Production of endoglucanase from Trichoderma reesei RUT C30 and its application in deinking of printed office waste paper

Ayman Salih Omer Idris; Ashok Pandey; Rajeev Kumar Sukumaran

doi:10.1515/biolog-2016-0046

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Production of endoglucanase from Trichoderma reesei RUT C30 and its application in deinking of printed office waste paper

Ayman Salih Omer Idris , Ashok Pandey und Rajeev Kumar Sukumaran

Veröffentlicht/Copyright: 20. April 2016

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Aus der Zeitschrift Biologia Band 71 Heft 3

Abstract

Media components were optimized using two-step statistical design of experiments for enhancing endoglucanase/ carboxymethyl cellulase (CMCase) production by Trichoderma reesei RUT C30. A Placket-Burman design identified cellulose concentration and pH as the most significant variables, which influenced the CMCase activity. Central composite design was employed to optimize these selected parameters. The optimal activity was obtained at cellulose concentration 19.7 g/L and pH of 7.2. Under the optimized conditions, CMCase activity was 83.63 ± 1.86 IU/mL and filter paper activity was 2.58 ± 0.2 filter paper units per mL. Enzyme productivity was higher compared to previous reports. The enzyme produced from T. reesei was concentrated and was evaluated for deinking of printed paper, which demonstrated the suitability of the enzyme for this application.

Keywords: cellulase; Trichoderma; endoglucanase; deinking; paper and pulp; response surface

Acknowledgements

ASOI gratefully acknowledges the financial assistance provided by Department of Biotechnology (DBT), Government of India, and The World Academy of Sciences for the Advancement of Science in developing countries (TWAS) in the form of fellowship for supporting his PhD program, of which this study forms a part.

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Abbreviations
ANOVA: analysis of variance
CCD: central composite design
CMC: carboxymethyl cellulose
CMCase: carboxymethyl cellulase
CSL: corn steep liquor
DAHP: diammonium hydrogenphosphate
DNS: 3’5’-dinitrosalicylic acid
IU: international unit

Received: 2015-12-31

Accepted: 2016-3-19

Published Online: 2016-4-20

Published in Print: 2016-3-1

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https://doi.org/10.1515/biolog-2016-0046

Schlagwörter für diesen Artikel

cellulase; Trichoderma; endoglucanase; deinking; paper and pulp; response surface