In vitro anticancer and antibacterial potentials of selected medicinal plants and isolation and characterization of a natural compound from Withania coagulans

Bassam Oudh Aljohny; Yasir Anwar; Shahid Ali Khan

doi:10.1515/znc-2021-0259

Article

In vitro anticancer and antibacterial potentials of selected medicinal plants and isolation and characterization of a natural compound from Withania coagulans

Bassam Oudh Aljohny , Yasir Anwar and Shahid Ali Khan

Published/Copyright: January 14, 2022

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From the journal Zeitschrift für Naturforschung C Volume 77 Issue 7-8

Abstract

In the current study, five different plants, Syzygium Cumini, Fagonia cretica, Acacia modesta, Withania coagulans, and Olea europaea aqueous extracts were prepared and applied against the anticancer and antibacterial activities. It was observed that O. Europaea extract shows the highest anticancer activity with cell viability of 21.5%. All the five plants extract was also used against the inhibition of Bacillus subtilis where O. Europaea extract shows a promising inhibitory activity of 3.2 cm followed by W. coagulans. Furthermore, W. coagulans was subjected to the process of column chromatography as a result a withanolide was isolated. The fast atom bombardment mass spectrometry (FAB-MS) and high resolution fast atom bombardment (HRFAB-MS) [M + 1] indicated molecular weight at m/z 453 and molecular formula C₂₈H₃₇O₅. The UV–Vis. spectrum shows absorbance at 210 nm suggesting the presence of conjugated system, and Fourier-transform infrared spectroscopy (FTIR) was recorded to explore the functional groups. Similarly, 1D and 2D NMR spectroscopy techniques such as ¹H, ¹³C NMR, correlation spectroscopy (COSY-45°), heteronuclear single quantum correlation (HSQC), heteronuclear multiple bond correlation (HMBC) and Nuclear Overhauser effect Spectroscopy (NOESY) techniques was carried out to determine the unknown natural product. The collective data of all these techniques established the structure of the unknown compound and recognized as a withanolide.

Keywords: antibacterial activity; anticancer activity; O. Europaea ; Withania coagulans ; withanolide

Corresponding author: Shahid Ali Khan, Department of Chemistry, University of Swabi, Anbar 23561, Khyber Pakhtunkhwa, Pakistan, E-mail: shahidsawal007@gmail.com

Funding source: Deanship of Scientific Research (DSR), King Abdulaziz University, Jeddah

Award Identifier / Grant number: G-461-130-1440

Acknowledgments

This project was funded by the Deanship of Scientific Research (DSR) at King Abdulaziz University, under grant no. G:461-130-1440. The authors, therefore, acknowledge with thanks DSR for technical and financial support.

Author contributions: ‘BOA’ have made substantial contributions to the conception, the acquisition, analysis, and interpretation of data. ‘YA’ have made substantial contributions to the conception, the acquisition, analysis, and interpretation of data. ‘SAK’ have made substantial contributions to the conception; design of the work; the acquisition, analysis, interpretation of data; and have drafted the work. All authors have read and approved the manuscript.
Research funding: None declared.
Institutional review board statement: Not Applicable.
Informed consent: Not Applicable.
Data availability statement: All data and material are available upon request.
Conflict of interest statement: The authors declare no conflict of interest.

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/ZNC-2021-0259; www.mdpi.com/xxx/s1).

Received: 2021-10-13

Accepted: 2021-11-17

Published Online: 2022-01-14

Published in Print: 2022-07-26

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Supplementary Material

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Keywords for this article

antibacterial activity; anticancer activity; O. Europaea; Withania coagulans; withanolide