Calprotectin and lactoferrin in the cerebrospinal fluid; biomarkers utilisable for differential diagnostics of bacterial and aseptic meningitis?
-
Milan Dastych
,
Jana Gottwaldová
Abstract
Background: The aim of our work was to assess the diagnostic contribution of calprotectin and lactoferrin determinations in the cerebrospinal fluid when distinguishing between bacterial and aseptic meningitides.
Methods: In 23 patients with bacterial meningitis (BM) and in 50 patients with aseptic meningitis (AM), we determined the concentrations of calprotectin, lactoferrin and the conventional biomarkers like glucose, total protein, lactate and polynuclear count in the cerebrospinal fluid (CSF). The discriminative power of the various parameters studied was determined by means of receiver operating characteristic (ROC) curves: the area under the curve (AUC), sensitivity, specificity, the positive likelihood ratio (+LR), and the negative likelihood ratio (–LR).
Results: The diagnostic efficiency of calprotectin, lactoferrin, lactate, and polynuclear count when distinguishing between bacterial and aseptic meningitides, expressed by ROC curve parameters, was as follows: AUC (0.736, 0.946, 0.932, 0.932), sensitivity (86.2, 96.6, 90.0, 89.7), specificity (58.5, 92.4, 87.0, 90.6), +LR (2.08, 12.8, 6.9, 9.50), –LR (0.24, 0.04, 0.11, 0.11), respectively. The optimal cut point for calprotectin and lactoferrin was 191 ng/mL and 17.8 ng/mL, respectively.
Conclusions: Our findings show, that the determination of lactoferrin in the CSF was diagnostically the most efficient marker in distinguishing between bacterial and viral meningitides. Calprotectin was far less efficient diagnostic marker. The polynuclear count and lactate concentration showed a very good diagnostic efficiency as well. The determination of protein and glucose was diagnostically less beneficial.
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©2015 by De Gruyter
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Articles in the same Issue
- Frontmatter
- Editorials
- “Personalized” sepsis care with the help of specific biomarker levels on admission and during follow up: are we there yet?
- Procalcitonin-guided antibiotic therapy: a potentially effective and efficient strategy
- Review
- The prognostic blood biomarker proadrenomedullin for outcome prediction in patients with chronic obstructive pulmonary disease (COPD): a qualitative clinical review
- General Clinical Chemistry and Laboratory Medicine
- Diagnostic testing for a high-grade inflammation: parameter dynamics and novel markers
- Serial changes in serum procalcitonin, interleukin 6, and C-reactive protein levels according to non-specific surgical stimulation
- Utility of procalcitonin, C-reactive protein and white blood cells alone and in combination for the prediction of clinical outcomes in community-acquired pneumonia
- Presepsin as a potential marker for bacterial infection relapse in critical care patients. A preliminary study
- Diagnosis and evaluation of severity of sepsis via the use of biomarkers and profiles of 13 cytokines: a multiplex analysis
- Economic evaluation of procalcitonin-guided antibiotic therapy in acute respiratory infections: a US health system perspective
- Analytical evaluation of Diazyme procalcitonin (PCT) latex-enhanced immunoturbidimetric assay on Beckman Coulter AU5800
- Calprotectin and lactoferrin in the cerebrospinal fluid; biomarkers utilisable for differential diagnostics of bacterial and aseptic meningitis?
- Cancer Diagnostics
- Increased level of circulating U2 small nuclear RNA fragments indicates metastasis in melanoma patients
- Cardiovascular Diseases
- Comparison between admission natriuretic peptides, NGAL and sST2 testing for the prediction of worsening renal function in patients with acutely decompensated heart failure
- Diabetes
- Weight loss reduces serum monocyte chemoattractant protein-1 concentrations in association with improvements in renal injury in obese men with metabolic syndrome
- Letters to the Editors
- Ranking prestige of medical and laboratory technology journals
- Expression of CD64 on neutrophils (CD64 index): diagnostic accuracy of CD64 index to predict sepsis in critically ill patients
- Measurement of apolipoprotein M in sepsis-related deaths
- The stability of select biomarkers in frozen plasma over time: an evaluation of a low-volume sample analyzer
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- Spectrum of red cell abnormalities in undiagnosed hemolytic anemias and methemoglobinemias: a single center experience
- Correlation of methadone concentrations in plasma and saliva collected with and without stimulation in pain management patients
- Higher alkaline phosphatase was associated with the short-term adverse outcomes of peritoneal dialysis-related peritonitis
- The use of a rapid fluorogenic neuraminidase assay to differentiate acute Streptococcus pneumoniae-associated hemolytic uremic syndrome (HUS) from other forms of HUS
- Congress Abstracts
- 3rd EFLM-BD European Conference on Preanalytical Phase
