Measurement of reticulocyte and red blood cell indices in patients with iron deficiency anemia and β-thalassemia minor
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José F. A. Noronha
Abstract
New parameters correlated with the hemoglobin content in reticulocytes (RET-Y) and in red blood cells (RBC-Y) have been suggested as helpful in diagnosing iron deficiency anemia. We have studied RET-Y and RBC-Y indices in two groups of patients with microcytosis to verify if these parameters could be used to differentiate iron deficiency anemia from β-thalassemia minor. Blood samples from 33 iron-deficient patients, 25 β-thalassemic minor patients and 50 normal individuals were analyzed on a Sysmex XE-2100 instrument. A significant difference was observed in reticulocyte counting and immature reticulocyte fraction between iron deficiency anemia and β-thalassemia minor groups, but not in RBC-X and RET-Y parameters. Reticulocyte counting was higher in β-thalassemia minor and the immature reticulocyte fraction was higher in severe iron deficiency anemia. The ratio RET-Y/mean cell volume was tested and was significantly different when β-thalassemia minor was compared with mild and severe iron deficiency anemia, and showed better performance than the Mentzer ratio and the Green and King function. A great overlap of RET-Y and RBC-Y individual values was observed in both groups of microcytic anemias; we conclude that these new indices may be used with caution as indicative of iron deficiency, mainly in populations where β-thalassemia minor is frequent.
References
1 Franck S, Linssen J, Messinger M, Thomas L. Potential utility of Ret-Y in the diagnosis of iron-restricted erythropoiesis. Clin Chem 2004; 50: 1240–2. 10.1373/clinchem.2004.030254Suche in Google Scholar PubMed
2 Briggs C, Rogers R, Thompson B, Machin SJ. New red cell parameters on the Sysmex XE-2100 as potential markers of functional iron deficiency. Infus Ther Transfus Med 2001; 28: 256–62. 10.1159/000050250Suche in Google Scholar
3 Green R, King R. A new red cell discriminant incorporating volume for differentiating iron deficiency from thalassemia minor. Blood Cells 1989; 15: 481–95. Suche in Google Scholar
4 Mentzer Jr WC. Differentiation of iron deficiency from thalassemia trait. Lancet 1983; 1: 882. Suche in Google Scholar
5 Tassiopoulos T, Konstantopoulos K, Tassiopolulos S, Rombos Y, Alevizou-Terzaki V, Kyriaki P, et al. Erythropoietin levels and microcytosis in heterozygous beta-thalassaemia. Acta Haematol 1997; 98: 147–9. 10.1159/000203609Suche in Google Scholar PubMed
6 Buttarello M, Temporin V, Ceravolo R, Farina G, Bulian P. The new reticulocyte parameter (RET-Y) of the Sysmex XE 2100. Am J Clin Pathol 2004; 121: 489–95. 10.1309/W65295DTUWK7U1HHSuche in Google Scholar
7 Brugnara C. Reticulocyte cellular indices: a new approach in the diagnosis of anemias and monitoring of erythropoietic function. Clin Lab Sci 2000; 37: 93–130. 10.1080/10408360091174196Suche in Google Scholar PubMed
8 Skarmoutsou C, Papassotiriou I, Traeger-Synodinos J, Stamou H, Ladis V, Metaxotou-Mavrommati A, et al. Erythroid bone marrow activity and red cell hemoglobinization in iron-sufficient β-thalassemia heterozygous as reflected by soluble transferrin receptor and reticulocyte hemoglobin content. Correlation with genotypes and HbA 2 levels. Haematologica 2003; 88: 631–6. Suche in Google Scholar
©2005 by Walter de Gruyter Berlin New York
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- Quality control for SELDI analysis
- Immunobead multiplex RT-PCR detection of carcinoembryonic genes expressing cells in the blood of colorectal cancer patients
- Optimization and evaluation of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) with reversed-phase protein arrays for protein profiling
- Simultaneous determination of HIV antibodies, hepatitis C antibodies, and hepatitis B antigens in dried blood spots –a feasibility study using a multi-analyte immunoassay
- Hematopoietic cytokines in the sera of patients with pancreatic cancer
- Modulation of translation factor's gene expression by histone deacetylase inhibitors in breast cancer cells
- Whole genome amplification of buccal cell DNA: genotyping concordance before and after multiple displacement amplification
- Y-Chromosome short tandem repeat (STR) haplotypes in a Campania population sample
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