Diagnosis and evaluation of severity of sepsis via the use of biomarkers and profiles of 13 cytokines: a multiplex analysis
-
Dong Wook Jekarl
,
Kyungja Han
Abstract
Background: The object of this study was to evaluate biomarkers for diagnosis of sepsis, hematologic parameters, and cytokine profiles for use in the diagnosis and evaluation of severity of sepsis.
Methods: We enrolled 127 consecutive patients with systemic inflammatory response syndrome (SIRS), 97 of whom were diagnosed with sepsis. The following biomarkers were evaluated: procalcitonin (PCT); C-reactive protein (CRP); erythrocyte sedimentation rate (ESR); white blood cell count, immature granulocyte (IG) count; and multiplex cytokines, including interleukin (IL)1-β (IL1β), IL2, IL4, IL5, IL6, IL9, IL10, IL12p70, IL13, IL17, IL22, tumor necrosis factor-α (TNFα), and interferon-γ (IFNγ). A cytokine bead immunoassay was used to perform simultaneous measurements.
Results: The disease involving urinary and respiratory tract constituted 57.5% of all patients. The severity of infection was classified as follows: SIRS patients, n=30; sepsis patients, n=81; and septic shock/severe sepsis patients, n=16. PCT, IL6, and CRP had high area under receiver operation characteristic curve (AUCs) and accuracy, which is as follows: PCT: 0.841, 80.5%; IL6: 0.811, 77.1%; CRP: 0.784, 73.8%, respectively. Severity of sepsis could be discriminated by PCT, IL6, and IL5. Unlike other cytokines, IFNγ had an inverse relation with severity of sepsis. The relationship between cytokine profiles and clinical diagnosis of sepsis was unclear.
Conclusions: PCT, IL6, and CRP values could assist diagnosis, and PCT, IL6, and IL5 had discriminative properties for determination of severity of sepsis. IFNγ revealed a distinct inverse relationship with severity of sepsis. As there was no relationship between cytokine profiles and sepsis, further studies are required to develop clinical applications.
Acknowledgments
We thank Hyunyu Choi, Bumse Son, and Hyuk Kwon for excellent technical assistance. All the reagents are provided by Dong Kang Medipia, Co., Ltd., which was arranged by Dong Hwan Kim.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Financial support: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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Supplemental Material
The online version of this article (DOI: 10.1515/cclm-2014-0607) offers supplementary material, available to authorized users.
©2015 by De Gruyter
Artikel in diesem Heft
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- “Personalized” sepsis care with the help of specific biomarker levels on admission and during follow up: are we there yet?
- Procalcitonin-guided antibiotic therapy: a potentially effective and efficient strategy
- Review
- The prognostic blood biomarker proadrenomedullin for outcome prediction in patients with chronic obstructive pulmonary disease (COPD): a qualitative clinical review
- General Clinical Chemistry and Laboratory Medicine
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- Diagnosis and evaluation of severity of sepsis via the use of biomarkers and profiles of 13 cytokines: a multiplex analysis
- Economic evaluation of procalcitonin-guided antibiotic therapy in acute respiratory infections: a US health system perspective
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- Letters to the Editors
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- Expression of CD64 on neutrophils (CD64 index): diagnostic accuracy of CD64 index to predict sepsis in critically ill patients
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Artikel in diesem Heft
- Frontmatter
- Editorials
- “Personalized” sepsis care with the help of specific biomarker levels on admission and during follow up: are we there yet?
- Procalcitonin-guided antibiotic therapy: a potentially effective and efficient strategy
- Review
- The prognostic blood biomarker proadrenomedullin for outcome prediction in patients with chronic obstructive pulmonary disease (COPD): a qualitative clinical review
- General Clinical Chemistry and Laboratory Medicine
- Diagnostic testing for a high-grade inflammation: parameter dynamics and novel markers
- Serial changes in serum procalcitonin, interleukin 6, and C-reactive protein levels according to non-specific surgical stimulation
- Utility of procalcitonin, C-reactive protein and white blood cells alone and in combination for the prediction of clinical outcomes in community-acquired pneumonia
- Presepsin as a potential marker for bacterial infection relapse in critical care patients. A preliminary study
- Diagnosis and evaluation of severity of sepsis via the use of biomarkers and profiles of 13 cytokines: a multiplex analysis
- Economic evaluation of procalcitonin-guided antibiotic therapy in acute respiratory infections: a US health system perspective
- Analytical evaluation of Diazyme procalcitonin (PCT) latex-enhanced immunoturbidimetric assay on Beckman Coulter AU5800
- Calprotectin and lactoferrin in the cerebrospinal fluid; biomarkers utilisable for differential diagnostics of bacterial and aseptic meningitis?
- Cancer Diagnostics
- Increased level of circulating U2 small nuclear RNA fragments indicates metastasis in melanoma patients
- Cardiovascular Diseases
- Comparison between admission natriuretic peptides, NGAL and sST2 testing for the prediction of worsening renal function in patients with acutely decompensated heart failure
- Diabetes
- Weight loss reduces serum monocyte chemoattractant protein-1 concentrations in association with improvements in renal injury in obese men with metabolic syndrome
- Letters to the Editors
- Ranking prestige of medical and laboratory technology journals
- Expression of CD64 on neutrophils (CD64 index): diagnostic accuracy of CD64 index to predict sepsis in critically ill patients
- Measurement of apolipoprotein M in sepsis-related deaths
- The stability of select biomarkers in frozen plasma over time: an evaluation of a low-volume sample analyzer
- Is the new Beckman AccuTnI+3 assay capable of producing false-positive troponin I results?
- Spectrum of red cell abnormalities in undiagnosed hemolytic anemias and methemoglobinemias: a single center experience
- Correlation of methadone concentrations in plasma and saliva collected with and without stimulation in pain management patients
- Higher alkaline phosphatase was associated with the short-term adverse outcomes of peritoneal dialysis-related peritonitis
- The use of a rapid fluorogenic neuraminidase assay to differentiate acute Streptococcus pneumoniae-associated hemolytic uremic syndrome (HUS) from other forms of HUS
- Congress Abstracts
- 3rd EFLM-BD European Conference on Preanalytical Phase
