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The effect of maternal diabetes on the expression of gamma-aminobutyric acid and metabotropic glutamate receptors in male newborn rats’ inferior colliculi

  • Maryam Ghenaatgar-Kasbi ORCID logo , Mohammad Rezaei ORCID logo , Payam Moharreri ORCID logo , Hamid Heidarian Miri ORCID logo , Ghasem Sazegar ORCID logo and Hossein Haghir ORCID logo EMAIL logo
Published/Copyright: May 5, 2023
Journal of Perinatal Medicine
From the journal Journal of Perinatal Medicine Volume 51 Issue 7

Abstract

Objectives

Few studies have examined the molecular alterations in the auditory pathway of infants of diabetic mothers, notwithstanding the fact that maternal diabetes may have an impact on the development of the neonatal peripheral and central nervous systems. Male newborn rats were studied to determine how maternal diabetes affected the expression of gamma-aminobutyric acid (GABAAα1 and GABAB1) and metabotropic glutamate (mGlu2) receptors in the inferior colliculus (IC) in this research.

Methods

Female rats were given a single intraperitoneal injection of streptozotocin (STZ) at a 65 mg/kg dose to develop a model of diabetic mothers. The study population was split into sham, diabetes without treatment, and diabetes with insulin groups. Their male neonatal rats were anesthetized on P0, P7, and P14 after mating and delivery. The receptors’ distribution pattern was studied using immunohistochemistry (IHC).

Results

Pairwise comparison in the groups revealed that the GABA receptors (Aα1 and B1) were significantly downregulated in the diabetes without treatment group (p<0.001). Furthermore, pairwise comparison in the groups indicated significant mGlu2 upregulation in the diabetes without treatment group (p<0.001). Regarding the concentration of all receptors, there was no discernible distinction between the diabetes with insulin and sham groups.

Conclusions

This investigation showed that the concentration of GABAAα1 and GABAB1 receptors decreased significantly over time, whereas the concentration of mGlu2 receptors increased significantly over time in male neonatal rats born to streptozotocin-induced diabetic mothers.

Keywords: diabetes; GABA; inferior colliculus; mGlu; neonatal; rat
Corresponding author: Hossein Haghir, Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; and Medical Genetic Research Center (MGRC), School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran, E-mail:

Funding source: Mashhad University of Medical Sciences

Award Identifier / Grant number: 981584

Acknowledgments

Authors are grateful to Dr. Somaye Fallahnezhad, Dr. Hamideh Babaloo, and Dr. Fatemeh Tahmasebi.

  1. Research funding: The study is the results of Maryam Ghenaatgar-Kasbi’s MSc thesis. Deputy Research of Mashhad University of Medical Sciences (MUMS) funded this study with a grant number of 981584.

  2. Author contribution: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Not applicable.

  5. Ethical approval: The Ethics Committee of the Mashhad University of Medical Sciences approved this research (IR.MUMS.MEDICAL.REC.1399.446).

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Received: 2022-11-14
Accepted: 2023-02-28
Published Online: 2023-05-05
Published in Print: 2023-09-26

© 2023 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/jpm-2022-0553
Keywords for this article
diabetes; GABA; inferior colliculus; mGlu; neonatal; rat

Articles in the same Issue

  1. Frontmatter
  2. Reviews
  3. Covid-19 vaccination and pregnancy: a systematic review of maternal and neonatal outcomes
  4. Improvised bubble continuous positive airway pressure ventilation use in neonates in resource-limited settings: a systematic review and meta-analysis
  5. Opinion Papers
  6. Anger: an underappreciated destructive force in healthcare
  7. Severe maternal thrombocytopenia and prenatal invasive procedures: still a grey zone
  8. Commentary
  9. The care of the magic of life before and after its beginning
  10. Original Articles – Obstetrics
  11. The impact of trimester of COVID-19 infection on pregnancy outcomes after recovery
  12. Adverse outcomes and maternal complications in pregnant women with severe-critical COVID-19: a tertiary center experience
  13. Are bacteria, fungi, and archaea present in the midtrimester amniotic fluid?
  14. Bioavailability of the tumor necrosis factor alpha/regulated on activation, normal T cell expressed and secreted (RANTES) biosystem inside the gestational sac during the pre-immune stages of embryo development
  15. The role of the soluble fms-like tyrosine kinase-1/placental growth factor (sFlt-1/PIGF) – ratio in clinical practice in obstetrics: diagnostic and prognostic value
  16. Prenatal diagnosis of non-mosaic sex chromosome abnormalities: a 10-year experience from a tertiary referral center
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