Startseite Changes in circMyt1l/rno-let-7d-5p/brain-derived neurotrophic factor. A damaged periventricular white matter damage model in neonatal rats
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Changes in circMyt1l/rno-let-7d-5p/brain-derived neurotrophic factor. A damaged periventricular white matter damage model in neonatal rats

  • Lihua Zhu EMAIL logo , Yiwen Han und Jiaping Shu
Veröffentlicht/Copyright: 9. November 2023
Journal of Perinatal Medicine
Aus der Zeitschrift Journal of Perinatal Medicine Band 52 Heft 1

Abstract

Objectives

To investigate the function of circMyt1l/rno-let-7d-5p/BDNF in the white matter damage of premature rats.

Methods

Bioinformatic analysis was used to analyze the differential expression of circMyt1l and its interacting miRNAs and mRNAs in rats with periventricular white matter damage. Rats at postnatal day 3 had their right common carotid artery permanently ligated, and were then exposed for 2 h to 6 % O2, or sham surgery and exposure to normal O2 levels (sham). CircMyt1l and rno-let-7d-5p expression was detected and BDNF protein levels were analyzed at 24, 48, and 72 h post hypoxia–ischemia.

Results

Bioinformatic analysis suggested that circMyt1l, rno-let-7d-5p and BDNF interact. CircMyt1l expression decreased significantly relative to the sham-operated rats (p<0.01) in an exposure time-dependent manner. Contrastingly, rno-let-7d-5p increased significantly relative to the sham-operated rats (p<0.01) in an exposure time dependent manner. BDNF protein levels decreased significantly relative to the sham-operated rats (p<0.05) in an exposure time dependent manner.

Conclusions

The expression levels of circMyt1l/rno-let-7d-5p/BDNF are interrelated in periventricular white matter damage. Decreased circMyt1l expression of promoted the effect of rno-let-7d-5p and decreased the level of its target, BDNF.

Keywords: circRNA; BDNF; white matter damage; premature; hypoxia–ischemia
Corresponding author: Lihua Zhu, Jiangsu Health Vocational College, 69 Huangshan Ling Road, Pukou District, Nanjing 211800, Jiangsu, P.R. China, Phone: +86 25 68172741, E-mail:
Lihua Zhu and Yiwen Han contributed equally to this work.

Funding source: Medical Research Project of Jiangsu Provincial Health Committee

Award Identifier / Grant number: H2019003

Funding source: Qinglan Project of Jiangsu Province of China

Award Identifier / Grant number: Jiangsu Teacher Letter (2021) 11

  1. Research ethics: This research has complied with all relevant national regulations and institutional policies and has been approved by Jiangsu Health Vocational College Animal Experimentation Committee.

  2. Informed consent: Not applicable.

  3. Author contributions: Each author meet all four criteria of authorship: 1. Substantial contributions to the conception or design of the work; the acquisition, analysis, or interpretation of data for the work; and 2. Drafting the work or revising it critically for important intellectual content; and 3. Final approval of the version to be published; and 4. Agreement to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

  4. Competing interests: The authors state no conflict of interest.

  5. Research funding: This work was supported by the Qinglan Team of Universities in Jiangsu Province [grant number: Jiangsu Teacher Letter (2021) 11]. Medical Research Project of Jiangsu Provincial Health Committee [grant number: H2019003].

  6. Data availability: Not applicable.

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Received: 2023-07-31
Accepted: 2023-10-20
Published Online: 2023-11-09
Published in Print: 2024-01-29

© 2023 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Review
  3. Human papillomavirus prevalence in pregnant women living with human immunodeficiency virus infection: a scoping review of the literature
  4. Original Articles – Obstetrics
  5. Enhanced recovery after cesarean from the patient perspective: a prospective study of the ERAC Questionnaire (ERAC-Q)
  6. Outcomes of cesarean delivery in placenta accreta: conservative delivery vs. cesarean hysterectomy
  7. Maternal and neonatal complications in pregnancies with and without pre-gestational diabetes mellitus
  8. Down-regulated Wnt7a and GPR124 in early-onset preeclampsia placentas reduce invasion and migration of trophoblast cells
  9. Pregnancy, delivery and neonatal outcomes in women with gastrointestinal system cancer in pregnancy. An evaluation of a population database
  10. Paternal age and perinatal outcomes: an observational study
  11. The association between maternal colonization with Group B Streptococcus and infectious morbidity following transcervical Foley catheter-assisted labor induction
  12. Hepatic arterial buffer response: activation in donor fetuses and the effect of laser ablation of intertwin anastomosis
  13. Comparison between trans-vaginal and trans-abdominal ultrasound examination of the cervix in the second trimester of pregnancy: a prospective study
  14. Collaboration between maternal-fetal medicine and family planning: a survey of Northeast US academic medical centers
  15. Original Articles – Fetus
  16. Association of low pregnancy associated plasma protein-A with increased umbilical artery pulsatility index in cases of fetal weight between the 3rd and 10th percentiles: a retrospective cohort study
  17. Genetic analysis, ultrasound phenotype, and pregnancy outcomes of fetuses with Xp22.33 or Yp11.32 microdeletions
  18. Original Articles – Neonates
  19. Umbilical cord blood hematological parameters in predicting early onset neonatal sepsis (EOS) – a prospective cohort study
  20. Changes in circMyt1l/rno-let-7d-5p/brain-derived neurotrophic factor. A damaged periventricular white matter damage model in neonatal rats
  21. Short Communication
  22. Effects of gender on fetal cortical development: a secondary analysis of a prospective cross-sectional study
  23. Acknowledgment
  24. Acknowledgment
https://doi.org/10.1515/jpm-2023-0311
Schlagwörter für diesen Artikel
circRNA; BDNF; white matter damage; premature; hypoxia–ischemia

Artikel in diesem Heft

  1. Frontmatter
  2. Review
  3. Human papillomavirus prevalence in pregnant women living with human immunodeficiency virus infection: a scoping review of the literature
  4. Original Articles – Obstetrics
  5. Enhanced recovery after cesarean from the patient perspective: a prospective study of the ERAC Questionnaire (ERAC-Q)
  6. Outcomes of cesarean delivery in placenta accreta: conservative delivery vs. cesarean hysterectomy
  7. Maternal and neonatal complications in pregnancies with and without pre-gestational diabetes mellitus
  8. Down-regulated Wnt7a and GPR124 in early-onset preeclampsia placentas reduce invasion and migration of trophoblast cells
  9. Pregnancy, delivery and neonatal outcomes in women with gastrointestinal system cancer in pregnancy. An evaluation of a population database
  10. Paternal age and perinatal outcomes: an observational study
  11. The association between maternal colonization with Group B Streptococcus and infectious morbidity following transcervical Foley catheter-assisted labor induction
  12. Hepatic arterial buffer response: activation in donor fetuses and the effect of laser ablation of intertwin anastomosis
  13. Comparison between trans-vaginal and trans-abdominal ultrasound examination of the cervix in the second trimester of pregnancy: a prospective study
  14. Collaboration between maternal-fetal medicine and family planning: a survey of Northeast US academic medical centers
  15. Original Articles – Fetus
  16. Association of low pregnancy associated plasma protein-A with increased umbilical artery pulsatility index in cases of fetal weight between the 3rd and 10th percentiles: a retrospective cohort study
  17. Genetic analysis, ultrasound phenotype, and pregnancy outcomes of fetuses with Xp22.33 or Yp11.32 microdeletions
  18. Original Articles – Neonates
  19. Umbilical cord blood hematological parameters in predicting early onset neonatal sepsis (EOS) – a prospective cohort study
  20. Changes in circMyt1l/rno-let-7d-5p/brain-derived neurotrophic factor. A damaged periventricular white matter damage model in neonatal rats
  21. Short Communication
  22. Effects of gender on fetal cortical development: a secondary analysis of a prospective cross-sectional study
  23. Acknowledgment
  24. Acknowledgment
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