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Extraction, isolation and characterization of secondary metabolites in the leaves of Morinda lucida from Oshiegbe in Ebonyi State

  • Dorathy Chinasa Nwokonkwo ORCID logo and Emmanuel Ikechukwu Nwafor ORCID logo EMAIL logo
Published/Copyright: April 28, 2022
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Physical Sciences Reviews
From the journal Physical Sciences Reviews Volume 8 Issue 11

Abstract

This research work assessed the extraction, isolation and characterization of secondary metabolites in the leaves of Morinda lucida. The leaves of the plant were collected from Oshiegbe in Ebonyi state. They were air-dried and pulverized for extraction. The extraction was done using petroleum ether, ethyl acetate and ethanol through sequential extraction method at room temperature. The extracts were concentrated and subjected to phytochemical analysis. The concentrated fraction was subjected to thin layer chromatography (TLC) and column chromatography to obtain the pure compounds. The isolated compounds were characterized using ultraviolet/visible spectroscopy (UV), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance spectroscopy (NMR) and gas chromatography-mass spectroscopy (GC-MS) for their structural elucidation. The result of the work showed that the ethanol extract recorded the highest percentage yield of 5.36 while the petroleum ether extract recorded the least percentage yield of 0.61. The preliminary phytochemical screening of the extracts revealed the presence of alkaloids, saponins, tannins, terpenoids, flavonoids, steroids and glycosides in the leaves of the plants. The ethanol extract yielded four pure isolates (compounds) of R f values: 0.93, 0.80, 0.86 and 0.72. The compounds were characterized as N-ethyl-N-nitroso-ethylmethanoate (1) bicyclo-[3,1,1]-2,6,6-trimethyl heptane-2,3-diol (2) dibutylphthalate (3) and 1,2-benzenedicarboxylic acid-butyl-2-methylpropyl ester (4) The spectral results and GC-MS fragmentation pattern agreed with the structures of the isolated compounds.

Keywords: characterization; extraction; isolated compounds; Morinda lucida; phytochemicals; screening and fragmentation; secondary metabolites
Corresponding author: Emmanuel Ikechukwu Nwafor, Department of Industrial Chemistry, Ebonyi State University, Abakaliki, Nigeria, E-mail:

Funding source: Iykolysis Scientific Ventures Nig. Ltd

Acknowledgements

We express our warm gratitude to Dr. Adeyi E.O. and Mr. Ajayi J.O. of Chemistry Department, University of Ibadan, Nigeria for allowing us to use the Departmental laboratory for the research analysis.

  1. Author contribution: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: This work was funded by Iykolysis Scientific Ventures Nig. Ltd.

  3. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

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Published Online: 2022-04-28

© 2022 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/psr-2021-0226
Keywords for this article
characterization; extraction; isolated compounds; Morinda lucida; phytochemicals; screening and fragmentation; secondary metabolites

Articles in the same Issue

  1. Frontmatter
  2. Reviews
  3. Anticancer properties of arylchromenes and arylchromans: an overview
  4. Solid state lithium ion conductors for lithium batteries
  5. Performance and kinetics of a fluidized bed anaerobic reactor treating distillery effluent
  6. Use of biochemical markers for diabetes prevention in the new decade
  7. Antibreast cancer activities of phytochemicals from Anonna muricata using computer-aided drug design (CADD) approach
  8. Alkaline-earth metal(II) complexes of salinomycin – spectral properties and antibacterial activity
  9. Use of heterogeneous catalysis in sustainable biofuel production
  10. Antibacterial, antioxidant and cytotoxic activities of the stem bark of Archidendron jiringa (Jack) I.C. Nielsen
  11. A review of sludge production in South Africa municipal wastewater treatment plants, analysis of handling cost and potential minimization methods
  12. Cu-Catalysed tandem reactions for building poly hetero atom heterocycles-green chemistry tool
  13. Optimizing Cr(VI) adsorption parameters on magnetite (Fe3O4) and manganese doped magnetite (MnxFe(3-x)O4) nanoparticles
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  15. A novel application of synthesised based squarylium dyes on nylon 6, and silk woven fabrics
  16. Chromatographic characterization of the fusion protein SARS-CoV-2 S protein (RBD)-hFc
  17. Ethnopharmacology, phytochemistry and a new chemotaxonomic marker in Oldenlandia affinis (Roem. & Schult.) DC. Rubiaceae
  18. Extraction, isolation and characterization of secondary metabolites in the leaves of Morinda lucida from Oshiegbe in Ebonyi State
  19. Lead optimisation efforts on a molecular prototype of the immunomodulatory parasitic protein ES-62
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  21. Synthesis, characterization, DFT and molecular docking studies of acetone O-((2,5-dichlorophenyl)sulfonyl) oxime
  22. Design of membrane systems
  23. Conceptual design and cost-efficient environmentally Benign synthesis of beta-lactams
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  25. The spontaneity of chemical reactions: challenges with handling the concept and its implications
  26. Copper nanoparticles catalyzed carbon–heteroatom bond formation and synthesis of related heterocycles by greener procedures
  27. Tellurium in carbohydrate synthesis
  28. Conformational preferences and intramolecular hydrogen bonding patterns of tetraflavaspidic acid BBBB – a tetrameric acylphloroglucinol
  29. Phytochemical and antioxidant studies of Hibiscus Cannabinus seed oil
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