Selective measurement of HCHO in urine using direct liquid-phase fluorimetric analysis
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Luke Chandler Short
Abstract
Quantification of formaldehyde (HCHO) in urine was recently shown to be a promising tool in the investigation of cancer, particularly bladder cancer. Development of a low-maintenance, inexpensive and rapid analyzer for HCHO in urine would greatly facilitate future research and the potential diagnosis of bladder cancer. We examine here the application of an off-the-shelf system, originally designed for gas-phase atmospheric monitoring of HCHO, for the quantification of HCHO in urine. Under strict dietary protocols, e.g., avoidance of foods rich in free or chemically bound HCHO, an increase in HCHO in urine is an indirect indicator of cancer in the urogenital system. The concentration of HCHO in urine samples from an individual over a several-month period was determined, with a range from 39 to 1400μM and a mean of 600μM. The limit of detection for the present method was 0.1μM. The proposed technique provides a direct, low-cost and greatly simplified analytical method for the quantification of HCHO in urine compared to other available techniques.
References
1 Maruyama R, Toyooka S, Toyooka KO, Virmani AK, Zochbauer-Muller S, Farinas AJ, et al. Aberrant promoter methylation profile of prostate cancers and its relationship to clinicopathological features. Clin Cancer Res 2002; 8: 514–9. Search in Google Scholar
2 Spanel P, Smith S, Holland T, Singary W, Elder J. Analysis of formaldehyde in the headspace of urine from bladder and prostate cancer patients using selected ion flow tube mass spectrometry. Rapid Commun Mass Spectrom, 1999; 13: 1354–9. 10.1002/(SICI)1097-0231(19990730)13:14<1354::AID-RCM641>3.0.CO;2-JSearch in Google Scholar
3 Phillips M, Welt S, Andrzejewski J, Liederson S, Masood SK, Alam M, et al. Volatile organic metabolites of breast cancer cells. J Invest Med 1996; 44: A417. Search in Google Scholar
4 Kato S, Burke P, Fenick D, Taatjes D, Bierbaum V, Koch T. Mass spectrometric measurement of formaldehyde generated in breast cancer cells upon treatment with anthracycline antitumor drugs. Chem Res Toxicol 2000; 13: 509–16. 10.1021/tx000008mSearch in Google Scholar
5 Ebeler S, Clifford A, Shibamoto T. Quantitative analysis by gas chromatography of volatile carbonyl compounds in expired air from mice and human. J Chromatogr B Biomed Sci Appl 1997; 702: 211–5. 10.1016/S0378-4347(97)00369-1Search in Google Scholar
6 Kato S, Burke P, Koch T, Bierbaum V. Formaldehyde in human cancer cells: detection by preconcentration-chemical ionization mass spectrometry. Anal Chem 2001; 73: 2992–7. 10.1021/ac001498qSearch in Google Scholar
7 Leach RA, Tuck MT. Methionine depletion induces transcription of the mRNA (N6-adenosine)methyltransferase. Int J Biochem Cell Biol 2001; 33: 1116–28. 10.1016/S1357-2725(01)00072-3Search in Google Scholar
8 Huszti Z, Tyihak E. Formation of formaldehyde from S-adenosyl-L-[methyl-3H]methionine during enzymic transmethylation of histamine. FEBS Lett 1986; 209: 362–6. 10.1016/0014-5793(86)81143-7Search in Google Scholar
9 Tyihák E, Trezl L, Szende B. Formaldehyde cycle and the phases of stress syndrome. Ann NY Acad Sci 1998; 851: 259–70. 10.1111/j.1749-6632.1998.tb09001.xSearch in Google Scholar
10 Ebeler SE, Hinrichs SH, Clifford AJ, Shibamoto T. Volatile carbonyl levels in tissues of transgenic mice with nerve sheath tumors. J Chromatogr Biomed Appl 1994; 654: 9–18. 10.1016/0378-4347(93)E0436-TSearch in Google Scholar
11 Deng Y, Yu P. Simultaneous determination of formaldehyde and methylglyoxal in urine: involvement of semicarbazide-sensitive amine oxidase-mediated deamination in diabetic complications. J Chromatogr Sci 1999; 37: 317–22. 10.1093/chromsci/37.9.317Search in Google Scholar
12 Shara MA, Dickson PH, Bagchi D, Stohs SJ. Excretion of formaldehyde, malondialdehyde, acetaldehyde and acetone in the urine of rats in response to 2,3,7,8-tetrachlorodibenzo-para-dioxin, paraquat, endrin and carbon tetrachloride. J Chromatogr Biomed 1992; 576: 221–33. 10.1016/0378-4347(92)80196-WSearch in Google Scholar
13 Smith D, Spanel P, Holland TA, Singari W, Elder JB. Selected ion flow tube mass spectrometry of urine headspace. Rapid Commun Mass Spectrom 1999; 13: 724–9. 10.1002/(SICI)1097-0231(19990430)13:8<724::AID-RCM548>3.0.CO;2-ESearch in Google Scholar
14 Nash T. Citation classic – the colorimetric estimation of formaldehyde by means of the Hantzsch Reaction. Curr Contents Life Sci 1981: 14. Search in Google Scholar
15 Dasgupta P, Dong S, Hwang H, Yang H, Genfa Z. Continuous liquid-phase fluorometry coupled to a diffusion scrubber for the real-time determination of atmospheric formaldehyde, hydrogen peroxide, and sulfur dioxide. Atmos Environ 1988; 22: 949–64. 10.1016/0004-6981(88)90273-9Search in Google Scholar
16 Dong S, Dasgupta PK. Fast fluorometric flow-injection analysis of formaldehyde in atmospheric water. Environ Sci Technol 1987; 21: 581–8. 10.1021/es00160a009Search in Google Scholar
17 Nash T. The colorimetric estimation of formaldehyde by means of the Hantzsch Reaction. Biochem J 1953; 55: 416–21. 10.1042/bj0550416Search in Google Scholar
18 Betterton E, Hoffman M. Henry's Law constants of some environmentally important aldehydes. Environ Sci Technol 1988; 22: 1415–8. 10.1021/es00177a004Search in Google Scholar
19 Staudinger J, Roberts PV. A critical compilation of Henry's Law constant temperature dependence relations for organic compounds in dilute aqueous solutions. Chemosphere 2001; 44: 561–76. 10.1016/S0045-6535(00)00505-1Search in Google Scholar
20 Staudinger J, Roberts P. A critical review of Henry's Law constants for environmental applications. Crit Rev Environ Sci Technol 1996; 26: 205–97. 10.1080/10643389609388492Search in Google Scholar
21 Petersen H, Petri N. Formaldehyde – general situation, test reactions, application in high-grade textile finishing. Melliand Textilber Int Textile Rep 1985; 66: 217–22. Search in Google Scholar
22 Strom J, Jun H. Separation and quantitation of methenamine in urine by ion-pair extraction. J Pharm Sci 1986; 75: 416–20. 10.1002/jps.2600750422Search in Google Scholar
©2005 by Walter de Gruyter Berlin New York
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- Quality control for SELDI analysis
- Immunobead multiplex RT-PCR detection of carcinoembryonic genes expressing cells in the blood of colorectal cancer patients
- Optimization and evaluation of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) with reversed-phase protein arrays for protein profiling
- Simultaneous determination of HIV antibodies, hepatitis C antibodies, and hepatitis B antigens in dried blood spots –a feasibility study using a multi-analyte immunoassay
- Hematopoietic cytokines in the sera of patients with pancreatic cancer
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- Whole genome amplification of buccal cell DNA: genotyping concordance before and after multiple displacement amplification
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- Selective measurement of HCHO in urine using direct liquid-phase fluorimetric analysis
- A spectrophotometric micromethod for determining erythrocyte protoporphyrin-IX in whole blood or erythrocytes
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- Poor knowledge and faulty thinking regarding hemolysis and potassium elevation
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- Evaluation of a novel semi-automated HPLC procedure for whole blood cyclosporin A confirms equivalence to adjusted monoclonal values from Abbott TDx
- Evaluation of the Bio-Rad VARIANT™ II HbA 2/HbA 1C Dual Program for measurement of hemoglobin concentrations and detection of variants
- Interferences in coagulation tests – evaluation of the 570-nm method on the Dade Behring BCS analyser
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