extracts and fractions on acute and persistent inflammatory pain models
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Basile Nganmegne Piegang
Abstract
Background
Boerhavia coccinea (Nyctaginaceae) is an herbaceous plant used for the treatment of pain. The aim of this study was to evaluate the antinociceptive and anti-inflammatory activities of the aqueous (AEBC) and ethanol (EEBC) extracts of Boerhavia coccinea as well as the major fractions (F1, F2 and F3) from EEBC.
Methods
The antinociceptive effect of the extracts and fractions was evaluated using formalin test. AEBC, EEBC and F1 were selected and further evaluated acutely (24 h) and chronically (16 days) in Complete Freund’s Adjuvant (CFA)-induced persistent inflammatory pain for their antihyperalgesic and anti-inflammatory effects. They were administered orally (100 and 200 mg/kg/day) from 48 h following the intraplantar injection of 100 µL of CFA. After the 16 days of chronic treatment, rats’ spinal cord and brain were collected for the evaluation of oxidative stress parameters namely nitric oxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT).
Results
AEBC, EEBC and F1 significantly inhibited the first and second phases of the formalin-induced pain. They significantly reduced the hyperalgesia both in acute and chronic treatments. These extracts showed no acute anti-inflammatory effect. AEBC and EEBC exhibited anti-inflammatory activities after repeated administration. AEBC, EEBC and F1 significantly reduced MDA level and significantly increased SOD and catalase activities, mainly in the spinal cord. AEBC and EEBC also reduced the NO production in the spinal cord.
Conclusions
Boerhavia coccinea extracts and F1 possess potent antinociceptive activity which is not related to their anti-inflammatory properties. Their antioxidant effects may contribute to these activities in chronic treatment.
Funding source: Alexander von Humboldt-Stiftung
Award Identifier / Grant number: 3.4–8151/Nguelefack(19061)
Research funding: The authors gratefully appreciate the support of the Alexander von Humboldt Foundation (Germany) through the equipment subsidy (3.4-8151/Nguelefack (19061)).
Author contributions: TBN and MT designed the work. BNP, FSTN, PPT and FDTB conducted the experiments. TBN, BNP, FSTN and MT analyzed the data and drafted the manuscript. All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: The authors declare that there is no conflict of interest regarding the publication of this article.
Ethical approval: All the procedures were validated by the local ethical committee (025/13/304/FSa) and complied with the laboratory animal Welfare By-Law and the International Association for the Study of Pain (IASP) Guidelines for the Use of Animals in Research.
Data Availability: The data used to support the findings of this study are available from the corresponding author upon request.
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© 2020 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Review
- Phytochemical profile and pharmacological properties of Trifolium repens
- Original Articles
- Bacopa monnieri alleviates aluminium chloride-induced anxiety by regulating plasma corticosterone level in Wistar rats
- Antidepressant-like effect of Albizia zygia root extract in murine models
- A study of serum cortisol levels in acute head injury patients
- Ferulic acid through mitigation of NMDA receptor pathway exerts anxiolytic-like effect in mouse model of maternal separation stress
- Aberrant pain modulation in trigeminal neuralgia patients
- Phoenix dactylifera Linn fruit based-diets palliate hyperglycemia in alloxan-induced diabetic rats
- Protective effects of Vitex pseudo-negundo leaves on diabetic-induced nephropathy in rats
- Ameliorative effect of Neera, nonfermented coconut inflorescence sap, on cisplatin-induced renal toxicity by abating oxidative stress
- The effect of Rheum ribes L. on oxidative stress in diabetic rats
- Effects of honey supplementation on renal dysfunction and metabolic acidosis in rats with high-fat diet-induced chronic kidney disease
- Co-administration of prazosin and propranolol with glibenclamide improves anti-oxidant defense system in endothelial tissue of streptozotocin-induced diabetic Wistar rats
- Calcium and s100a1 protein balance in the brain–heart axis in diabetic male Wistar rats
- Evaluation of efficacy of Aloe vera (L.) Burm. f. gel solution in methylcellulose-induced ocular hypertension in New Zealand white rabbits
- Haematoprotective and red blood cell membrane stabilizing effects of Justicia carnae leaf extracts in sodium nitrate-treated rats
- Leaf and root bark extracts of Ziziphus abyssinica Hochst ex. A. Rich (Rhamnaceae) ameliorate hepatic, renal and splenic injuries induced by phenylhydrazine in rats
- Antinociceptive, anti-inflammatory and antioxidant effects of Boerhavia coccinea extracts and fractions on acute and persistent inflammatory pain models
- Case Report
- Atorvastatin-linked rhabdomyolysis caused by the simultaneous intake of amoxicillin clavulanic acid
- Miscellaneous
- Acknowledgment
Artikel in diesem Heft
- Review
- Phytochemical profile and pharmacological properties of Trifolium repens
- Original Articles
- Bacopa monnieri alleviates aluminium chloride-induced anxiety by regulating plasma corticosterone level in Wistar rats
- Antidepressant-like effect of Albizia zygia root extract in murine models
- A study of serum cortisol levels in acute head injury patients
- Ferulic acid through mitigation of NMDA receptor pathway exerts anxiolytic-like effect in mouse model of maternal separation stress
- Aberrant pain modulation in trigeminal neuralgia patients
- Phoenix dactylifera Linn fruit based-diets palliate hyperglycemia in alloxan-induced diabetic rats
- Protective effects of Vitex pseudo-negundo leaves on diabetic-induced nephropathy in rats
- Ameliorative effect of Neera, nonfermented coconut inflorescence sap, on cisplatin-induced renal toxicity by abating oxidative stress
- The effect of Rheum ribes L. on oxidative stress in diabetic rats
- Effects of honey supplementation on renal dysfunction and metabolic acidosis in rats with high-fat diet-induced chronic kidney disease
- Co-administration of prazosin and propranolol with glibenclamide improves anti-oxidant defense system in endothelial tissue of streptozotocin-induced diabetic Wistar rats
- Calcium and s100a1 protein balance in the brain–heart axis in diabetic male Wistar rats
- Evaluation of efficacy of Aloe vera (L.) Burm. f. gel solution in methylcellulose-induced ocular hypertension in New Zealand white rabbits
- Haematoprotective and red blood cell membrane stabilizing effects of Justicia carnae leaf extracts in sodium nitrate-treated rats
- Leaf and root bark extracts of Ziziphus abyssinica Hochst ex. A. Rich (Rhamnaceae) ameliorate hepatic, renal and splenic injuries induced by phenylhydrazine in rats
- Antinociceptive, anti-inflammatory and antioxidant effects of Boerhavia coccinea extracts and fractions on acute and persistent inflammatory pain models
- Case Report
- Atorvastatin-linked rhabdomyolysis caused by the simultaneous intake of amoxicillin clavulanic acid
- Miscellaneous
- Acknowledgment
