Erythritol biosynthesis from glycerol by Yarrowia lipolytica yeast: effect of osmotic pressure
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Ludwika Tomaszewska-Hetman
und
Anita Rywińska
Abstract
The aim of this study was to examine the impact of osmotic pressure, regulated by an addition of different NaCl concentrations, on the production parameters and activity of the enzymes involved in the biosynthesis of erythritol from glycerol by Yarrowia lipolytica yeast. In the bioreactor batchcultures, strain A-3 was able to produce from 25.3 g dm-3 to 84.7 g dm-3 of erythritol from 150 g dm-3 of glycerol depending on the initial osmotic pressure. At the osmolality of 4.2 mol kg-3 or higher, a long lag-phase was observed. An enhancement of the production parameters was observed in a culture with the osmotic pressure maintained at an equal level by a step-wise addition of NaCl. The two-hour exposure of strain A-3 cells to 75 g dm-3 of NaCl resulted in decreased activity of glycerol kinase and glycerol-3-phosphate dehydrogenase by about 78 % and 25 %, respectively. The activity of transketolase and erythrose reductase remained unchanged after the salt addition. It was demonstrated that assimilation of glycerol was effective at lower osmotic pressures and that transketolase and erythrose reductase played a significant role in the erythritol formation in Y. lipolytica.
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Institute of Chemistry, Slovak Academy of Sciences
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Artikel in diesem Heft
- Synthesis and properties of new N,N′-phenyltetrazole podand
- Molecular diagnosis of Pompe disease using MALDI TOF/TOF and 1H NMR
- Erythritol biosynthesis from glycerol by Yarrowia lipolytica yeast: effect of osmotic pressure
- Cloning and expression of two genes coding endo-β-1,4-glucanases from Trichoderma asperellum PQ34 in Pichia pastoris
- Adsorption desulphurisation of dimethyl sulphide using nickel-based Y zeolites pretreated by hydrogen reduction
- Equilibrium and kinetics of wetting hydrophobic microporous membrane in sodium dodecyl benzene sulphonate and diethanolamine aqueous solutions
- Separation of urea adducts in the analysis of complex mineral fertilisers
- Cheese whey tangential filtration using tubular mineral membranes
- Characterization of the quality of novel rye-buckwheat ginger cakes by chemical markers and antioxidant capacity
- A new high-temperature inorganic–organic proton conductor: lanthanum sulfophenyl phosphate
- Membranes with a plasma deposited titanium isopropoxide layer
- Effect of fuel content on formation of zinc aluminate nano and micro-particles synthesised by high rate sol–gel autoignition of glycine-nitrates
- Poly(butyl cyanoacrylate) nanoparticles stabilised with poloxamer 188: particle size control and cytotoxic effects in cervical carcinoma (HeLa) cells
- Solubility enhancement of phenanthrene using novel chelating surfactant
- Physicochemical and excess properties of binary mixtures of (1-alkyl-3-methylimidazoliumchloride/bromide + ethylene glycol) at T = (288.15 to 333.15) K
