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Alkaloid profiles and acetylcholinesterase inhibitory activities of Fumaria species from Bulgaria

  • Radka Z. Vrancheva , Ivan G. Ivanov , Ina Y. Aneva , Ivayla N. Dincheva , Ilian K. Badjakov and Atanas I. Pavlov EMAIL logo
Published/Copyright: January 12, 2016
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Abstract

GC-MS analysis of alkaloid profiles of five Fumaria species, naturally grown in Bulgaria (F. officinalis, F. thuretii, F. kralikii, F. rostellata and F. schrammii) and analysis of acetylcholinesterase inhibitory activity of alkaloid extracts were performed. Fourteen isoquinoline alkaloids were identified, with the principle ones being protopine, cryptopine, sinactine, parfumine, fumariline, fumarophycine, and fumaritine. Protopine contents, defined by HPLC analysis varied between 210.6 ± 8.8 μg/g DW (F. schrammii) and 334.5 ± 7.1 μg/g DW. (F. rostellata). While all of the investigated alkaloid extracts significantly inhibited acetylcholinesterase activity, the F. kralikii demonstrated the highest level of inhibition (IC50 0.13 ± 0.01 mg extract/mL).


Corresponding author: Atanas I. Pavlov, Department of Analytical Chemistry, University of Food Technologies, Plovdiv, 26 Maritza Blvd., 4002, Plovdiv, Bulgaria; and Laboratory of Applied Biotechnologies, The Stephan Angeloff Institute of Microbiology, Department of Industrial Microbiology, Bulgarian Academy of Sciences, 139 Ruski Blvd., 4000 Plovdiv, Bulgaria, Phone/Fax: +359 32 642 430, E-mail:

Acknowledgments

The authors are grateful for the financial support of this research by the Bulgarian Science Foundation, Bulgarian Ministry of Education and Science under contract number: DMU 03/77- 2011.

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Received: 2014-10-13
Revised: 2015-8-5
Accepted: 2015-11-22
Published Online: 2016-1-12
Published in Print: 2016-1-1

©2016 by De Gruyter

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