Topiroxostat ameliorates oxidative stress and inflammation in sepsis-induced lung injury

Haiying Fu; Junjie Zhang; Mayu Huang

doi:10.1515/znc-2020-0074

Article

Topiroxostat ameliorates oxidative stress and inflammation in sepsis-induced lung injury

Haiying Fu , Junjie Zhang and Mayu Huang

Published/Copyright: June 25, 2020

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From the journal Zeitschrift für Naturforschung C Volume 75 Issue 11-12

Abstract

Sepsis-induced lung injury was the most common cause of death in patients. Topiroxostat, a novel xanthine oxidoreductase inhibitors, possessed obvious organ protectives effects. Xanthine oxidase played a vital role in acute lung injury. The study aimed to investigate the roles of Topiroxostat in sepsis-induced lung injury. The sepsis rats were established using cecum ligation and perforation. The lung damage induced by sepsis was evaluated by Hematoxylin and Eosin staining and lung tissue wet to dry ratio. The oxidative stress was detected by measurement of reactive oxygen species, malondialdehyde, myeloperoxidase and superoxide dismutase (SOD). The pro-inflammatory mediators, tumor necrosis factor-α, interleukin (IL)-1β, IL-6 and monocyte chemotactic protein 1, were measured by Enzyme-Linked Immunosorbent Assay. The cell apoptosis in lung was detected by TUNNEL staining and western blot analysis of apoptosis-related proteins including pro-apoptosis proteins, Bax, cleaved caspase9, cleaved caspase3 and anti-apoptosis protein Bcl2. The results showed that Topiroxostat significantly reduced lung damage, along with decreased oxidative stress, inflammation response and apoptosis in sepsis rats. Topiroxostat exerted markedly protective effects in sepsis-induced lung injury and could be an antioxidant in treating sepsis-induced lung injury.

Keywords: apoptosis; inflammation; oxidative stress; sepsis; Topiroxostat

Corresponding author: Mayu Huang, Emergency Department, Tong Ren Hospital Shanghai Jiao Tong University School of Medicine, 1111 Xian xia Road Changning District, Shanghai, China. E-mail: MayuHuang12@163.com

The co-first authors: Haiying Fu, Junjie Zhang.

Acknowledgments

No.

Author contribution: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Declaration of Conflicting Interests: The authors declare that there is no conflict of interest.
Funding: The authors received no financial support for the research, authorship, and/or publication of this article.

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Received: 2020-03-31

Revised: 2020-04-28

Accepted: 2020-06-01

Published Online: 2020-06-25

Published in Print: 2020-11-26

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Articles in the same Issue

https://doi.org/10.1515/znc-2020-0074

Keywords for this article

apoptosis; inflammation; oxidative stress; sepsis; Topiroxostat