The neuroprotective effect of magnesium sulfate in preterm fetal mice
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Geum Joon Cho
und
Hai-Joong Kim
Abstract
Objective: To investigate the use of magnesium sulfate (MgSO4) as a neuroprotective agent in a mouse model of inflammation-associated and noninflammation-associated preterm birth.
Methods: On embryonic day 15 of gestation, lipopolysaccharide (LPS) and mifepristone (RU486) were used, respectively, to create mouse models of inflammation and noninflammation-associated preterm birth. After intraperitoneal injection of LPS, RU486, or normal saline solution (NS), dams were randomized to intraperitoneal MgSO4 or NS injection. From the 6 treatment groups (NS+NS, LPS+NS, NS+MgSO4, LPS+MgSO4, RU486+NS and RU486+MgSO4), fetal brains were collected for Western blot analysis and neuronal cultures. Protein expression of S100B was assessed, and immunohistochemistry was performed to detect NeuN. The numbers of NeuN-labeled cells were counted using confocal laser scanning microscopy.
Results: The expression of S100B significantly differed among the groups and was decreased in the LPS+MgSO4 group compared to the LPS+NS group. The expression of S100B did not differ between the RU486+NS and RU486+MgSO4 groups. NeuN-labeled cells were increased in the LPS+MgSO4 group compared with the LPS+NS group. NeuN-labeled cells were decreased in the RU486+MgSO4 group compared with the RU486+NS group.
Conclusions: We observed that prenatal treatment with MgSO4 was associated with decreased expression of S100B and increased numbers of NeuN-labeled cells in an inflammation-associated preterm mouse model but not in a noninflammation-associated preterm mouse model. Our results suggest that prenatal treatment of MgSO4 reduces inflammation-associated brain injury in fetal mice.
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The authors stated that there are no conflicts of interest regarding the publication of this article.
©2015 by De Gruyter
Artikel in diesem Heft
- Frontmatter
- Editorial
- Risk factors for preterm birth and new approaches to its early diagnosis
- Review
- Avoiding the prenatal programming effects of glucocorticoids: are there alternative treatments for the induction of antenatal lung maturation?
- Original articles – Obstetrics
- The association of first trimester bleeding with preterm delivery
- Cervical sonoelastography for improving prediction of preterm birth compared with cervical length measurement and fetal fibronectin test
- The neuroprotective effect of magnesium sulfate in preterm fetal mice
- Correlation of cervical length, fetal fibronectin, phIGFBP-1, and cytokines in spontaneous preterm birth up to 14 days from sampling
- The unborn smoker: association between smoking during pregnancy and adverse perinatal outcomes
- Association of group B streptococcus colonization with early term births
- Association of maternal whole blood fatty acid status during the prenatal period with term birth dimensions: a cross-sectional study
- Predicting onset of labor from echogenicity of the cervical gland area on vaginal ultrasonography at term
- Disparity in post-treatment maternal circulating magnesium sulfate levels between twin and singleton gestation: Is this the missing link between plurality and adverse outcome?
- Original articles – Fetus
- Cardiovascular profile score in 44 fetuses with cervicofacial tumors
- First trimester tricuspid regurgitation and fetal abnormalities
- Gastroschisis: incidence and prediction of growth restriction
- Original articles – Newborn
- Diminished HLA-DR expression on monocyte and dendritic cell subsets indicating impairment of cellular immunity in pre-term neonates: a prospective observational analysis
- First- and fifth-minute Apgar scores of 0–3 and infant mortality: a population-based study in São Paulo State of Brazil
- Feeding the feeble: steps towards nourishing preterm infants
- Book review
- Hot topics in perinatal medicine
- Erratum
- Erratum to: Impact of the nitric oxide-donor pentaerythrityl-tetranitrate on perinatal outcome in risk pregnancies: a prospective, randomized, double-blinded trial
- Congress Calendar
- Congress Calendar
Artikel in diesem Heft
- Frontmatter
- Editorial
- Risk factors for preterm birth and new approaches to its early diagnosis
- Review
- Avoiding the prenatal programming effects of glucocorticoids: are there alternative treatments for the induction of antenatal lung maturation?
- Original articles – Obstetrics
- The association of first trimester bleeding with preterm delivery
- Cervical sonoelastography for improving prediction of preterm birth compared with cervical length measurement and fetal fibronectin test
- The neuroprotective effect of magnesium sulfate in preterm fetal mice
- Correlation of cervical length, fetal fibronectin, phIGFBP-1, and cytokines in spontaneous preterm birth up to 14 days from sampling
- The unborn smoker: association between smoking during pregnancy and adverse perinatal outcomes
- Association of group B streptococcus colonization with early term births
- Association of maternal whole blood fatty acid status during the prenatal period with term birth dimensions: a cross-sectional study
- Predicting onset of labor from echogenicity of the cervical gland area on vaginal ultrasonography at term
- Disparity in post-treatment maternal circulating magnesium sulfate levels between twin and singleton gestation: Is this the missing link between plurality and adverse outcome?
- Original articles – Fetus
- Cardiovascular profile score in 44 fetuses with cervicofacial tumors
- First trimester tricuspid regurgitation and fetal abnormalities
- Gastroschisis: incidence and prediction of growth restriction
- Original articles – Newborn
- Diminished HLA-DR expression on monocyte and dendritic cell subsets indicating impairment of cellular immunity in pre-term neonates: a prospective observational analysis
- First- and fifth-minute Apgar scores of 0–3 and infant mortality: a population-based study in São Paulo State of Brazil
- Feeding the feeble: steps towards nourishing preterm infants
- Book review
- Hot topics in perinatal medicine
- Erratum
- Erratum to: Impact of the nitric oxide-donor pentaerythrityl-tetranitrate on perinatal outcome in risk pregnancies: a prospective, randomized, double-blinded trial
- Congress Calendar
- Congress Calendar
