Home Life Sciences Chemical composition and anticholinesterase inhibitory activity of Pavetta graciliflora Wall. ex Ridl. essential oil
Article
Licensed
Unlicensed Requires Authentication

Chemical composition and anticholinesterase inhibitory activity of Pavetta graciliflora Wall. ex Ridl. essential oil

  • Wan Mohd Nuzul Hakimi Wan Salleh ORCID logo EMAIL logo and Shamsul Khamis
Published/Copyright: May 29, 2020

Abstract

Chemical composition and anticholinesterase activity of the essential oil of Pavetta graciliflora Wall. ex Ridl. (Rubiaceae) was examined for the first time. The essential oil was obtained by hydrodistillation and was fully characterized by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). A total of 20 components were identified in the essential oil, which made up 92.85% of the total oil. The essential oil is composed mainly of β-caryophyllene (42.52%), caryophyllene oxide (25.33%), β-pinene (8.67%), and α-pinene (6.52%). The essential oil showed weak inhibitory activity against acetylcholinesterase (AChE) (I%: 62.5%) and butyrylcholinesterase (BChE) (I%: 65.4%) assays. Our findings were shown to be very useful for the characterization, pharmaceutical, and therapeutic applications of the essential oil from P. graciliflora.

Acknowledgments

The authors would like to thank the Department of Chemistry, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris for use of their research facilities.

References

1. De Block P, Razafimandimbison SG, Janssens S, Ochoterena H, Robbrecht E, Bremer B. Molecular phylogenetics and generic assessment in the tribe Pavetteae (Rubiaceae). Taxon 2015;64:79–95.10.12705/641.19Search in Google Scholar

2. Lemaire B, Van Oevelen S, De Block P, Verstraete B, Smets E, Prinsen E, et al. Identification of the bacterial endosymbionts in leaf nodules of Pavetta (Rubiaceae). Int J Syst Evol Microbiol 2012;62:202–9.10.1099/ijs.0.028019-0Search in Google Scholar

3. Bariweni MW, Ozolua RI. Neuropharmacological effects of the aqueous leaf extract and fractions of Pavetta crassipes (K. Schum) Rubiaceae in mice. J Pharm Pharmacogn Res 2017;5:278–87.10.56499/jppres17.224_5.5.278Search in Google Scholar

4. Balde ES, Megalizzi V, Traore MS, Cos P, Maes L, Decaestecker C, et al. In vitro antiprotozoal, antimicrobial and antitumor activity of Pavetta crassipes K. Schum leaf extracts. J Ethnopharmacol 2010;130:529–35.10.1016/j.jep.2010.05.042Search in Google Scholar

5. Ramamoorthy J, Venkataraman S, Meera R, Chiristina AJ, Chidambaranathan N, Devi P, et al. Physio-phytochemical screening and diuretic activity of leaves of Pavetta indica Linn. J Pharm Sci Res 2010;2:506–12.Search in Google Scholar

6. Amos S, Okwuasaba FK, Gamaniel K, Akah P, Wambebe C. Inhibitory effects of the aqueous extract of Pavetta crassipes leaves on gastrointestinal and uterine smooth muscle preparations isolated from rabbits, guinea pigs and rats. J Ethnopharmacol 1998;61:209–13.10.1016/S0378-8741(98)00046-4Search in Google Scholar

7. Vahrmeijer J. Poisonous plants of southern Africa. Cape Town: Tafelberg, 1981.Search in Google Scholar

8. Balde AM, Pieters LA, Wray V, Kolodziej H, Berghe DAV, Claeys M, et al. Dimeric and trimeric proanthocyanidins possessing a doubly linked structure from Pavetta owariensis. Phytochemistry 1991;30:4129–35.10.1016/0031-9422(91)83480-9Search in Google Scholar

9. Nguyen YT-, Moon JY, Ryu JY, Eum S, Bach TT, Cho SK. Methanol extract of aerial parts of Pavetta indica L. enhances the cytotoxic effect of doxorubicin and induces radiation sensitization in MDA-MB-231 triple-negative breast cancer cells. Molecules 2019;24:2273–88.10.3390/molecules24122273Search in Google Scholar

10. Thayyil AH, Kottai-Muthu A. Comparative evaluation of in vitro antioxidant activities of various extracts from Chomelia asiatica (Linn) and Pavetta indica (Linn). J Pharm Sci Res 2018;10:2738–41.Search in Google Scholar

11. Ashwathanarayana R, Naika R. Anti-inflammatory properties of Pavetta crassicaulis Bremek. leaf and flower crude extracts and its pure compounds collected from Western Ghats, Karnataka, India. Asian J Pharm Clinical Res 2018;11:72–90.10.22159/ajpcr.2018.v11i9.21885Search in Google Scholar

12. Odumosu PO, Lough WJ, Yakubu D, Thomas K, Williamson G, Haroune N. Anti-mycobacterial assessment and characterization of 5-O-caffeoylquinic acid methyl ester and rutin from Pavetta crassipes. J Appl Pharm Sci 2016;6:1–7.10.7324/JAPS.2016.601001Search in Google Scholar

13. Natarajan P, Thangathirupathi A, Ramarajan S, Jaya S, Hareesh B, Laxminarayana G. Preliminary study of antidiabetic activity of methanolic extract of Pavetta indica Linn in diabetic rats. Asian J Pharm Clinical Res 2013;6:131–3.Search in Google Scholar

14. Rout RC, Deb DB. Taxonomic revision of the genus Pavetta (Rubiaceae) in Indian sub-continent. Bull Bot Surv 1999;41:1–182.Search in Google Scholar

15. Adams RP. Identification of essential oil components by gas chromatography-mass spectrometry. 4th ed. Carol Stream, IL: Allured Publishing Corporation, 2007.Search in Google Scholar

16. NIST08. Mass spectral library (NIST/EPA/NIH). Gaithersburg, USA: National Institute of Standards and Technology, 2008.Search in Google Scholar

17. FFNSC 2. Flavors and fragrances of natural and synthetic compounds. Mass spectral database. Japan: Shimadzu Corps., 2012.Search in Google Scholar

18. Ellman GL, Courtney KD, Andres V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 1961;7:88–95.10.1016/0006-2952(61)90145-9Search in Google Scholar

19. Salleh WM, Ahmad F, Yen KH. Chemical compositions and biological activities of the essential oils of Beilschmiedia madang Blume (Lauraceae). Arch Pharm Res 2015;38:485–93.10.1007/s12272-014-0460-zSearch in Google Scholar PubMed

20. Rao H, Lai P, Gao Y. Chemical composition, antibacterial activity, and synergistic effects with conventional antibiotics and nitric oxide production inhibitory activity of essential oil from geophila repens (L.) I.M. Johnst. Molecules 2017;22:1561–74.10.3390/molecules22091561Search in Google Scholar PubMed PubMed Central

21. François Prévost BK, Kablan L, Djeda R, Toure D, Konan F, Tia VE, et al. Chemical composition and evaluation of the antimicrobial activity of the essential oils from fruits of Morinda lucida. J Essent Oil Bear Pl 2018;21:905–12.10.1080/0972060X.2018.1517054Search in Google Scholar

22. Corey EJ, Mitra RB, Uda H. Total synthesis of d,l-caryophyllene and d,l-isocaryophyllene. J Am Chem Soc 1964;86:485–92.10.1021/ja01057a040Search in Google Scholar

23. Legault J, Pichette A. Potentiating effect of β-caryophyllene on anticancer activity of α-humulene, isocaryophyllene and paclitaxel. J Pharm Pharmacol 2007;59:1643–7.10.1211/jpp.59.12.0005Search in Google Scholar PubMed

24. Chavan MJ, Wakte PS, Shinde DB. Analgesic and anti-inflammatory activity of caryophyllene oxide from Annona squamosa L. bark. Phytomedicine 2010;17:149–51.10.1016/j.phymed.2009.05.016Search in Google Scholar PubMed

25. Sain S, Naoghare PK, Devi S, Daiwile A, Krishnamurthi K, Arrigo P, et al. Beta caryophyllene and caryophyllene oxide, isolated from Aegle marmelos, as the potent anti-inflammatory agents against lymphoma and neuroblastoma cells. Antiinflamm Antiallergy Agents Med Chem 2014;13:45–55.10.2174/18715230113129990016Search in Google Scholar PubMed

26. Kim C, Cho SK, Kapoor S, Kumar A, Vali S, Abbasi T, et al. β-Caryophyllene oxide inhibits constitutive and inducible STAT3 signaling pathway through induction of the SHP-1 protein tyrosine phosphatase. Mol Carcinog 2014;53:793–806.10.1002/mc.22035Search in Google Scholar PubMed

27. Raja Rajeswari N, RamaLakshmi S, Muthuchelian K. GC-MS analysis of bioactive components from the ethanolic leaf extract of Canthium dicoccum (Gaertn.) Teijsm & Binn. J Chem Pharm Res 2011;3:792–8.Search in Google Scholar

28. Dohi S, Terasaki M, Makino M. Acetylcholinesterase inhibitory activity and chemical composition of commercial essential oils. J Agric Food Chem 2009;57:4313–18.10.1021/jf804013jSearch in Google Scholar PubMed

29. Smail A, Lyoussi B, Maria G, Miguel. Antioxidant and antiacetylcholinesterase activities of some commercial essential oils and their major compounds. Molecules 2011;16:7672–90.10.3390/molecules16097672Search in Google Scholar PubMed PubMed Central

30. Masondo NA, Stafford GI, Aremu AO, Makunga NP. Acetylcholinesterase inhibitors from southern african plants: an overview of ethnobotanical, pharmacological potential and phytochemical research including and beyond Alzheimer’s disease treatment. South Afr J Bot 2019;120:39–64.10.1016/j.sajb.2018.09.011Search in Google Scholar

31. Orhan I, Sener B, Choudhary MI, Khalid A. Acetylcholinesterase and butyrylcholinesterase inhibitory activity of some Turkish medicinal plants. J Ethnopharmacol 2004;91:57–60.10.1016/j.jep.2003.11.016Search in Google Scholar

32. DeKosky ST, Harbaugh RE, Schmitt FA, Bakay RA-, Chui HC, Knopman DS, et al. Cortical biopsy in Alzheimer’s disease: diagnostic accuracy andneurochemical, neuropathological, and cognitive correlations. Ann Neurol 1992;32:625–32.10.1002/ana.410320505Search in Google Scholar

33. Baylac S, Racine R. Inhibition of 5-lipoxygenase by essential oils and other natural fragrant extracts. Int J Med Arom Pl 2003;13:138–42.10.1016/S0962-4562(03)00083-3Search in Google Scholar

Received: 2020-04-01
Revised: 2020-04-25
Accepted: 2020-05-12
Published Online: 2020-05-29
Published in Print: 2020-11-26

©2020 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Research articles
  3. Modulation of antibiotic resistance by the essential oil of Ocimum gratissimum L. in association with light-emitting diodes (LED) lights
  4. Dynamic analysis of the mathematical model of COVID-19 with demographic effects
  5. Biochemical evidences for M1-, M17- and M18-like aminopeptidases in marine invertebrates from Cuban coastline
  6. Hexanal inhalation affects cognition and anxiety-like behavior in mice
  7. Overexpression of a novel E3 ubiquitin ligase gene from Coptis chinensis Franch enhances drought tolerance in transgenic tobacco
  8. Topiroxostat ameliorates oxidative stress and inflammation in sepsis-induced lung injury
  9. A computational study of the interactions between anthocyans and cyclodextrins
  10. Chemical characterization of the lipids in femoral gland secretions of wild male tegu lizards, Salvator merianae (Squamata, Teiidae) in comparison with captive-bred males
  11. Metabolite profiling by means of GC-MS combined with principal component analyses of natural populations of Nectaroscordum siculum ssp. bulgaricum (Janka) Stearn
  12. human monoamine oxidase (hMAO) A and hMAO B inhibitors from Artemisia dracunculus L. herniarin and skimmin: human mononamine oxidase A and B inhibitors from A. dracunculus L.
  13. Rapid communications
  14. Chemical composition and anticholinesterase inhibitory activity of Pavetta graciliflora Wall. ex Ridl. essential oil
  15. Chemical composition of the essential oils of four Polyalthia species from Malaysia
  16. Composition of the essential oils of three Malaysian Xylopia species (Annonaceae)
  17. Chemical characterization of Goniothalamus macrophyllus and Goniothalamus malayanus leaves’ essential oils
  18. Effect of pH on xylitol production by Candida species from a prairie cordgrass hydrolysate
Downloaded on 6.1.2026 from https://www.degruyterbrill.com/document/doi/10.1515/znc-2020-0075/html?lang=en
Scroll to top button