The Effect of a Gender Difference in the Apolipoprotein E Gene DNA Polymorphism on Serum Lipid Levels in a Serbian Healthy Population
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Sanja Stanković
Abstract
To date, no data have been available on relationship between apolipoprotein E polymorphism and lipid levels in Serbian populations. Blood samples were obtained from 591 healthy normal individuals (193 women and 398 men). A 244 bp sequence of the apolipoprotein E gene including the two polymorphic sites was amplified by polymerase chain reaction. After digestion with Hhal, DNA fragments were visualized by microplate array diagonal gel electrophoresis. In men, levels of both total and low-density lipoprotein cholesterol among the three apolipoprotein E genotype groups differed significantly (p <0.05). The ε2 allele was associated with lower concentrations of both total and low-density lipoprotein cholesterol, where the ε4 allele had the opposite effects. No significant effects of apolipoprotein E polymorphism on serum lipid levels were observed in women. The presented data could be taken into consideration in any future disease risk evaluation in this population.
Copyright © 2000 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Integrons: an Antibiotic Resistance Gene Capture and Expression System
- Levels of C-Telopeptide Fragments of Collagen Type I Enable the Monitoring and Early Adjustment of Clodronate Therapy in Patients with Postmenopausal Osteoporosis
- Probable Involvement of Cathepsin D in the Degradation of β2-Microglobulin in Acidic Urine
- Autoantibodies against Oxidized Low-Density Lipoprotein Correlate with Achilles Tendon Xanthomas
- Regulation of the Hepatic Endothelin System in Advanced Biliary Fibrosis in Rats
- Determination of Plasma Methoxyamines
- Stability of Brain Natriuretic Peptide (BNP) in Human Whole Blood and Plasma
- Effect of Short- and Long-Term Storage on Human Serum and Recombinant Apolipoprotein E Concentration
- Comparison of Laboratory Parameters as Risk Factors for the Presence and the Extent of Coronary or Carotid Atherosclerosis: the Significance of Apolipoprotein B to Apolipoprotein AII Ratio
- The Effect of a Gender Difference in the Apolipoprotein E Gene DNA Polymorphism on Serum Lipid Levels in a Serbian Healthy Population
- A Model for Setting Analytical Quality Specifications and Design of Control for Measurements on the Ordinal Scale
- Transformation in the PC-Aided Biochemical Data Analysis
- Concordance of Eight Kits for Antithyroid Peroxidase Autoantibodies Determination
- Evaluation of a Point-of-Care System for Quantitative Determination of Troponin T and Myoglobin
- The Maillard Reaction in Foods and Medicine. By John OBrien, Harry E. Nursden, M. James, C. Crabbe and Jennifer M. Ames, editors
- Apoptosis and Inflammation. By J.D. Winkler, editor
