Autoantibodies against Oxidized Low-Density Lipoprotein Correlate with Achilles Tendon Xanthomas
-
Masahiko Okada
Abstract
Although there is increasing evidence for anti-oxidized low-densitiy lipoprotein (LDL) autoantibodies in human sera, their diagnostic utility remains controversial. We examined the difference in autoantibody titers between patients with Achilles tendon xanthoma and control subjects. Fifteen hyperlipidemic patients with Achilles tendon xanthoma (group A+) and 94 hyperlipidemic patients without Achilles tendon xanthoma (group A−) were studied. Quantification of anti-oxidized LDL and anti-native LDL autoantibodies was performed using an ELISA method. To calculate antibody titers, we used the ratio between the spectrophotometric reading of anti-oxidized LDL and anti-native LDL wells. Using oxidized LDL that was purified by gel-permeation chromatography as antigen, immunoglobulin G level differed significantly between groups A+ and A− (p ＜ 0.01). In contrast, using native and oxidized LDL as antigens without chromatographical purification revealed no significant difference between the two groups. Furthermore, immunoglobulin autoantibody titer did not correlate with age, body mass index, total cholesterol, high-density lipoprotein cholesterol, LDL cholesterol, or triglyceride in the entire group of subjects. Thus, immunoglobulin G autoantibody values appear to correlate with Achilles tendon xanthoma.
Copyright © 2000 by Walter de Gruyter GmbH & Co. KG
Artikel in diesem Heft
- Integrons: an Antibiotic Resistance Gene Capture and Expression System
- Levels of C-Telopeptide Fragments of Collagen Type I Enable the Monitoring and Early Adjustment of Clodronate Therapy in Patients with Postmenopausal Osteoporosis
- Probable Involvement of Cathepsin D in the Degradation of β2-Microglobulin in Acidic Urine
- Autoantibodies against Oxidized Low-Density Lipoprotein Correlate with Achilles Tendon Xanthomas
- Regulation of the Hepatic Endothelin System in Advanced Biliary Fibrosis in Rats
- Determination of Plasma Methoxyamines
- Stability of Brain Natriuretic Peptide (BNP) in Human Whole Blood and Plasma
- Effect of Short- and Long-Term Storage on Human Serum and Recombinant Apolipoprotein E Concentration
- Comparison of Laboratory Parameters as Risk Factors for the Presence and the Extent of Coronary or Carotid Atherosclerosis: the Significance of Apolipoprotein B to Apolipoprotein AII Ratio
- The Effect of a Gender Difference in the Apolipoprotein E Gene DNA Polymorphism on Serum Lipid Levels in a Serbian Healthy Population
- A Model for Setting Analytical Quality Specifications and Design of Control for Measurements on the Ordinal Scale
- Transformation in the PC-Aided Biochemical Data Analysis
- Concordance of Eight Kits for Antithyroid Peroxidase Autoantibodies Determination
- Evaluation of a Point-of-Care System for Quantitative Determination of Troponin T and Myoglobin
- The Maillard Reaction in Foods and Medicine. By John OBrien, Harry E. Nursden, M. James, C. Crabbe and Jennifer M. Ames, editors
- Apoptosis and Inflammation. By J.D. Winkler, editor
Artikel in diesem Heft
- Integrons: an Antibiotic Resistance Gene Capture and Expression System
- Levels of C-Telopeptide Fragments of Collagen Type I Enable the Monitoring and Early Adjustment of Clodronate Therapy in Patients with Postmenopausal Osteoporosis
- Probable Involvement of Cathepsin D in the Degradation of β2-Microglobulin in Acidic Urine
- Autoantibodies against Oxidized Low-Density Lipoprotein Correlate with Achilles Tendon Xanthomas
- Regulation of the Hepatic Endothelin System in Advanced Biliary Fibrosis in Rats
- Determination of Plasma Methoxyamines
- Stability of Brain Natriuretic Peptide (BNP) in Human Whole Blood and Plasma
- Effect of Short- and Long-Term Storage on Human Serum and Recombinant Apolipoprotein E Concentration
- Comparison of Laboratory Parameters as Risk Factors for the Presence and the Extent of Coronary or Carotid Atherosclerosis: the Significance of Apolipoprotein B to Apolipoprotein AII Ratio
- The Effect of a Gender Difference in the Apolipoprotein E Gene DNA Polymorphism on Serum Lipid Levels in a Serbian Healthy Population
- A Model for Setting Analytical Quality Specifications and Design of Control for Measurements on the Ordinal Scale
- Transformation in the PC-Aided Biochemical Data Analysis
- Concordance of Eight Kits for Antithyroid Peroxidase Autoantibodies Determination
- Evaluation of a Point-of-Care System for Quantitative Determination of Troponin T and Myoglobin
- The Maillard Reaction in Foods and Medicine. By John OBrien, Harry E. Nursden, M. James, C. Crabbe and Jennifer M. Ames, editors
- Apoptosis and Inflammation. By J.D. Winkler, editor
