Home Hematopoietic cytokines in the sera of patients with pancreatic cancer
Article
Licensed
Unlicensed Requires Authentication

Hematopoietic cytokines in the sera of patients with pancreatic cancer

  • Barbara Mroczko , Maciej Szmitkowski , Urszula Wereszczyńska-Siemiątkowska and Grażyna Jurkowska
Published/Copyright: June 1, 2005
Become an author with De Gruyter Brill
Submit Manuscript Author Information
Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 43 Issue 2

Abstract

Hematopoietic cytokines (HCs) can affect the growth and spread of cancer. Therefore, in the present study, we investigated in pancreatic cancer patients the serum levels of selected HCs, such as stem cell factor (SCF), interleukin 3 (IL-3), granulocyte-macrophage-colony stimulating factor (GM-CSF), granulocyte-colony stimulating factor (G-CSF) and macrophage-colony stimulating factor (M-CSF) in relation to a control group and to a group of patients with chronic pancreatitis. Classical tumor markers such as carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA 19-9) were also tested. We compared the serum level of cytokines with the tumor stage. The diagnostic sensitivity, specificity, positive and negative predictive values and receiver-operating characteristics (ROC) curve for cytokines and classical tumor markers were defined. The cytokines were measured in 48 patients with pancreatic cancer, in 23 patients with chronic pancreatitis and in 40 healthy subjects. HCs were determined using ELISA. CEA and CA 19-9 were measured by microparticle enzyme immunoassay. There were significant differences in the levels of circulating SCF, IL-3, GM-CSF, M-CSF, CEA and CA 19-9 in the pancreatic cancer patients compared to the control group. The serum levels of M-CSF and tumor markers were significantly higher in pancreatic cancer patients compared to the pancreatitis group. The levels of SCF, M-CSF and tumor markers were higher in patients with a more advanced tumor stage. The M-CSF serum levels in the pancreatitis group correlated positively with the tumor markers tested – CEA and CA 19-9. The diagnostic sensitivity of SCF and specificity of M-CSF and tumor markers were the highest. The SCF and M-CSF areas under the ROC curve were greater than the areas for other cytokines. These results suggest the potential usefulness of HCs in pancreatic cancer detection; however, further investigations of early-stage pancreatic cancer patients and confirmation by a prospective study are necessary.

Keywords: hematopoietic cytokines; pancreatic cancer
Corresponding author: M. Szmitkowski MD, PhD, Department of Biochemical Diagnostics, Medical Academy, M. Skłodowska-Curie 24A, 15-276 Białystok, Poland Phone: +48-85-7468587, Fax: +48-85-7468585,

References

1 Tobita K, Kijima H, Dowaki S, Kashiwagi H, Ohtani Y, Oida Y, et al. Epidermal growth factor receptor expression in human pancreatic cancer: significance for liver metastasis. Int J Mol Med 2003; 11: 305–9. 10.3892/ijmm.11.3.305Search in Google Scholar

2 Bardeesy N, DePinho RA. Pancreatic cancer biology and genetic. Cancer 2002; 2: 897–909. Search in Google Scholar

3 Uematsu T, Tsuchie K, Ukai K, Kimoto E, Funakawa T, Mizuno R. Granulocyte-colony stimulating factor produced by pancreatic carcinoma. Int J Pancr 1996; 9: 135–9. 10.1007/BF02805227Search in Google Scholar

4 Dunlop RJ, Campbell CW. Cytokines and advanced cancer. J Pain Sympt Manage 2000; 20: 214–32. 10.1016/S0885-3924(00)00199-8Search in Google Scholar

5 Esposito I, Kleeff J, Bischoff SC, Fischer L, Collecchi P, Iorio M, et al. The stem cell factor-c-kit system and mast cells in human pancreatic cancer. Lab Invest 2002; 82: 1481–92. 10.1097/01.LAB.0000036875.21209.F9Search in Google Scholar PubMed

6 McDermott RS, Deneux L, Mosseri V, Vedrenne J, Clough K, Fourquet A, et al. Circulating macrophage colony stimulating factor as a marker of tumor progression. Eur Cytokine Netw 2002; 13: 121–7. Search in Google Scholar

7 Pei XH, Nakanishi Y, Takayama K, Bai F, Hara N. Granulocyte, granulocyte-macrophage, and macrophage colony-stimulating factors can stimulate the invasive capacity of human lung cancer cells. Br J Cancer 1999; 79: 40–6. 10.1038/sj.bjc.6690009Search in Google Scholar PubMed PubMed Central

8 Mroczko B, Szmitkowski M, Okulczyk B, Piotrowski Z. Granulocyte-macrophage-colony stimulating factor in patients with colorectal cancer. Folia Hist Cytobiol 2001; 39(Suppl 2): 110–1. 10.1515/CCLM.2001.059Search in Google Scholar PubMed

9 Mroczko B, Szmitkowski M, Okulczyk B. Granulocyte-colony stimulating factor (G-CSF) and macrophage-colony stimulating factor (M-CSF) in colorectal cancer patients. Clin Chem Lab Med 2002; 40: 351–5. 10.1515/CCLM.2002.056Search in Google Scholar PubMed

10 Mroczko B, Szmitkowski M, Okulczyk B. Hematopoietic growth factors in colorectal cancer patients. Clin Chem Lab Med 2003; 41: 646–51. 10.1515/CCLM.2003.098Search in Google Scholar PubMed

11 Mroczko B, Szmitkowski M, Wereszczynska-Siemiatkowska U, Jurkowska G. Stem cell factor and macrophage-colony stimulating factor in patients with pan- creatic cancer. Clin Chem Lab Med 2004; 42: 256–60. 10.1515/CCLM.2004.047Search in Google Scholar PubMed

12 Rosch T. The new TNM classification in gastroenterology (1997). Endoscopy 1998; 30: 643–9. Search in Google Scholar

13 Kairisto V, Virtanen A, Uusipaikka E, Voipio-Pulkki LM, Nanto V, Peltola O, et al. Method for determining reference changes from patients serial data: example of cardiac enzymes. Clin Chem 1993; 39: 2298–304. 10.1093/clinchem/39.11.2298Search in Google Scholar

14 Parker SL, Tong T, Bolden S, Wingo PA. Cancer statistics. CA Cancer J Clin 1997; 47: 5–27. 10.3322/canjclin.47.1.5Search in Google Scholar

15 Nemunaitis J. A comparative review of colony-stimula ting factors. Drugs 1997; 54: 709–29. 10.2165/00003495-199754050-00004Search in Google Scholar

16 Trutmann M, Terracciano L, Noppen C, Kloth J, Kaspar M, Peterli R, et al. GM-CSF gene expression and protein production in human colorectal cell lines and clinical tumor specimens. Int J Cancer 1998; 77: 378–85. 10.1002/(SICI)1097-0215(19980729)77:3<378::AID-IJC12>3.0.CO;2-4Search in Google Scholar

17 Young MR, Lozano Y, Coogan M. Stimulation of the metastasis properties of Lewis-lung-carcinoma cells by autologous granulocyte macrophage colony-stimula- ting factor. Int J Cancer 1992; 50: 628–34. 10.1002/ijc.2910500424Search in Google Scholar

18 Chambers SK, Wang Y, Gertz RE, Kacinski BM. Macrophage colony-stimulating factor mediates invasion of ovarian cancer cells through urokinase. Cancer Res 1995; 55: 1578–85. Search in Google Scholar

19 Mroczko B, Szmitkowski M, Niklinski J. Stem cell factor and granulocyte-macrophage-colony stimulating factor as candidates for tumour markers for non-small-cell lung cancer. Clin Chem Lab Med 1999; 37: 959–62. 10.1515/CCLM.1999.141Search in Google Scholar

20 Mroczko B, Szmitkowski M, Niklinski J. Granulocyte-colony stimulating factor and macrophage-colony stimulating factor in patients with non-small-cell lung cancer. Clin Chem Lab Med 2001; 39: 374–9. 10.1515/CCLM.2001.059Search in Google Scholar

Received: 2004-7-5
Accepted: 2004-12-14
Published Online: 2005-6-1
Published in Print: 2005-4-1

©2005 by Walter de Gruyter Berlin New York

Articles in the same Issue

  1. Quality control for SELDI analysis
  2. Immunobead multiplex RT-PCR detection of carcinoembryonic genes expressing cells in the blood of colorectal cancer patients
  3. Optimization and evaluation of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) with reversed-phase protein arrays for protein profiling
  4. Simultaneous determination of HIV antibodies, hepatitis C antibodies, and hepatitis B antigens in dried blood spots –a feasibility study using a multi-analyte immunoassay
  5. Hematopoietic cytokines in the sera of patients with pancreatic cancer
  6. Modulation of translation factor's gene expression by histone deacetylase inhibitors in breast cancer cells
  7. Whole genome amplification of buccal cell DNA: genotyping concordance before and after multiple displacement amplification
  8. Y-Chromosome short tandem repeat (STR) haplotypes in a Campania population sample
  9. TaqMan assays for genotyping of single nucleotide polymorphisms present at a disease susceptibility locus on chromosome 6
  10. The secretion of ibuprofen metabolites interferes with the capillary chromatography of urinary homovanillic acid and 4-hydroxy-3-methoxymandelic acid in neuroblastoma diagnosis
  11. Selective measurement of HCHO in urine using direct liquid-phase fluorimetric analysis
  12. A spectrophotometric micromethod for determining erythrocyte protoporphyrin-IX in whole blood or erythrocytes
  13. Simultaneous analysis of MDR1 C3435T, G2677T/A, and C1236T genotypes by multiplexed mutagenically separated PCR
  14. Measurement of reticulocyte and red blood cell indices in patients with iron deficiency anemia and β-thalassemia minor
  15. Chitotriosidase activity in colostrum from African and Caucasian women
  16. Integration between point-of-care cardiac markers in an emergency/cardiology department and the central laboratory: methodological and preliminary clinical evaluation
  17. Controlled storage conditions prolong stability of biochemical components in whole blood
  18. Poor knowledge and faulty thinking regarding hemolysis and potassium elevation
  19. Delayed effects of short-term transdermal application of 7-oxo-dehydroepiandrosterone on its metabolites, some hormonal steroids and relevant proteohormones in healthy male volunteers
  20. Evaluation of a novel semi-automated HPLC procedure for whole blood cyclosporin A confirms equivalence to adjusted monoclonal values from Abbott TDx
  21. Evaluation of the Bio-Rad VARIANT™ II HbA 2/HbA 1C Dual Program for measurement of hemoglobin concentrations and detection of variants
  22. Interferences in coagulation tests – evaluation of the 570-nm method on the Dade Behring BCS analyser
  23. No evidence for involvement of the human inducible nitric oxide synthase gene in susceptibility to coronary artery disease
  24. National survey on the use of measurement of cholinesterase activity in serum
https://doi.org/10.1515/CCLM.2005.024
Keywords for this article
hematopoietic cytokines; pancreatic cancer

Articles in the same Issue

  1. Quality control for SELDI analysis
  2. Immunobead multiplex RT-PCR detection of carcinoembryonic genes expressing cells in the blood of colorectal cancer patients
  3. Optimization and evaluation of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) with reversed-phase protein arrays for protein profiling
  4. Simultaneous determination of HIV antibodies, hepatitis C antibodies, and hepatitis B antigens in dried blood spots –a feasibility study using a multi-analyte immunoassay
  5. Hematopoietic cytokines in the sera of patients with pancreatic cancer
  6. Modulation of translation factor's gene expression by histone deacetylase inhibitors in breast cancer cells
  7. Whole genome amplification of buccal cell DNA: genotyping concordance before and after multiple displacement amplification
  8. Y-Chromosome short tandem repeat (STR) haplotypes in a Campania population sample
  9. TaqMan assays for genotyping of single nucleotide polymorphisms present at a disease susceptibility locus on chromosome 6
  10. The secretion of ibuprofen metabolites interferes with the capillary chromatography of urinary homovanillic acid and 4-hydroxy-3-methoxymandelic acid in neuroblastoma diagnosis
  11. Selective measurement of HCHO in urine using direct liquid-phase fluorimetric analysis
  12. A spectrophotometric micromethod for determining erythrocyte protoporphyrin-IX in whole blood or erythrocytes
  13. Simultaneous analysis of MDR1 C3435T, G2677T/A, and C1236T genotypes by multiplexed mutagenically separated PCR
  14. Measurement of reticulocyte and red blood cell indices in patients with iron deficiency anemia and β-thalassemia minor
  15. Chitotriosidase activity in colostrum from African and Caucasian women
  16. Integration between point-of-care cardiac markers in an emergency/cardiology department and the central laboratory: methodological and preliminary clinical evaluation
  17. Controlled storage conditions prolong stability of biochemical components in whole blood
  18. Poor knowledge and faulty thinking regarding hemolysis and potassium elevation
  19. Delayed effects of short-term transdermal application of 7-oxo-dehydroepiandrosterone on its metabolites, some hormonal steroids and relevant proteohormones in healthy male volunteers
  20. Evaluation of a novel semi-automated HPLC procedure for whole blood cyclosporin A confirms equivalence to adjusted monoclonal values from Abbott TDx
  21. Evaluation of the Bio-Rad VARIANT™ II HbA 2/HbA 1C Dual Program for measurement of hemoglobin concentrations and detection of variants
  22. Interferences in coagulation tests – evaluation of the 570-nm method on the Dade Behring BCS analyser
  23. No evidence for involvement of the human inducible nitric oxide synthase gene in susceptibility to coronary artery disease
  24. National survey on the use of measurement of cholinesterase activity in serum
Sign up now to receive a 20% welcome discount
Institutional Access
How does access work?
Have an idea on how to improve our website?
Please write us.
© 2025 De Gruyter Brill
Downloaded on 29.10.2025 from https://www.degruyterbrill.com/document/doi/10.1515/CCLM.2005.024/html
Scroll to top button