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Biochemical evidences for M1-, M17- and M18-like aminopeptidases in marine invertebrates from Cuban coastline

  • Isel Pascual Alonso ORCID logo EMAIL logo , Laura Rivera Méndez ORCID logo , Mario E. Valdés-Tresanco , Lotfi Bounaadja , Marjorie Schmitt , Yarini Arrebola Sánchez , Luis Alvarez Lajonchere , Jean-Louis Charli ORCID logo and Isabelle Florent
Published/Copyright: June 30, 2020
Zeitschrift für Naturforschung C
From the journal Zeitschrift für Naturforschung C Volume 75 Issue 11-12

Abstract

Metallo-aminopeptidases (mAPs) control many physiological processes. They are classified in different families according to structural similarities. Neutral mAPs catalyze the cleavage of neutral amino acids from the N-terminus of proteins or peptide substrates; they need one or two metallic cofactors in their active site. Information about marine invertebrate’s neutral mAPs properties is scarce; available data are mainly derived from genomics and cDNA studies. The goal of this work was to characterize the biochemical properties of the neutral APs activities in eight Cuban marine invertebrate species from the Phyla Mollusca, Porifera, Echinodermata, and Cnidaria. Determination of substrate specificity, optimal pH and effects of inhibitors (1,10-phenanthroline, amastatin, and bestatin) and cobalt on activity led to the identification of distinct neutral AP-like activities, whose biochemical behaviors were similar to those of the M1 and M17 families of mAPs. Additionally, M18-like glutamyl AP activities were detected. Thus, marine invertebrates express biochemical activities likely belonging to various families of metallo-aminopeptidases.

Keywords: M1 family; M17 family; M18 family; marine invertebrate; metallo-aminopeptidase
Corresponding author: Isel Pascual Alonso, Center for Protein Studies, Faculty of Biology, University of Havana, 25 # 455, between J and I, Plaza de la Revolución, Havana, CP 10 400, Cuba, E-mail:

Funding source: International Union of Biochemistry and Molecular Biology

Funding source: Agence Nationale de la Recherche

Award Identifier / Grant number: ANR-12-BS07-0020-02 project MAMMAMIA: “design of potential anti MAlarial M1/M17 AMinopeptiIdase Agents”

Acknowledgments

The authors thank the IUBMB Mid-Career fellowship program (research of IPA at IF and JLC laboratories) and the French ANR-12-BS07-0020-02 project MAMMAMIA “design of potential antiMAlarial M1/M17 AMinopeptiIdase Agents”, 2012–2017. We also thank Prof. Jose Espinosa, from the National Institute of Oceanology, CITMA, Cuba, who kindly provided species pictures included in Figure 1 and in Supplementary Material, Figure 2.

  1. Author contribution: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: The authors thank the IUBMB Mid-Career fellowship program (research of IPA at IF and JLC laboratories) and the French ANR-12-BS07-0020-02 project MAMMAMIA “design of potential antiMAlarial M1/M17 AMinopeptiIdase Agents”, 2012–2017.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

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Supplementary material

The online version of this article offers supplementary material (https://doi.org/10.1515/ZNC-2019-0169).

Received: 2019-09-25
Accepted: 2020-06-01
Published Online: 2020-06-30
Published in Print: 2020-11-26

© 2020 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/znc-2019-0169
Keywords for this article
M1 family; M17 family; M18 family; marine invertebrate; metallo-aminopeptidase

Articles in the same Issue

  1. Frontmatter
  2. Research articles
  3. Modulation of antibiotic resistance by the essential oil of Ocimum gratissimum L. in association with light-emitting diodes (LED) lights
  4. Dynamic analysis of the mathematical model of COVID-19 with demographic effects
  5. Biochemical evidences for M1-, M17- and M18-like aminopeptidases in marine invertebrates from Cuban coastline
  6. Hexanal inhalation affects cognition and anxiety-like behavior in mice
  7. Overexpression of a novel E3 ubiquitin ligase gene from Coptis chinensis Franch enhances drought tolerance in transgenic tobacco
  8. Topiroxostat ameliorates oxidative stress and inflammation in sepsis-induced lung injury
  9. A computational study of the interactions between anthocyans and cyclodextrins
  10. Chemical characterization of the lipids in femoral gland secretions of wild male tegu lizards, Salvator merianae (Squamata, Teiidae) in comparison with captive-bred males
  11. Metabolite profiling by means of GC-MS combined with principal component analyses of natural populations of Nectaroscordum siculum ssp. bulgaricum (Janka) Stearn
  12. human monoamine oxidase (hMAO) A and hMAO B inhibitors from Artemisia dracunculus L. herniarin and skimmin: human mononamine oxidase A and B inhibitors from A. dracunculus L.
  13. Rapid communications
  14. Chemical composition and anticholinesterase inhibitory activity of Pavetta graciliflora Wall. ex Ridl. essential oil
  15. Chemical composition of the essential oils of four Polyalthia species from Malaysia
  16. Composition of the essential oils of three Malaysian Xylopia species (Annonaceae)
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