Simultaneous determination of HIV antibodies, hepatitis C antibodies, and hepatitis B antigens in dried blood spots –a feasibility study using a multi-analyte immunoassay
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Zoltan Lukacs
Abstract
HIV in particular, as well as hepatitis B and C, present a burden on healthcare systems worldwide. Early detection of these diseases may prevent further infections and improve the outcome for patients. In particular, transmission of HIV from mother to child can be significantly reduced when preventive measures are taken before birth. We have developed and optimized a method for the simultaneous detection of HIV 1 and hepatitis B and C from dried blood specimensusing the Luminex multi-analyte profiling technology (LabMap). Dried blood spots provide a convenient method for mailing, analysis and storage of samples. Specimens from known HIV-positive children (n=46) as well as hepatitis B- (n=8) and hepatitis C-positive patients (n=7) tested positive in our assay. Storage for up to 10years did not interfere with the test in the case of HIV-positive patients. Results for five different antibodies and one antigen were obtained in approximately 80seconds. Furthermore, antibody levels in infants of HIV-positive mothers were monitored over a period of 1year. Antibodies were no longer detectable after 260–360days, which compared well with results independently obtained by ELISA and Western blot analysis. We demonstrated the feasibility of the simultaneous detection of infectious diseases from dried blood. Our novel method also provides a convenient tool for monitoring children from HIV-positive mothers and for possible screening efforts.
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©2005 by Walter de Gruyter Berlin New York
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- Quality control for SELDI analysis
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- Optimization and evaluation of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) with reversed-phase protein arrays for protein profiling
- Simultaneous determination of HIV antibodies, hepatitis C antibodies, and hepatitis B antigens in dried blood spots –a feasibility study using a multi-analyte immunoassay
- Hematopoietic cytokines in the sera of patients with pancreatic cancer
- Modulation of translation factor's gene expression by histone deacetylase inhibitors in breast cancer cells
- Whole genome amplification of buccal cell DNA: genotyping concordance before and after multiple displacement amplification
- Y-Chromosome short tandem repeat (STR) haplotypes in a Campania population sample
- TaqMan assays for genotyping of single nucleotide polymorphisms present at a disease susceptibility locus on chromosome 6
- The secretion of ibuprofen metabolites interferes with the capillary chromatography of urinary homovanillic acid and 4-hydroxy-3-methoxymandelic acid in neuroblastoma diagnosis
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- A spectrophotometric micromethod for determining erythrocyte protoporphyrin-IX in whole blood or erythrocytes
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- Poor knowledge and faulty thinking regarding hemolysis and potassium elevation
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