GDP-D-mannose pyrophosphorylase from Pogonatherum paniceum enhances salinity and drought tolerance of transgenic tobacco
-
Taobo Ai
and
Shenghua Wang
Abstract
Pogonatherum paniceum is a highly drought- and salt-tolerant plant species that is typically used for ecological restoration and the conservation of soil and water in many countries. Understanding the molecular mechanisms underlying plant abiotic stress responses, especially to salinity and drought stresses, in species such as P. paniceum could be important to broader crop improvement efforts. GDP-D-mannose pyrophosphorylase (GMPase) is the limiting enzyme in the synthesis of L-ascorbic acid (AsA), which plays a crucial role in the detoxification of reactive oxygen species (ROS). We have cloned and characterized the cDNA of the PpGMP gene of P. paniceum encoding a GMPase. The full-length cDNA sequence contains 1411 nucleotides encoding a putative protein with 361 amino acid residues and an approximate molecular mass of 39.68 kDa. The GMPase transcript was up-regulated in P. paniceum plants subjected to salinity and drought stress, respectively. Transgenic tobacco expressing PpGMPase exhibited enhanced salinity and drought resistance, a higher seed germination rate, better growth performance, a higher AsA content, a more stable redox state, higher superoxide dismutase (SOD) activity, and lower levels of malonaldehyde (MDA) and H2O2 under drought and salinity stress. Taken together, expression of PpGMPase in tobacco conferred salinity and drought stress tolerance by increasing the content of AsA, thereby enhancing ROS-detoxifying functions. Thus, PpGMP is a potential candidate gene for crop improvement.
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©2016 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- In vitro neuroprotective potential of the monoterpenes α-pinene and 1,8-cineole against H2O2-induced oxidative stress in PC12 cells
- Neem tree (Azadirachta indica) extract specifically suppresses the growth of tumors in H22-bearing Kunming mice
- Orofacial antinociceptive effect of the ethanolic extract of Annona vepretorum Mart. (Annonaceae)
- Identification and characterization of microRNAs and their target genes from Nile tilapia (Oreochromis niloticus)
- Chemotherapeutic effect of Berberis integerrima hydroalcoholic extract on colon cancer development in the 1,2-dimethyl hydrazine rat model
- New flavonoid C–O–C dimers and other chemical constituents from Garcinia brevipedicellata stem heartwood
- GDP-D-mannose pyrophosphorylase from Pogonatherum paniceum enhances salinity and drought tolerance of transgenic tobacco
- Hypoglycemic activity of Gleditsia caspica extract and its saponin-containing fraction in streptozotocin-induced diabetic rats
- Antinociceptive activity of Tibouchina pereirae, an endemic plant from the Brazilian semiarid region
- Heteroplasmy and atrazine resistance in Chenopodium album and Senecio vulgaris
- Extremely high boron tolerance in Puccinellia distans (Jacq.) Parl. related to root boron exclusion and a well-regulated antioxidant system
Articles in the same Issue
- Frontmatter
- In vitro neuroprotective potential of the monoterpenes α-pinene and 1,8-cineole against H2O2-induced oxidative stress in PC12 cells
- Neem tree (Azadirachta indica) extract specifically suppresses the growth of tumors in H22-bearing Kunming mice
- Orofacial antinociceptive effect of the ethanolic extract of Annona vepretorum Mart. (Annonaceae)
- Identification and characterization of microRNAs and their target genes from Nile tilapia (Oreochromis niloticus)
- Chemotherapeutic effect of Berberis integerrima hydroalcoholic extract on colon cancer development in the 1,2-dimethyl hydrazine rat model
- New flavonoid C–O–C dimers and other chemical constituents from Garcinia brevipedicellata stem heartwood
- GDP-D-mannose pyrophosphorylase from Pogonatherum paniceum enhances salinity and drought tolerance of transgenic tobacco
- Hypoglycemic activity of Gleditsia caspica extract and its saponin-containing fraction in streptozotocin-induced diabetic rats
- Antinociceptive activity of Tibouchina pereirae, an endemic plant from the Brazilian semiarid region
- Heteroplasmy and atrazine resistance in Chenopodium album and Senecio vulgaris
- Extremely high boron tolerance in Puccinellia distans (Jacq.) Parl. related to root boron exclusion and a well-regulated antioxidant system
