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Diagnostic testing for a high-grade inflammation: parameter dynamics and novel markers

  • Christina Hoffmann ORCID logo EMAIL logo , Peter Hoffmann and Mathias Zimmermann
Published/Copyright: August 7, 2014
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 53 Issue 4

Abstract

Background: The timely detection of severe inflammatory conditions is of great importance for early therapy initiation and the patient’s prognosis. The complex humoral and cellular processes involved in inflammation offer many opportunities for diagnostic testing, which are still unused in clinical practice. We investigated the dynamics of four established and two novel potential markers during the onset and resolution of a high-grade inflammation.

Methods: We retrospectively analyzed C-reactive protein and procalcitonin concentrations, leukocyte and thrombocyte counts, granularity index, and δ-hemoglobin measured in peripheral blood samples of patients undergoing inflammation diagnostics between September 2010 and November 2010. Data from a consecutive sample of 53,968 patients were available.

Results: Trajectories for the parameters’ dynamics during the onset and resolution of a high-grade inflammation were calculated with a locally weighted scatter plot smoothing method. The leukocyte count trajectories did not exceed the reference range.

Conclusions: We were able to elucidate the parameter dynamics with time coordinates rounded to the nearest hour and a follow-up of 168 h.

Keywords: acute phase reaction; clinical laboratory techniques; critical care
Corresponding author: Christina Hoffmann, Charité – Universitätsmedizin Berlin, Institut für Laboratoriumsmedizin, Klinische Chemie und Pathobiochemie, Augustenburger Platz 1, 13353 Berlin, Deutschland, Phone: +49 30 450569034, Fax: +49 30 450569900, E-mail: .
aChristina Hoffmann and Peter Hoffmann contributed equally to this work.

Acknowledgments

We thank Jose B. Gonzalez for his input to study conception. We are further grateful to Henrik Schmidt for his assistance with data processing.

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Financial support: None declared.

Employment or leadership: None declared.

Honorarium: M.Z. received a travel grant from Sysmex Deutschland GmbH.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2014-5-5
Accepted: 2014-7-4
Published Online: 2014-8-7
Published in Print: 2015-3-1

©2015 by De Gruyter

Articles in the same Issue

  1. Frontmatter
  2. Editorials
  3. “Personalized” sepsis care with the help of specific biomarker levels on admission and during follow up: are we there yet?
  4. Procalcitonin-guided antibiotic therapy: a potentially effective and efficient strategy
  5. Review
  6. The prognostic blood biomarker proadrenomedullin for outcome prediction in patients with chronic obstructive pulmonary disease (COPD): a qualitative clinical review
  7. General Clinical Chemistry and Laboratory Medicine
  8. Diagnostic testing for a high-grade inflammation: parameter dynamics and novel markers
  9. Serial changes in serum procalcitonin, interleukin 6, and C-reactive protein levels according to non-specific surgical stimulation
  10. Utility of procalcitonin, C-reactive protein and white blood cells alone and in combination for the prediction of clinical outcomes in community-acquired pneumonia
  11. Presepsin as a potential marker for bacterial infection relapse in critical care patients. A preliminary study
  12. Diagnosis and evaluation of severity of sepsis via the use of biomarkers and profiles of 13 cytokines: a multiplex analysis
  13. Economic evaluation of procalcitonin-guided antibiotic therapy in acute respiratory infections: a US health system perspective
  14. Analytical evaluation of Diazyme procalcitonin (PCT) latex-enhanced immunoturbidimetric assay on Beckman Coulter AU5800
  15. Calprotectin and lactoferrin in the cerebrospinal fluid; biomarkers utilisable for differential diagnostics of bacterial and aseptic meningitis?
  16. Cancer Diagnostics
  17. Increased level of circulating U2 small nuclear RNA fragments indicates metastasis in melanoma patients
  18. Cardiovascular Diseases
  19. Comparison between admission natriuretic peptides, NGAL and sST2 testing for the prediction of worsening renal function in patients with acutely decompensated heart failure
  20. Diabetes
  21. Weight loss reduces serum monocyte chemoattractant protein-1 concentrations in association with improvements in renal injury in obese men with metabolic syndrome
  22. Letters to the Editors
  23. Ranking prestige of medical and laboratory technology journals
  24. Expression of CD64 on neutrophils (CD64 index): diagnostic accuracy of CD64 index to predict sepsis in critically ill patients
  25. Measurement of apolipoprotein M in sepsis-related deaths
  26. The stability of select biomarkers in frozen plasma over time: an evaluation of a low-volume sample analyzer
  27. Is the new Beckman AccuTnI+3 assay capable of producing false-positive troponin I results?
  28. Spectrum of red cell abnormalities in undiagnosed hemolytic anemias and methemoglobinemias: a single center experience
  29. Correlation of methadone concentrations in plasma and saliva collected with and without stimulation in pain management patients
  30. Higher alkaline phosphatase was associated with the short-term adverse outcomes of peritoneal dialysis-related peritonitis
  31. The use of a rapid fluorogenic neuraminidase assay to differentiate acute Streptococcus pneumoniae-associated hemolytic uremic syndrome (HUS) from other forms of HUS
  32. Congress Abstracts
  33. 3rd EFLM-BD European Conference on Preanalytical Phase
https://doi.org/10.1515/cclm-2014-0482
Keywords for this article
acute phase reaction; clinical laboratory techniques; critical care

Articles in the same Issue

  1. Frontmatter
  2. Editorials
  3. “Personalized” sepsis care with the help of specific biomarker levels on admission and during follow up: are we there yet?
  4. Procalcitonin-guided antibiotic therapy: a potentially effective and efficient strategy
  5. Review
  6. The prognostic blood biomarker proadrenomedullin for outcome prediction in patients with chronic obstructive pulmonary disease (COPD): a qualitative clinical review
  7. General Clinical Chemistry and Laboratory Medicine
  8. Diagnostic testing for a high-grade inflammation: parameter dynamics and novel markers
  9. Serial changes in serum procalcitonin, interleukin 6, and C-reactive protein levels according to non-specific surgical stimulation
  10. Utility of procalcitonin, C-reactive protein and white blood cells alone and in combination for the prediction of clinical outcomes in community-acquired pneumonia
  11. Presepsin as a potential marker for bacterial infection relapse in critical care patients. A preliminary study
  12. Diagnosis and evaluation of severity of sepsis via the use of biomarkers and profiles of 13 cytokines: a multiplex analysis
  13. Economic evaluation of procalcitonin-guided antibiotic therapy in acute respiratory infections: a US health system perspective
  14. Analytical evaluation of Diazyme procalcitonin (PCT) latex-enhanced immunoturbidimetric assay on Beckman Coulter AU5800
  15. Calprotectin and lactoferrin in the cerebrospinal fluid; biomarkers utilisable for differential diagnostics of bacterial and aseptic meningitis?
  16. Cancer Diagnostics
  17. Increased level of circulating U2 small nuclear RNA fragments indicates metastasis in melanoma patients
  18. Cardiovascular Diseases
  19. Comparison between admission natriuretic peptides, NGAL and sST2 testing for the prediction of worsening renal function in patients with acutely decompensated heart failure
  20. Diabetes
  21. Weight loss reduces serum monocyte chemoattractant protein-1 concentrations in association with improvements in renal injury in obese men with metabolic syndrome
  22. Letters to the Editors
  23. Ranking prestige of medical and laboratory technology journals
  24. Expression of CD64 on neutrophils (CD64 index): diagnostic accuracy of CD64 index to predict sepsis in critically ill patients
  25. Measurement of apolipoprotein M in sepsis-related deaths
  26. The stability of select biomarkers in frozen plasma over time: an evaluation of a low-volume sample analyzer
  27. Is the new Beckman AccuTnI+3 assay capable of producing false-positive troponin I results?
  28. Spectrum of red cell abnormalities in undiagnosed hemolytic anemias and methemoglobinemias: a single center experience
  29. Correlation of methadone concentrations in plasma and saliva collected with and without stimulation in pain management patients
  30. Higher alkaline phosphatase was associated with the short-term adverse outcomes of peritoneal dialysis-related peritonitis
  31. The use of a rapid fluorogenic neuraminidase assay to differentiate acute Streptococcus pneumoniae-associated hemolytic uremic syndrome (HUS) from other forms of HUS
  32. Congress Abstracts
  33. 3rd EFLM-BD European Conference on Preanalytical Phase
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