The protective effect of daidzein on high glucose-induced oxidative stress in human umbilical vein endothelial cells

Mi Hwa Park; Jae-Won Ju; Mihyang Kim; Ji-Sook Han

doi:10.1515/znc-2015-0141

Article

The protective effect of daidzein on high glucose-induced oxidative stress in human umbilical vein endothelial cells

Mi Hwa Park , Jae-Won Ju , Mihyang Kim and Ji-Sook Han

Published/Copyright: January 12, 2016

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From the journal Zeitschrift für Naturforschung C Volume 71 Issue 1-2

Abstract

Endothelial cell dysfunction is considered a major cause of vascular complications in diabetes. In the present study, we investigated the protective effect of daidzein, a natural isoflavonoid, against high-glucose–induced oxidative damage in human umbilical vein endothelial cells (HUVECs). Treatment with a high concentration of glucose (30 mM) induced oxidative stress in the endothelial cells, against which daidzein protected the cells as demonstrated by significantly increased cell viability. In addition, lipid peroxidation, intracellular reactive oxygen species (ROS) generation, and indirect nitric oxide levels induced by the high glucose treatment were significantly reduced in the presence of daidzein (0.02–0.1 mM) in a dose-dependent manner. High glucose levels induced the overexpression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and NF-κB proteins in HUVECs, which was suppressed by treatment with 0.04 mM daidzein. These findings indicate the potential of daidzein to reduce high glucose-induced oxidative stress.

Keywords: daidzein; high glucose; HUVECs; oxidative stress; protective effects

Corresponding author: Ji-Sook Han, Department of Food Science and Nutrition and Research Institute of Ecology for the Elderly, Pusan National University, Busan 609-735, Republic of Korea, Phone: +82 51 510 2836, Fax: +82 51 583 3648; E-mail: hanjs@pusan.ac.kr

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Received: 2015-6-5

Revised: 2015-12-3

Accepted: 2015-12-3

Published Online: 2016-1-12

Published in Print: 2016-1-1

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https://doi.org/10.1515/znc-2015-0141

Keywords for this article

daidzein; high glucose; HUVECs; oxidative stress; protective effects