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Study on ?-Galactosidase Isolation, Purification and Optimization of Lactose Hydrolysis in Whey for Production of Instant Energy Drink
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Alak Kumar Singh
Veröffentlicht/Copyright:
24. April 2009
Four yeast cultures, Kluyveromyces marxianus, Kluyveromyces fragilis, Saccharomyces marxianus and Saccharomyces fragilis, were selected for isolation of ?-galactosidase. Enzyme activity was measured for all four strains and found Kluyveromyces marxianus (177 ONPG unit/ml) to have the highest activity. Experimental whey was hydrolyzed with the enzyme extracted from K. marxianus, and the concentrations of enzyme used were 0.5, 1.0, 1.5, 2.0 and 2.5 ml of enzyme per litre of whey. The percentage of lactose hydrolysis was found to be a maximum of 80% in 60 minutes time for the enzyme concentration of 2.0 ml/ litre of whey.
Published Online: 2009-4-24
©2011 Walter de Gruyter GmbH & Co. KG, Berlin/Boston
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Artikel in diesem Heft
- Article
- The Effect of De-Oiled Rice Bran for Single Cell Protein Production Using Fungal Cultures under Solid State Fermentation
- Thermomechanical Property of Rice Kernels Studied by DMA
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- Study on ?-Galactosidase Isolation, Purification and Optimization of Lactose Hydrolysis in Whey for Production of Instant Energy Drink
- Drying Strategies for Fluidized-Bed Drying of Paddy
- Effect of Fines on Agglomeration in Spray Dryers: An Experimental Study
- Computer Modelling and Simulation of Bakeries' Production Planning
- Predictive Modeling of Salmonella Species Inactivation in Ground Pork and Turkey during Cooking
- The Effect of Moisture Content on Colour Characteristics of Walnuts
- Shorter Communication
- Effect of Pre-Treatment on the Drying Characteristics and Kinetics of Okra (Abelmoschus esculetus (L.) Moench) Slices
- Comparison of Angiotensin I-Converting Enzyme Inhibitor Activities of Pre-Fermented Douchi (a Chinese Traditional Fermented Soybean Food) Started with Various Cultures
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