Antiproliferative effects of triterpenoidal derivatives, obtained from the marine sponge Siphonochalina sp., on human hepatic and colorectal cancer cells
-
Ahmed Abdel-Lateff
,
Ahmed M. Al-Abd
Abstract
Three triterpenoidal derivatives [Sipholenol A (1), sipholenol L (2) and sipholenone A (3)] were isolated from the Red Sea sponge Siphonochalina sp. The structures were determined based on spectroscopic measurements (NMR, UV, IR and MS). The isolated compounds were evaluated for their cytotoxic activity against three cancer cell lines; HepG2, Caco-2 and HT-29. Moreover, the effects of these metabolites on cell cycle progression as well as cell cycle regulating proteins were assessed. Compounds 1, 2 and 3 showed moderate activity against HepG2 cells with IC50 values of 17.18 ± 1.18, 24.01 ± 0.59 and 35.06 ± 1.10 μM, respectively. Compounds 1 and 2 exerted a considerable antiproliferative effect with IC50 values of 4.80 ± 0.18 and 26.64 ± 0.30 μM, respectively, against Caco-2 cells. Finally, 1 and 2 exhibited antiproliferative activity against colorectal cancer cells (HT-29) with IC50 values of 24.65 ± 0.80 and 4.48 ± 0.1 μM, respectively. Cell cycle analysis indicated that these compounds induced cell cycle arrest particularly in G0/G1 and S phases. Furthermore, the triterpenoids increased the expression of cyclin-B1, cyclin-D1 and cleaved caspase-3, as determined by immunofluorescence, indicating an important role of apoptosis in cell death induced by these compounds.
Acknowledgments
The project was funded by the Deanship of Scientific Research (DSR), King Abdulaziz University, Jeddah under grant no. (353/166/1434). The authors, therefore, acknowledge with thanks DSR technical and financial support.
Conflict of interest statement: The authors declare that they have no actual or potential competing financial interests.
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Articles in the same Issue
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- Antiproliferative effects of triterpenoidal derivatives, obtained from the marine sponge Siphonochalina sp., on human hepatic and colorectal cancer cells
- Obituary
- Professor Peter Böger (23.03.1935 – 22.10.2015)
Articles in the same Issue
- Frontmatter
- Synergistic inhibitory effect of scopoletin and bisdemethoxycurcumin on Tetranychus cinnabarinus (Boisduval) (Acari: Tetranychidae)
- Alkaloid profiles and acetylcholinesterase inhibitory activities of Fumaria species from Bulgaria
- Unguisin F, a new cyclic peptide from the endophytic fungus Mucor irregularis
- The protective effect of daidzein on high glucose-induced oxidative stress in human umbilical vein endothelial cells
- Antiproliferative effects of triterpenoidal derivatives, obtained from the marine sponge Siphonochalina sp., on human hepatic and colorectal cancer cells
- Obituary
- Professor Peter Böger (23.03.1935 – 22.10.2015)
