Evaluation of the Bio-Rad VARIANT™ II HbA 2/HbA 1C Dual Program for measurement of hemoglobin concentrations and detection of variants
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Jean Riou
Abstract
In this work data obtained on the VARIANT™ II hemoglobin analyzer using the Dual Kit elution system were compared to those obtained with the β-Thalassemia Short Program. Since many laboratories still use an earlier model of the hemoglobin analyzer, the Variant™ 1, these data were also compared to those obtained with the latter instrument. Our study is divided into two parts. The first is an evaluation of the precision of the VARIANT™ II for determining the levels of hemoglobin (Hb)A 2 and Hb F. This was carried out for normal subjects, thalassemic patients and patients with HbS. The second part concerns the potential of this instrument in the presumptive identification of Hb variants.
References
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©2005 by Walter de Gruyter Berlin New York
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- Quality control for SELDI analysis
- Immunobead multiplex RT-PCR detection of carcinoembryonic genes expressing cells in the blood of colorectal cancer patients
- Optimization and evaluation of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) with reversed-phase protein arrays for protein profiling
- Simultaneous determination of HIV antibodies, hepatitis C antibodies, and hepatitis B antigens in dried blood spots –a feasibility study using a multi-analyte immunoassay
- Hematopoietic cytokines in the sera of patients with pancreatic cancer
- Modulation of translation factor's gene expression by histone deacetylase inhibitors in breast cancer cells
- Whole genome amplification of buccal cell DNA: genotyping concordance before and after multiple displacement amplification
- Y-Chromosome short tandem repeat (STR) haplotypes in a Campania population sample
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- Selective measurement of HCHO in urine using direct liquid-phase fluorimetric analysis
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- Measurement of reticulocyte and red blood cell indices in patients with iron deficiency anemia and β-thalassemia minor
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