Comparison of spectrophotometric and HPLC methods for determination of lipid peroxidation products in rat brain tissues
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M. Ďurfinová
,
M. Brechtlová
Abstract
Two methods for determination of lipid peroxidation (LPX) products in rat brain homogenates were compared. The thiobarbituric acid (TBA) test and HPLC assay for analysis of malondialdehyde (MDA) were applied. Rat brain homogenate dissolved in tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) was mixed with TBA and H3PO4 and heated at 100°C to form colored complex that was extracted into butanol. No significant differences were found between the contents of TBA-reacting substances and their amount deduced from the MDA-TBA analysis. The presented results show that LPX products in brain homogenates can be determined without interferences also by the TBA test. Moreover, a survey of various methods used for the sample preparation before analysis of LPX products originating from different brain areas was made and compared with the obtained results.
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© 2007 Institute of Chemistry, Slovak Academy of Sciences
Articles in the same Issue
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Articles in the same Issue
- Step by step towards understanding gold glyconanoparticles as elements of the nanoworld
- Structure—activity relationships for in vitro oxime reactivation of chlorpyrifos-inhibited acetylcholinesterase
- Separation and characterization of products from thermal cracking of individual and mixed polyalkenes
- Mobility of important toxic analytes in urban dust and simulated air filters determined by sequential extraction and GFAAS/ICP-OES methods
- Effect of addition of ameliorative materials on the distribution of As, Cd, Pb, and Zn in extractable soil fractions
- Effect of gamma irradiation on trichromatic values of spices
- Homo- and heteronuclear complexes of a new, vicinal dioxime ligand
- Synthesis of new triphenodithiazine- and indolocarbazolediones of biological interest
- Dielectric relaxation of butyl acrylate—alcohol mixtures using time domain reflectometry
- Ab initio study of small coinage metal telluride clusters AunTem (n, m = 1, 2)
- Binding affinities and spectra of complexes formed by dehydrotetrapyrido[20]annulene and small molecules
- Comparison of spectrophotometric and HPLC methods for determination of lipid peroxidation products in rat brain tissues
- Enantioselective extraction of mandelic acid enantiomers by L-dipentyl tartrate and β-cyclodextrin as binary chiral selectors
- Mechanism of thermal decomposition of cobalt acetate tetrahydrate
- Three-component, one-pot synthesis of 3,4-dihydropyrimidin-2-(1H)-ones catalyzed by bromodimethylsulfonium bromide
