Stability of Brain Natriuretic Peptide (BNP) in Human Whole Blood and Plasma
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Agnès Gobinet-Georges
Abstract
Brain natriuretic peptide is proposed as a biochemical marker which could provide a useful screening test to select patients for further cardiac investigations in heart failure. The applicability of such a biochemical test in clinics, hospital wards, and clinical laboratories is dependent on its ease of use and on the complexity of sample handling. The present study was undertaken to evaluate the stability of brain natriuretic peptide under a number of different handling conditions (sample collection, storage temperatures, freezing temperatures) assayed with a commercially available kit. The results clearly demonstrate that brain natriuretic peptide is stable at room temperature for 24 hours, or in up to 30°C for 12 hours in the presence and absence of aprotinin, on the condition that brain natriuretic peptide is assayed within one month (frozen at −20°C) after blood collection. The presence of aprotinin prevents brain natriuretic peptide degradation in samples preserved for more than 1 month at −20°C before assay.
Copyright © 2000 by Walter de Gruyter GmbH & Co. KG
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- Integrons: an Antibiotic Resistance Gene Capture and Expression System
- Levels of C-Telopeptide Fragments of Collagen Type I Enable the Monitoring and Early Adjustment of Clodronate Therapy in Patients with Postmenopausal Osteoporosis
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- Autoantibodies against Oxidized Low-Density Lipoprotein Correlate with Achilles Tendon Xanthomas
- Regulation of the Hepatic Endothelin System in Advanced Biliary Fibrosis in Rats
- Determination of Plasma Methoxyamines
- Stability of Brain Natriuretic Peptide (BNP) in Human Whole Blood and Plasma
- Effect of Short- and Long-Term Storage on Human Serum and Recombinant Apolipoprotein E Concentration
- Comparison of Laboratory Parameters as Risk Factors for the Presence and the Extent of Coronary or Carotid Atherosclerosis: the Significance of Apolipoprotein B to Apolipoprotein AII Ratio
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- The Maillard Reaction in Foods and Medicine. By John OBrien, Harry E. Nursden, M. James, C. Crabbe and Jennifer M. Ames, editors
- Apoptosis and Inflammation. By J.D. Winkler, editor
