Evaluation of efficacy of Aloe vera (L.) Burm. f. gel solution in methylcellulose-induced ocular hypertension in New Zealand white rabbits
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Jhanvi J. Vaghela
,
Manish J. Barvaliya
Abstract
Objectives
The present study aimed to evaluate the efficacy of Aloe vera in the methylcellulose-induced ocular hypertension model.
Methods
Fifty-six rabbits were randomly divided into seven groups. Intraocular pressure (IOP) was raised by anterior chamber injection of 2% methylcellulose in all the groups except the normal control group. Disease control animals were treated with sterile water and active control with topical 0.5% timolol 12 hourly; 6 and 12% A. vera gel topical solutions 8 hourly were given in group IV and V, respectively; 6 and 12% A. vera gel solutions along with timolol were given in group VI and VII animals, respectively. Mean IOP values and percentage of reduction in IOP were compared at 15 min, 30 min, 1 h, 2 h, 4 h, 8 h, 12 h, 24 h, 36 h, and 48 h.
Results
A. vera gel solutions (6 and 12%) showed a significant reduction in mean IOP from 4 to 2 h time points, respectively, whereas, A. vera gel solutions (6 and 12%) + timolol showed significant less mean IOP from a 30 min time point as compared with disease control. A. vera gel solutions (6 and 12%) showed 8.6 and 10.4% more reduction in IOP, respectively (66.8 ± 4.9% and 68.6 ± 5.4% vs. 58.2 ± 2.3%; p > 0.05), whereas 6 and 12% A. vera gel solutions along with timolol showed 14.5 and 16.2% more reduction in IOP, respectively (72.7 ± 4.7% and 74.4 ± 4.1% vs. 58.2 ± 2.3%; p < 0.05), than disease control group at 48 h.
Conclusions
Six and twelve percent A. vera gel solutions reduced the IOP effectively. Concurrent treatment with 12% A. vera gel and timolol produced the maximum reduction in IOP.
Funding source: Department of Pharmacology, Government Medical College
Acknowledgments
The authors are thankful to Dr. Tejas K. Patel, Associate Professor, Pharmacology, All India Institute of Science, Gorakhpur, Uttar Pradesh, for critical review of the manuscript.
Research funding: Department of Pharmacology, Government Medical College, Bhavnagar-364001, (Gujarat), India.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Ethical approval: All the experiments were performed after a prior approval of the Institutional Animal Ethics Committee (IAEC), Government Medical College, Bhavnagar, Gujarat, India (Approval no.-51/2016, Approval date-25/06/2016). New Zealand white rabbits were procured from the Central Animal House of Government Medical College, Bhavnagar (Registration number is 577/GO/Re/S/02/CPCSEA dated 9th August 2016). All the experiments were performed as per guidelines given by the Committee for the Purpose of Control and Supervision on Experiments on Animals (CPCSEA), Ministry of Forest and Environment, Government of India.
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Artikel in diesem Heft
- Review
- Phytochemical profile and pharmacological properties of Trifolium repens
- Original Articles
- Bacopa monnieri alleviates aluminium chloride-induced anxiety by regulating plasma corticosterone level in Wistar rats
- Antidepressant-like effect of Albizia zygia root extract in murine models
- A study of serum cortisol levels in acute head injury patients
- Ferulic acid through mitigation of NMDA receptor pathway exerts anxiolytic-like effect in mouse model of maternal separation stress
- Aberrant pain modulation in trigeminal neuralgia patients
- Phoenix dactylifera Linn fruit based-diets palliate hyperglycemia in alloxan-induced diabetic rats
- Protective effects of Vitex pseudo-negundo leaves on diabetic-induced nephropathy in rats
- Ameliorative effect of Neera, nonfermented coconut inflorescence sap, on cisplatin-induced renal toxicity by abating oxidative stress
- The effect of Rheum ribes L. on oxidative stress in diabetic rats
- Effects of honey supplementation on renal dysfunction and metabolic acidosis in rats with high-fat diet-induced chronic kidney disease
- Co-administration of prazosin and propranolol with glibenclamide improves anti-oxidant defense system in endothelial tissue of streptozotocin-induced diabetic Wistar rats
- Calcium and s100a1 protein balance in the brain–heart axis in diabetic male Wistar rats
- Evaluation of efficacy of Aloe vera (L.) Burm. f. gel solution in methylcellulose-induced ocular hypertension in New Zealand white rabbits
- Haematoprotective and red blood cell membrane stabilizing effects of Justicia carnae leaf extracts in sodium nitrate-treated rats
- Leaf and root bark extracts of Ziziphus abyssinica Hochst ex. A. Rich (Rhamnaceae) ameliorate hepatic, renal and splenic injuries induced by phenylhydrazine in rats
- Antinociceptive, anti-inflammatory and antioxidant effects of Boerhavia coccinea extracts and fractions on acute and persistent inflammatory pain models
- Case Report
- Atorvastatin-linked rhabdomyolysis caused by the simultaneous intake of amoxicillin clavulanic acid
- Miscellaneous
- Acknowledgment
