Effect of Short- and Long-Term Storage on Human Serum and Recombinant Apolipoprotein E Concentration
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Françoise Schiele
Abstract
In order to assess the short- and long-term stability of apolipoprotein (apo) E concentration in serum, we compared the apo E concentrations measured in fresh human serum samples with those determined after storage at +4°C, −20°C or −80°C. The serum apo E concentration was measured by immunoturbidimetry using an anti-human apo E polyclonal antibody from goats. One week storage at +4°C did not significantly affect the serum apo E concentration. At −20°C or −80°C no significant change in apo E concentration occurred during up to three months of storage. Moreover, the concentration of apo E was not modified after long-term storage of serum samples kept at −196°C in liquid nitrogen for up to four years. In addition, 15 freeze-thaw cycles, over a 3-week period, did not affect the apo E concentration in serum. A similar freezethaw procedure applied to purified human recombinant apo E showed that apo E2 isoform was the most stable in comparison with the apo E3 and apo E4 isoforms.
Copyright © 2000 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Integrons: an Antibiotic Resistance Gene Capture and Expression System
- Levels of C-Telopeptide Fragments of Collagen Type I Enable the Monitoring and Early Adjustment of Clodronate Therapy in Patients with Postmenopausal Osteoporosis
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- Autoantibodies against Oxidized Low-Density Lipoprotein Correlate with Achilles Tendon Xanthomas
- Regulation of the Hepatic Endothelin System in Advanced Biliary Fibrosis in Rats
- Determination of Plasma Methoxyamines
- Stability of Brain Natriuretic Peptide (BNP) in Human Whole Blood and Plasma
- Effect of Short- and Long-Term Storage on Human Serum and Recombinant Apolipoprotein E Concentration
- Comparison of Laboratory Parameters as Risk Factors for the Presence and the Extent of Coronary or Carotid Atherosclerosis: the Significance of Apolipoprotein B to Apolipoprotein AII Ratio
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- The Maillard Reaction in Foods and Medicine. By John OBrien, Harry E. Nursden, M. James, C. Crabbe and Jennifer M. Ames, editors
- Apoptosis and Inflammation. By J.D. Winkler, editor
