Increased level of circulating U2 small nuclear RNA fragments indicates metastasis in melanoma patients
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Jan Dominik Kuhlmann
,
Pauline Wimberger
Abstract
Background: Melanoma is the most aggressive skin cancer and, despite recent advances in therapy, about 20% of the patients die of their disease. Early relapse detection and monitoring of therapy response are crucial for efficient treatment of advanced melanoma. Thus, there is a need for blood-based biomarkers in melanoma management. Serum-derived U2 small nuclear RNA fragments (RNU2-1f) were previously shown to be blood-based biomarkers for gastrointestinal and gynecologic malignancies. Here we examined whether RNU2-1f may also serve as diagnostic biomarker in advanced melanoma.
Methods: Circulating RNU2-1f levels were quantified by comparative reverse transcription PCR in a training cohort of patients with metastatic melanoma (n=33, thereof regionally metastasized to skin and lymph nodes, n=23, and distantly metastasized, n=10) vs. patients with benign naevi (n=16) vs. healthy controls (n=39). Results were validated in an independent patient cohort with distant metastasis (n=16) vs. controls (n=18).
Results: Circulating RNU2-1f levels in the training cohort were significantly increased in serum of regionally and distantly metastatic patients, compared with patients with benign naevi or healthy controls (p<0.0001) and allowed accurate detection of regional (AUC 0.80) as well as distant (AUC 0.84) metastasis. In the validation cohort, increased RNU2-1f levels were confirmed and enabled highly specific detection of distant metastasis (sensitivity 81%, specificity 100%, AUC 0.94).
Conclusions: This is the first report to suggest a blood-based snRNA serving as a diagnostic biomarker for melanoma metastasis. Our data provide a rationale for further defining clinical utility of circulating RNU2-1f in metastasis detection in the management of melanoma patients at risk of relapse and/or with advanced disease.
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©2015 by De Gruyter
Artikel in diesem Heft
- Frontmatter
- Editorials
- “Personalized” sepsis care with the help of specific biomarker levels on admission and during follow up: are we there yet?
- Procalcitonin-guided antibiotic therapy: a potentially effective and efficient strategy
- Review
- The prognostic blood biomarker proadrenomedullin for outcome prediction in patients with chronic obstructive pulmonary disease (COPD): a qualitative clinical review
- General Clinical Chemistry and Laboratory Medicine
- Diagnostic testing for a high-grade inflammation: parameter dynamics and novel markers
- Serial changes in serum procalcitonin, interleukin 6, and C-reactive protein levels according to non-specific surgical stimulation
- Utility of procalcitonin, C-reactive protein and white blood cells alone and in combination for the prediction of clinical outcomes in community-acquired pneumonia
- Presepsin as a potential marker for bacterial infection relapse in critical care patients. A preliminary study
- Diagnosis and evaluation of severity of sepsis via the use of biomarkers and profiles of 13 cytokines: a multiplex analysis
- Economic evaluation of procalcitonin-guided antibiotic therapy in acute respiratory infections: a US health system perspective
- Analytical evaluation of Diazyme procalcitonin (PCT) latex-enhanced immunoturbidimetric assay on Beckman Coulter AU5800
- Calprotectin and lactoferrin in the cerebrospinal fluid; biomarkers utilisable for differential diagnostics of bacterial and aseptic meningitis?
- Cancer Diagnostics
- Increased level of circulating U2 small nuclear RNA fragments indicates metastasis in melanoma patients
- Cardiovascular Diseases
- Comparison between admission natriuretic peptides, NGAL and sST2 testing for the prediction of worsening renal function in patients with acutely decompensated heart failure
- Diabetes
- Weight loss reduces serum monocyte chemoattractant protein-1 concentrations in association with improvements in renal injury in obese men with metabolic syndrome
- Letters to the Editors
- Ranking prestige of medical and laboratory technology journals
- Expression of CD64 on neutrophils (CD64 index): diagnostic accuracy of CD64 index to predict sepsis in critically ill patients
- Measurement of apolipoprotein M in sepsis-related deaths
- The stability of select biomarkers in frozen plasma over time: an evaluation of a low-volume sample analyzer
- Is the new Beckman AccuTnI+3 assay capable of producing false-positive troponin I results?
- Spectrum of red cell abnormalities in undiagnosed hemolytic anemias and methemoglobinemias: a single center experience
- Correlation of methadone concentrations in plasma and saliva collected with and without stimulation in pain management patients
- Higher alkaline phosphatase was associated with the short-term adverse outcomes of peritoneal dialysis-related peritonitis
- The use of a rapid fluorogenic neuraminidase assay to differentiate acute Streptococcus pneumoniae-associated hemolytic uremic syndrome (HUS) from other forms of HUS
- Congress Abstracts
- 3rd EFLM-BD European Conference on Preanalytical Phase
Artikel in diesem Heft
- Frontmatter
- Editorials
- “Personalized” sepsis care with the help of specific biomarker levels on admission and during follow up: are we there yet?
- Procalcitonin-guided antibiotic therapy: a potentially effective and efficient strategy
- Review
- The prognostic blood biomarker proadrenomedullin for outcome prediction in patients with chronic obstructive pulmonary disease (COPD): a qualitative clinical review
- General Clinical Chemistry and Laboratory Medicine
- Diagnostic testing for a high-grade inflammation: parameter dynamics and novel markers
- Serial changes in serum procalcitonin, interleukin 6, and C-reactive protein levels according to non-specific surgical stimulation
- Utility of procalcitonin, C-reactive protein and white blood cells alone and in combination for the prediction of clinical outcomes in community-acquired pneumonia
- Presepsin as a potential marker for bacterial infection relapse in critical care patients. A preliminary study
- Diagnosis and evaluation of severity of sepsis via the use of biomarkers and profiles of 13 cytokines: a multiplex analysis
- Economic evaluation of procalcitonin-guided antibiotic therapy in acute respiratory infections: a US health system perspective
- Analytical evaluation of Diazyme procalcitonin (PCT) latex-enhanced immunoturbidimetric assay on Beckman Coulter AU5800
- Calprotectin and lactoferrin in the cerebrospinal fluid; biomarkers utilisable for differential diagnostics of bacterial and aseptic meningitis?
- Cancer Diagnostics
- Increased level of circulating U2 small nuclear RNA fragments indicates metastasis in melanoma patients
- Cardiovascular Diseases
- Comparison between admission natriuretic peptides, NGAL and sST2 testing for the prediction of worsening renal function in patients with acutely decompensated heart failure
- Diabetes
- Weight loss reduces serum monocyte chemoattractant protein-1 concentrations in association with improvements in renal injury in obese men with metabolic syndrome
- Letters to the Editors
- Ranking prestige of medical and laboratory technology journals
- Expression of CD64 on neutrophils (CD64 index): diagnostic accuracy of CD64 index to predict sepsis in critically ill patients
- Measurement of apolipoprotein M in sepsis-related deaths
- The stability of select biomarkers in frozen plasma over time: an evaluation of a low-volume sample analyzer
- Is the new Beckman AccuTnI+3 assay capable of producing false-positive troponin I results?
- Spectrum of red cell abnormalities in undiagnosed hemolytic anemias and methemoglobinemias: a single center experience
- Correlation of methadone concentrations in plasma and saliva collected with and without stimulation in pain management patients
- Higher alkaline phosphatase was associated with the short-term adverse outcomes of peritoneal dialysis-related peritonitis
- The use of a rapid fluorogenic neuraminidase assay to differentiate acute Streptococcus pneumoniae-associated hemolytic uremic syndrome (HUS) from other forms of HUS
- Congress Abstracts
- 3rd EFLM-BD European Conference on Preanalytical Phase
