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Characterization of electrospun fibrous scaffold produced from Indian eri silk fibroin

  • Muthumanickam Andiyappan , Subramanian Sundaramoorthy , Prasanna Vidyasekar , Natarajan Tirupattur Srinivasan and Rama Shanker Verma
Published/Copyright: August 22, 2013
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International Journal of Materials Research
From the journal International Journal of Materials Research Volume 104 Issue 5

Abstract

A scaffold, synthesized from bio-degradable polymers and Bombyxmori silk fibroin in the form of films and fibrous assemblies, has been used as the bio-material for in-vivo applications. In the present work, the scaffold was prepared from the fibroin of Indian eri silk via the electrospinning method. The diameter of the fibre produced was in the range of 300 to 900 nm. The scaffold was subjected to ethanol treatment to improve its dimensional stability, as there was the problem of curling and shrinking when it was treated with solutions used for the cell culture. The scaffold was characterized for surface, thermal and tensile properties. The dimensional stability of the scaffold improved and the porosity reduced, due to the treatment of the scaffold with ethanol. The average failure stress of the raw and ethanol treated scaffold was 2.34 MPa and 4.91 MPa respectively and the mean strain was 13.63 % and 7.91 % respectively. Bone marrow stromal cells were isolated from the bone marrow of Swiss albino mice, and cultured on the ethanol treated electrospun fibrous scaffold. Scanning electron microscopy of the culture was carried out to evaluate the attachment and growth of cells on the scaffold at different incubation periods. Mouse bone marrow stromal cells adhered and grew on the electrospun fibrous scaffold prepared from eri silk fibroin, and the cell density increased with increasing incubation periods.

Keywords: Eri silk fibroin electro spun fibre; FTIR; TGA; SEM; Tissue engineering
* Correspondence address, Dr. S. Subramanian, Associate Professor, Department of Textile Technology, Anna University Chennai, Tamil Nadu, Chennai - 600 025, India, Tel.: 91-44-22359247, E-mail:

References

[1] Y.-Z.Wang, H.-J.Kim, G.Vunjak-Novakovic, D.L.Kaplan: Biomaterials27 (2006) 6064. PMid: 16890988; 10.1016/j.biomaterials.2006.07.008Search in Google Scholar

[2] W.H.Park, W.S.Ha, H.Ito, T.Miyamoto, H.Inagaki, Y.Noishiki: Fibers and Polym.2 (2001) 58. 10.1007/BF02875259Search in Google Scholar

[3] S.Sukigara, M.Gandhi, J.Ayutsede, M.Micklus, F.Ko: Polymer44 (2003) 5721. 10.1016/S0032-3861(03)00532-9Search in Google Scholar

[4] M.K.Sah, K.Pramanik: Int. J. Environmental Sci. Developm.1 (5) (2010) 404.10.7763/IJESD.2010.V1.78Search in Google Scholar

[5] M.Santin, A.Motta, G.Freddi, M.Cannas: J. Biomed. Mater. Res.46 (1999) 382. 10.1002/(SICI)1097-4636(19990905)46:3<382::AID-JBM11>3.0.CO;2-RSearch in Google Scholar

[6] N.Minoura, S.Aiba, Y.Gotoh, M.Tsukada, Y.Imai: J. Biomed. Mater. Res.29 (10) (1995) 1215. PMid: 8557723; 10.1002/jbm.820291008Search in Google Scholar

[7] N.Minoura, S.Aiba, M.Higuchi, Y.Gotoh, M.Tsukada, Y.Imai: Biochem. Biophys. Res. Comm.208 (2) (1995) 511. PMid: 7695601; 10.1006/bbrc.1995.1368Search in Google Scholar

[8] C.Vepari, D.L.Kaplan: Prog. Polymer Sci.32 (2007) 991. PMid: 19543442; 10.1016/j.progpolymsci.2007.05.013Search in Google Scholar

[9] K.Inouye, M.Kurokawa, S.Nishikawa, M.Tsukada: J. Biochem. Biophys. Methods, 37 (3) (1998) 159. 10.1016/S0165-022X(98)00024-4Search in Google Scholar

[10] A.Sugihara, K.Sugiura, H.Morita, T.Ninagawa, K.TubouchiR.Tobe: Proc. Soc. Exp. Bio. Med.225 (1) (2000) 58. 10.1046/j.1525-1373.2000.22507.xSearch in Google Scholar

[11] L.Meinel, S.Hofmann, V.Karageorgiou, C.Kirker-Head, J.McCool, G.Gronowicz: Biomaterials26 (2) (2005) 147. PMid: 15207461; 10.1016/j.biomaterials.2004.02.047Search in Google Scholar

[12] G.Freddi, R.Mossott, R.Innocenti: J. Biotech.106 (2003) 101. 10.1016/j.jbiotec.2003.09.006Search in Google Scholar

[13] L.Meinel, S.Hofmann, V.Karageorgiou, C.Kirker-Head, J.McCool, G.Gronowicz: Biomaterials26 (2) (2005) 147. PMid: 15207461; 10.1016/j.biomaterials.2004.02.047Search in Google Scholar

[14] M.Santin, A.Motta, G.Freddi, M.Cannas: J. Biomed. Mater. Res.46 (1999) 382. 10.1002/(SICI)1097-4636(19990905)46:3<382::AID-JBM11>3.0.CO;2-RSearch in Google Scholar

[15] S.Prasong, S.Yaowalak, S.Wilaiwan: Pak. J. Bio. Sci.12 (11) (2009) 872. 10.3923/pjbs.2009.872.876Search in Google Scholar

[16] R.Ahmed, A.Kamra, S.E.Hasnain: DNA Cell Biol. 23 (3) (2004) 149. PMid: 15068584; 10.1089/104454904322964742Search in Google Scholar

[17] K.Sen, K. MurugeshBabu: J. App. Poly. Sci.92 (2004) 1080. 10.1002/app.13609Search in Google Scholar

[18] M.Li, W.Tao, S.Lu, C.Zhao: Polym. Adv. Technol.19 (2008) 207. 10.1002/pat.1018Search in Google Scholar

[19] H.W.Ouyang, J.C.H.Goh, A.Thambyah, S.H.Teoh, E.HLee: Tissue Eng.9 (2003) 431. PMid: 12857411; 10.1089/107632703322066615Search in Google Scholar

[20] B.-M.Min, G.Lee, S.H.Kim, Y.S.Nam, T.S.Le, W.H.Park: Biomaterials25 (2004) 1289. 10.1016/j.biomaterials.2003.08.045Search in Google Scholar

[21] J.Sanchez-Ramos, S.Song, F.Cardozo-Pelaez, C.Hazzi, T.Stedeford, A.Willing, T.B.Freeman, S.Saporta, W.Janssen, N.Patel, D.R.Cooper, R.Sanberg: Exp. Neurol.164 (2) (2000) 247. PMid: 10915564; 10.1006/exnr.2000.7389Search in Google Scholar

[22] R.Quarto, G.Bianchi, A.Derubeis, M.Mastrogiacomo, A.Muraglia, R.Cancedda: Euro Cells Mat. 4 (1) (2000) 28.Search in Google Scholar

[23] A.Muthumanickkam, E.Elankavi, R.Gayathri, Kubera S.Sampathkumar, G.Vijayakumar, K.Muthukumar, S.Subramanian: Int. J. Mater. Res.12 (2010) 1548. 10.3139/146.110429Search in Google Scholar

[24] N.Amiraliyan, M.Nouri, M. HaghighatKish: Polymer Sci. Series, A52 (4) (2010) 407. 10.1134/S0965545X10040097Search in Google Scholar

[25] K.Frost, D.Kaminski, G.Kirwan, E.Lascaris, R.Shanks: Carbohydrate Polym.78 (2009) 543. 10.1016/j.carbpol.2009.05.018Search in Google Scholar

[26] K.Whang, K.E.Healy, D.R.Elenz, E.K.Nam, D.C.Tsai, C.H.Thomas, G.W.Nuber, F.H.Glorieux, R.Travers, S.M.Sprague: Tissue Eng. 5 (1) (1999) 35. PMid: 10207188; 10.1089/ten.1999.5.35Search in Google Scholar

[27] S.G.Kumbar, R.James, S.P.Nukavarapu, C.T.Laurencin: Biomed. Mater.3 (3) (2008) 1. PMid: 18689924; 10.1088/1748-6041/3/3/034002Search in Google Scholar

[28] G.Freddi, P.Monti, M.Nagura, Y.Gotoh, M.Tsukada: Poly. Phys.35 (1997) 841. 10.1002/(SICI)1099-0488(19970415)35:5<841::AID-POLB13>3.0.CO;2-ASearch in Google Scholar

[29] H.Y.Kweon, I.C.Um, Y.H.Park: Polymer41 (2000) 7361. 10.1016/S0032-3861(00)00100-2Search in Google Scholar

[30] H.Y.Kweon, I.C.Um,Y.H.Park: Polymer42 (2001) 6651. 10.1016/S0032-3861(01)00104-5Search in Google Scholar

[31] W.U.Huawen, Th.Huser, A.Parikh, V.Yeh: Asia Optical Fiber Communication and Optoelectronic Exposition and Conference (AOE) (2008).Search in Google Scholar

[32] M.Z.Li, W.Tao, S.Kuga, Y.Nishiyama: Poly. Adv. Technol.1 (2003) 694. 10.1002/pat.409Search in Google Scholar

[33] T.Asakura, C.Tanaka, M.Yang, J.Yao, M.Kurokawa: Biomaterials25 (2004) 617. 10.1016/S0142-9612(03)00570-2Search in Google Scholar

Received: 2012-1-31
Accepted: 2012-8-27
Published Online: 2013-08-22
Published in Print: 2013-05-10

© 2013, Carl Hanser Verlag, München

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  6. The effect of nitrogen on the coarsening rate of precipitate phases in iron-based alloys with chromium and vanadium: experimental and theoretical investigations
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https://doi.org/10.3139/146.110888
Keywords for this article
Eri silk fibroin electro spun fibre; FTIR; TGA; SEM; Tissue engineering

Articles in the same Issue

  1. Contents
  2. Contents
  3. Original Contributions
  4. Martensite transformation of sub-micron retained austenite in ultra-fine grained manganese transformation-induced plasticity steel
  5. Isothermal transformation of β-phase in Cu-rich Cu-Al-Sn alloys
  6. The effect of nitrogen on the coarsening rate of precipitate phases in iron-based alloys with chromium and vanadium: experimental and theoretical investigations
  7. Phase diagram investigation of the Sn-InxAgyCuz (x:y:z = 7:2:1) section in the Ag-In-Sn-Cu system
  8. Grain refinement and mechanical properties of low-carbon steel by means of equal channel angular pressing and annealing
  9. Thermophysical properties of solid phase palladium over a wide temperature range
  10. Magnetic properties of Nd-Fe-Co-Al rapid solidification alloys
  11. Synthesis of Ir1-xRex (0.15 ≤ x ≤ 0.40) solid solutions under high-pressure and high-temperature
  12. Preparation and magnetic characterization of Fe/metal oxide nanocomposite particles by means of hydrogen reduction assisted ultrasonic spray pyrolysis (USP-HR)
  13. Biofilm formation and corrosion resistance of Ni/SiC nanocomposite layers
  14. Characterization of electrospun fibrous scaffold produced from Indian eri silk fibroin
  15. Hydrothermal synthesis of nano nickel phosphides and investigation of their thermal stability
  16. Effects of PVP and CTAB surfactants on the morphology of cerium oxide nanoparticles synthesized via co-precipitation method
  17. DGM News
  18. DGM News
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